Results 91 to 100 of about 44,813 (273)
Pichia pastoris ‘just in time’ alternative respiration [PDF]
Alternative oxidases (Aox or Aod) are present in the mitochondria of plants, fungi and many types of yeast. These enzymes transfer electrons from the ubiquinol pool directly to oxygen without contributing to the proton transfer across the mitochondrial membrane.
Alexander, Kern +7 more
openaire +2 more sources
Liquid PTVA: A faster and cheaper alternative for generating multi-copy clones in Pichia pastoris [PDF]
Additional file 1: Figure S1.
Aw, R, Polizzi, KM
core +5 more sources
ABSTRACT After an initial evolution in a reducing environment, life got successively challenged by reactive oxygen species (ROS), especially during the great oxidation event (GOE) that followed the development of photosynthesis. Therefore, ROS are deeply intertwined into the physiological, morphological and transcriptional responses of most present‐day
Stephanie Frohn +12 more
wiley +1 more source
Phytase gene obtained from Bacillus subtilis ASUIA243 was cloned into a medium vector and transformed into E. coli. Restriction enzyme digestion was conducted to get blunt-ended phytase gene and ligated into the Pichia expression vector, pPICZαA.
Nor Soleha Mohd Dali +6 more
doaj +1 more source
Pay32p of the Yeast Yarrowia lipolytica Is an Intraperoxisomal Component of the Matrix Protein Translocation Machinery [PDF]
Pay mutants of the yeast Yarrowia lipolytica fail to assemble functional peroxisomes. One mutant strain, pay32-1, has abnormally small peroxisomes that are often found in clusters surrounded by membranous material.
Rachubinski, Richard A., +3 more
core +3 more sources
Exploring the Controllability of Microbial Metabolism on the Flavor of Chinese Baijiu
Regulation of flavor compounds by core functional microorganisms. ABSTRACT The flavor and quality of Baijiu are closely related to the environment and microbial metabolism. The microbial community structure is highly dynamic in time and space, making it trouble to control the flavor and quality of Baijiu. Therefore, this paper reviewed the role of core
Hang Ying +6 more
wiley +1 more source
Pediocin (Ped) is a 4.6kDa bacteriocin effective against Listeria monocytogenes and several Gram-positive bacteria. To produce purified Ped with high yield and apply it to food preservation, this study expresses and purifies recombinant Ped (rPed) from ...
Nguyễn Phạm Anh Thư +2 more
doaj +1 more source
This data article refers to the report Δ9-Tetrahydrocannabinolic acid synthase (THCAS) production in Pichia pastoris enables chemical synthesis of cannabinoids (Lange et. al. 2015) [2]. THCAS was produced on a 2 L lab scale using recombinant P.
Kerstin Lange +3 more
doaj +1 more source
Automated pipeline for rapid production and screening of HIV-specific monoclonal antibodies using pichia pastoris [PDF]
Monoclonal antibodies (mAbs) that bind and neutralize human pathogens have great therapeutic potential. Advances in automated screening and liquid handling have resulted in the ability to discover antigen-specific antibodies either directly from human ...
Clark, John J +8 more
core +1 more source
ABSTRACT Expansins loosen plant cell wall networks through disrupting non‐covalent bonds between cellulose microfibrils and matrix polysaccharides. Whereas expansins were first discovered in plants, expansin‐related proteins have since been identified in bacteria and fungi.
Anna Pohto +8 more
wiley +1 more source

