Results 1 to 10 of about 45,758 (267)

Codon pair optimization (CPO): a software tool for synthetic gene design based on codon pair bias to improve the expression of recombinant proteins in Pichia pastoris

Microbial Cell Factories, 2021
Background Codon optimization is a common method to improve protein expression levels in Pichia pastoris and the current strategy is to replace rare codons with preferred codons to match the codon usage bias.
Yide Huang, Ting Lin, Lingfang Lu, Fan Cai, Jie Lin, Yu′e Jiang, Yao Lin   +6 more
doaj   +2 more sources

Advances in Metabolic Engineering of Pichia pastoris Strains as Powerful Cell Factories

Journal of Fungi, 2023
Pichia pastoris is the most widely used microorganism for the production of secreted industrial proteins and therapeutic proteins. Recently, this yeast has been repurposed as a cell factory for the production of chemicals and natural products.
Jian Zha, Dan Liu, Juan Ren, Zhijun Liu, Xia Wu   +4 more
doaj   +2 more sources

Current Understanding on the Heterogenous Expression of Plastic Depolymerising Enzymes in Pichia pastoris [PDF]

Bioengineering
Enzymatic depolymerisation is increasingly recognised as a reliable and environmentally friendly method. The development of this technology hinges on the availability of high-quality enzymes and associated bioreaction systems for upscaling biodegradation.
Shuyan Wu, David Hooks, Gale Brightwell
doaj   +2 more sources

Soft sensor modeling method for Pichia pastoris fermentation process based on substructure domain transfer learning [PDF]

BMC Biotechnology
Background Aiming at the problem that traditional transfer methods are prone to lose data information in the overall domain-level transfer, and it is difficult to achieve the perfect match between source and target domains, thus reducing the accuracy of ...
Bo Wang, Jun Wei, Le Zhang, Hui Jiang, Cheng Jin, Shaowen Huang   +5 more
doaj   +2 more sources

Industrial Production of Proteins with Pichia pastoris—Komagataella phaffii

Biomolecules, 2023
Since the mid-1960s, methylotrophic yeast Komagataella phaffii (previously described as Pichia pastoris) has received increasing scientific attention.
G. D. Barone, Anita Emmerstorfer-Augustin, A. Biundo, Isabella Pisano, P. Coccetti, Valeria Mapelli, Andrea Camattari   +6 more
semanticscholar   +1 more source

Cloning and Extracellular Expression of Glargine in Pichia pastoris

Hayati Journal of Biosciences, 2023
Patients with diabetes mellitus increase significantly every year. The increasing number of people with diabetes mellitus results in increased insulin requirements.
Dudi Hardianto, Efrida Martius, Tina Rostinawati, Anna Safarrida, Juwartina Ida Royani, Fahroziah Assyifa, Dihan Laziba   +6 more
doaj   +1 more source

Economical production of Pichia pastoris single cell protein from methanol at industrial pilot scale

Microbial Cell Factories, 2023
Background Methanol, synthesized from CO_2, is a potentially sustainable one-carbon (C1) resource for biomanufacturing. The use of methanol as a feedstock to produce single cell protein (SCP) has been investigated for decades as an alternative to ...
Jiao Meng, Shufan Liu, Le Gao, Kai Hong, Shuguang Liu, Xin Wu   +5 more
semanticscholar   +1 more source

Construction of recombinant Pichia pastoris strains for ammonia reduction by the gdhA and glnA regulatory genes in laying hens

Ecotoxicology and Environmental Safety, 2022
Ammonia emissions have become an important environmental challenge for the livestock industry. Probiotics are often used as additives to reduce ammonia, and the ammonia reduction efficiency of common probiotics is approximately 20–40%.
Kunxian Feng, Wei Wang, Jinsheng Rong, Juanboo Liang, Jiandui Mi, Yinbao Wu, Yan Wang   +6 more
doaj   +1 more source

Methanol biotransformation toward high-level production of fatty acid derivatives by engineering the industrial yeast Pichia pastoris

Proceedings of the National Academy of Sciences of the United States of America, 2022
Significance Methanol-based biomanufacturing is considered a promising way to achieve carbon neutrality. However, efficient methanol biotransformation toward chemical production is challenging.
Peng Cai, Xiaoyan Wu, Jun Deng, Linhui Gao, Yiwei Shen, Lun Yao, Yongjin J. Zhou   +6 more
semanticscholar   +1 more source

High-level expression of endo-β-N-acetylglucosaminidase H from Streptomyces plicatus in Pichia pastoris and its application for the deglycosylation of glycoproteins. [PDF]

PLoS ONE, 2015
Endo-β-N-acetylglucosaminidase H (Endo H, EC3.2.1.96) is a glycohydrolase that is widely used in the study of glycoproteins. The present study aimed to assess the effect of high-level endo-β-N-acetylglucosaminidase H expression in Pichia pastoris.
Fei Wang, Xiaojuan Wang, Xiaolan Yu, Ling Fu, Yunyun Liu, Lixin Ma, Chao Zhai   +6 more
doaj   +1 more source