Results 61 to 70 of about 40,216 (223)

In Vitro Capacitation in Boar Sperm: Evaluation of Selected Detection Techniques

Methods and Protocols
Sperm capacitation is essential for fertilization and involves coordinated changes in membrane organization, ion fluxes, and intracellular signaling. However, commonly used detection methods may reflect different biological events, which can be strongly ...
Barbora Klusackova, Zuzana Pilsova, Katerina Nemeckova, Aneta Pilsova, Pavla Postlerova   +4 more
doaj   +1 more source

Phosphotyrosine Substrate Sequence Motifs for Dual Specificity Phosphatases. [PDF]

PLoS ONE, 2015
Protein tyrosine phosphatases dephosphorylate tyrosine residues of proteins, whereas, dual specificity phosphatases (DUSPs) are a subgroup of protein tyrosine phosphatases that dephosphorylate not only Tyr(P) residue, but also the Ser(P) and Thr(P ...
Bryan M Zhao, Sarah L Keasey, Joseph E Tropea, George T Lountos, Beverly K Dyas, Scott Cherry, Sreejith Raran-Kurussi, David S Waugh, Robert G Ulrich   +8 more
doaj   +1 more source

Muscle‐Specific Kinase Signaling and Its Therapeutic Potential

Muscle &Nerve, EarlyView.
ABSTRACT The function of the neuromuscular junction (NMJ) is compromised in many neuromuscular diseases (NMDs) such as autoimmune or congenital myasthenia gravis (MG), amyotrophic lateral sclerosis (ALS), spinal muscular atrophy (SMA), and muscular dystrophies.
Stine Marie Jensen, Dana L. E. Vergoossen, Maartje G. Huijbers   +2 more
wiley   +1 more source

A Remodelled Protease That Cleaves Phosphotyrosine Substrates [PDF]

Journal of the American Chemical Society, 2007
Proteases are the largest family of enzymes in the human genome and play central roles in a wide range of physiological processes.1 To carry out these diverse functions, proteolytic enzymes have evolved highly tuned substrate specificities that allow them to degrade specific cellular proteins in response to appropriate signals. For many proteases, this
Zachary A, Knight, Jennifer L, Garrison, Karina, Chan, David S, King, Kevan M, Shokat   +4 more
openaire   +2 more sources

Comparative Genomics and Functional Evidence for Convergent Adaptation to Hypoxia in Mammals

Integrative Zoology, EarlyView.
This study reveals convergent selection in mammals adapted to hypoxia. Functional assays demonstrate that amino acid mutations reduce oxidative stress, attenuate hypoxia‐induced injury, and modulate the hypoxia‐inducible transcription factor (HIF)‐driven hypoxic response.
Hao Dong, Chunxu Wang, Enqing Pei, Liang Zhao, Simin Chai, Ran Tian   +5 more
wiley   +1 more source

Identification of phosphotyrosine as a product of epidermal growth factor-activated protein kinase in A-431 cell membranes.

Journal of Biological Chemistry, 1980
Epidermal growth factor (EGF)-enhanced protein kinase activity in plasma membrane preparations of A-431 human epidermoid carcinoma cells was shown to involve the phosphorylation of tyrosine residues.
H. Ushiro, S. Cohen
semanticscholar   +1 more source

Afatinib Overcomes Osimertinib Resistance via Egfr V804F Mutation in a Syngeneic Egfr‐Mutant Lung Cancer Mouse Model

Cancer Science, EarlyView.
Using an immunocompetent mouse model of Egfr Ex19del‐driven lung cancer, we found that osimertinib‐resistant tumors acquired Egfr V804F, the murine counterpart of human EGFR V802F. Afatinib, but not osimertinib, suppressed phosphorylation of EGFR and overcame V804F‐mediated resistance, providing a preclinical rationale for biomarker‐guided therapy ...
Takaaki Tanaka, Masato Fujitani, Masataka Taoka, Iwao Shimomura, Sachi Okawa, Shunta Mori, Tadahiro Kuribayashi, Jun Nishimura, Tomoka Nishimura, Ayako Morita, Naofumi Hara, Kiichiro Ninomiya, Go Makimoto, Hisao Higo, Kammei Rai, Eiki Ichihara, Shuta Tomida, Katsuyuki Hotta, Yosuke Togashi, Yoshinobu Maeda, Katsuyuki Kiura, Ryohei Katayama, Kadoaki Ohashi   +22 more
wiley   +1 more source

Bacterial EPIYA/EPIYA-like effector motifs and host regulatory pY-SH2 logic: a sequence-informed three-layer convergence model

Frontiers in Cellular and Infection Microbiology
Bacterial pathogens frequently manipulate host signaling through short linear motifs (SLiMs) that are compact, rapidly evolvable, and often embedded in flexible effector regions. EPIYA/EPIYA-like effector motifs and related bacterial phosphotyrosine (pY)
Chunyan Wang, Congli Yuan
doaj   +1 more source

ALOX15 mediates thrombin‐induced myristoylation, trafficking, and interaction of protease‐activated receptors, leading to platelet activation and hemostasis

The FEBS Journal, EarlyView.
The role of PARs in platelet function is fairly known; however, the underlying mechanisms are not fully understood. We report that thrombin myristoylates PAR4 in mouse platelets and PAR1 and PAR4 in human platelets, facilitating their membrane transport and interaction, leading to PAR3/4 heterodimers in mice and PAR1/4 heterodimers in humans.
Suresh Govatati, Chandra Sekhar Yadavalli, Md Monirul Hoque, Swapna Thota, Gadiparthi N. Rao   +4 more
wiley   +1 more source

Dataset describing the development, optimization and application of SRM/MRM based targeted proteomics strategy for quantification of potential biomarkers of EGFR TKI sensitivity

Data in Brief, 2018
The data presented here describes the use of targeted proteomic assays to quantify potential biomarkers of Epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI) sensitivity in lung adenocarcinoma and is related to the research article: “
Shivangi Awasthi, Tapan Maity, Benjamin L. Oyler, Xu Zhang, David R. Goodlett, Udayan Guha   +5 more
doaj   +1 more source