Results 1 to 10 of about 38,220 (259)

In vitro and in vivo exploration of the cellobiose and cellodextrin phosphorylases panel in Ruminiclostridium cellulolyticum: implication for cellulose catabolism [PDF]

Biotechnology for Biofuels, 2019
Background In anaerobic cellulolytic micro-organisms, cellulolysis results in the action of several cellulases gathered in extracellular multi-enzyme complexes called cellulosomes.
Nian Liu, Aurélie Fosses, Clara Kampik, Goetz Parsiegla, Yann Denis, Nicolas Vita, Henri-Pierre Fierobe, Stéphanie Perret   +7 more
doaj   +5 more sources

Insights to improve the activity of glycosyl phosphorylases from Ruminococcus albus 8 with cello-oligosaccharides [PDF]

Frontiers in Chemistry, 2023
The phosphorolysis of cello-oligosaccharides is a critical process played in the rumen by Ruminococcus albus to degrade cellulose. Cellodextrins, made up of a few glucosyl units, have gained lots of interest by their potential applications.
Alem Storani, Sergio A. Guerrero, Alberto A. Iglesias   +2 more
doaj   +2 more sources

Efficient Biocatalytic Synthesis of Dihalogenated Purine Nucleoside Analogues Applying Thermodynamic Calculations [PDF]

Molecules, 2020
The enzymatic synthesis of nucleoside analogues has been shown to be a sustainable and efficient alternative to chemical synthesis routes. In this study, dihalogenated nucleoside analogues were produced by thermostable nucleoside phosphorylases in ...
Heba Yehia, Sarah Westarp, Viola Röhrs, Felix Kaspar, Robert T. Giessmann, Hendrik F.T. Klare, Katharina Paulick, Peter Neubauer, Jens Kurreck, Anke Wagner   +9 more
doaj   +3 more sources

Nucleoside Phosphorylases make N7-xanthosine [PDF]

Nature Communications
Modern, highly evolved nucleoside-processing enzymes are known to exhibit perfect regioselectivity over the glycosylation of purine nucleobases at N9. We herein report an exception to this paradigm.
Sarah Westarp, Felix Brandt, Lena Neumair, Christina Betz, Amin Dagane, Sebastian Kemper, Christoph R. Jacob, Peter Neubauer, Anke Kurreck, Felix Kaspar   +9 more
doaj   +2 more sources

Engineering a Bifunctional Fusion Purine/Pyrimidine Nucleoside Phosphorylase for the Production of Nucleoside Analogs [PDF]

Biomolecules
Nucleoside phosphorylases (NPs) are pivotal enzymes in the salvage pathway, catalyzing the reversible phosphorolysis of nucleosides to produce nucleobases and α-D-ribose 1-phosphate.
Daniel Hormigo, Jon Del Arco, Javier Acosta, Maximilian J. L. J. Fürst, Jesús Fernández-Lucas   +4 more
doaj   +2 more sources

Bacterial Purine Nucleoside Phosphorylases from Mesophilic and Thermophilic Sources: Characterization of Their Interaction with Natural Nucleosides and Modified Arabinofuranoside Analogues [PDF]

Biomolecules
The enzymatic synthesis of nucleoside derivatives is an important alternative to multi-step chemical methods traditionally used for this purpose. Despite several undeniable advantages of the enzymatic approach, there are a number of factors limiting its ...
Irina A. Bychek, Anastasia A. Zenchenko, Maria A. Kostromina, Marat M. Khisamov, Pavel N. Solyev, Roman S. Esipov, Sergey N. Mikhailov, Irina V. Varizhuk   +7 more
doaj   +2 more sources

Biosynthesis of Arabinoside from Sucrose and Nucleobase via a Novel Multi-Enzymatic Cascade [PDF]

Biomolecules
Arabinoside and derived nucleoside analogs, a family of nucleoside analogs, exhibit diverse typically biological activities and are widely used as antibacterial, antiviral, anti-inflammatory, antitumor, and other drugs in clinical and preclinical trials.
Yuxue Liu, Erchu Yang, Xiaojing Zhang, Xiaobei Liu, Xiaoting Tang, Zhenyu Wang, Hailei Wang   +6 more
doaj   +2 more sources

One-pot synthesis of cellobiose from sucrose using sucrose phosphorylase and cellobiose phosphorylase co-displaying Pichia pastoris as a reusable whole-cell biocatalyst [PDF]

Scientific Reports
Cellobiose has received increasing attention in various industrial sectors, ranging from food and feed to cosmetics. The development of large-scale cellobiose applications requires a cost-effective production technology as currently used methods based on
Kentaro Inokuma, Kiyotsuna Toyohara, Tomoya Hamada, Akihiko Kondo, Tomohisa Hasunuma   +4 more
doaj   +2 more sources

Synthesis of 5-oxymethyl-1,2,4-triazole-3-carboxamides

Тонкие химические технологии, 2022
Objectives. A key step in the synthesis of natural nucleoside analogs is the formation of a glycosidic bond between the carbohydrate fragment and the heterocyclic base. Glycosylation methods differ in terms of regio- and stereoselectivity.
L. E. Grebenkina, A. N. Prutkov, A. V. Matveev, M. V. Chudinov   +3 more
doaj   +1 more source

SOME RECENT FINDINGS IN THE BIOTECHNOLOGY OF BIOLOGICALLY IMPORTANT NUCLEOSIDES [PDF]

Biotechnologia Acta, 2013
Some recent findings in the biotechnology of biologically important nucleosides will be discussed, viz., (i) a new strategy of the cascade one-pot transformation of D-pentoses into nucleosides based on the extension and deepening of the knowledge of the ...
A. Mikhailopulo, A. I. Miroshnikov
doaj   +1 more source