Results 1 to 10 of about 24,148 (162)

Expression of lignocellulolytic enzymes in Pichia pastoris [PDF]

Microbial Cell Factories, 2012
Background Sustainable utilization of plant biomass as renewable source for fuels and chemical building blocks requires a complex mixture of diverse enzymes, including hydrolases which comprise the largest class of lignocellulolytic enzymes.
Mellitzer Andrea, Weis Roland, Glieder Anton, Flicker Karlheinz   +3 more
doaj   +8 more sources

Cloning and Extracellular Expression of Glargine in Pichia pastoris

Hayati Journal of Biosciences, 2023
Patients with diabetes mellitus increase significantly every year. The increasing number of people with diabetes mellitus results in increased insulin requirements.
Dudi Hardianto, Efrida Martius, Tina Rostinawati, Anna Safarrida, Juwartina Ida Royani, Fahroziah Assyifa, Dihan Laziba   +6 more
doaj   +1 more source

Construction of recombinant Pichia pastoris strains for ammonia reduction by the gdhA and glnA regulatory genes in laying hens

Ecotoxicology and Environmental Safety, 2022
Ammonia emissions have become an important environmental challenge for the livestock industry. Probiotics are often used as additives to reduce ammonia, and the ammonia reduction efficiency of common probiotics is approximately 20–40%.
Kunxian Feng, Wei Wang, Jinsheng Rong, Juanboo Liang, Jiandui Mi, Yinbao Wu, Yan Wang   +6 more
doaj   +1 more source

High-level expression of endo-β-N-acetylglucosaminidase H from Streptomyces plicatus in Pichia pastoris and its application for the deglycosylation of glycoproteins. [PDF]

PLoS ONE, 2015
Endo-β-N-acetylglucosaminidase H (Endo H, EC3.2.1.96) is a glycohydrolase that is widely used in the study of glycoproteins. The present study aimed to assess the effect of high-level endo-β-N-acetylglucosaminidase H expression in Pichia pastoris.
Fei Wang, Xiaojuan Wang, Xiaolan Yu, Ling Fu, Yunyun Liu, Lixin Ma, Chao Zhai   +6 more
doaj   +1 more source

Codon pair optimization (CPO): a software tool for synthetic gene design based on codon pair bias to improve the expression of recombinant proteins in Pichia pastoris

Microbial Cell Factories, 2021
Background Codon optimization is a common method to improve protein expression levels in Pichia pastoris and the current strategy is to replace rare codons with preferred codons to match the codon usage bias.
Yide Huang, Ting Lin, Lingfang Lu, Fan Cai, Jie Lin, Yu′e Jiang, Yao Lin   +6 more
doaj   +1 more source

PERSPECTIVE DE PRODUCERE A HORMONULUI BIOSIMILAR DE CREȘTERE UMAN RECOMBINAT ÎN REPUBLICA MOLDOVA

Buletinul Academiei de Ştiinţe a Moldovei: Ştiinţe Medicale, 2022
În cadrul colaborării între USMF «N.Testemițanu», întreprinderea farmaceutică Balkan Pharmaceuticals și ICGEB a fost efectuat transferul tehnologic la etapa extrapolării de la faza de laborator la faza pilot a proceselor creării băncii celulelor de lucru
Liliana RUSNAC, Radu CAZACU, Mihail TODIRAȘ, Corina SCUTARI, Vladimir VALICA   +4 more
doaj   +1 more source

Evaluation of Toxocara canis Glycosylated TES Produced in Pichia pastoris for Immunodiagnosis of Human Toxocariasis [PDF]

Brazilian Archives of Biology and Technology, 2020
Recombinant proteins are a suggested alternative for the diagnosis of toxocariasis. The current Escherichia coli recombinant protein overexpression system usually produces insoluble products.
Lucas Moreira dos Santos, Michele Pepe Cerqueira, Giana Carla Gaboardi, Carolina Georg Magalhães, Rafael Amaral Donassolo, Rafael Rodrigues Rodrigues, Emili Griep, Marcos Roberto Ferreira, Guita Rubinsky Elefant, Luciana Farias da Costa Avila, Carlos James Scaini, Ângela Nunes Moreira, Fabricio Rochedo Conceição   +12 more
doaj   +1 more source

A multi-model predictive control method for the Pichia pastoris fermentation process based on relative error weighting algorithm

Alexandria Engineering Journal, 2022
The Pichia pastoris fermentation process is with highly nonlinear and time-varying and strong coupling characteristics, traditional control methods such as classic PID cannot satisfy the actual needs of the fermentation process control.
Bo Wang, Mengyi He, Xingyu Wang, Hongyu Tang, Xianglin Zhu   +4 more
doaj   +1 more source

Co-expressing GroEL–GroES, Ssa1–Sis1 and Bip–PDI chaperones for enhanced intracellular production and partial-wall breaking improved stability of porcine growth hormone

Microbial Cell Factories, 2020
Porcine growth hormone (pGH) is a class of peptide hormones secreted from the pituitary gland, which can significantly improve growth and feed utilization of pigs. However, it is unstable and volatile in vitro.
Jinbo Deng, Jiaoqing Li, Miaopeng Ma, Peijing Zhao, Feiping Ming, Zhipeng Lu, Juqing Shi, Qin Fan, Qianyi Liang, Junhao Jia, Jiayi Li, Shuxia Zhang, Linghua Zhang   +12 more
doaj   +1 more source

Development and Optimization of a Novel Soft Sensor Modeling Method for Fermentation Process of Pichia pastoris

Sensors, 2023
This paper introduces a novel soft sensor modeling method based on BDA-IPSO-LSSVM designed to address the issue of model failure caused by varying fermentation data distributions resulting from different operating conditions during the fermentation of ...
Bo Wang, Jun Liu, Ameng Yu, Haibo Wang
doaj   +1 more source