Results 91 to 100 of about 45,758 (267)
Pest Management Science, EarlyView.Plants possess an innate immune system able to detect pathogens' molecules such as cerato‐platanin (CP), a protein produced by the fungus Ceratocystis platani. This study shows that the application of this protein elicitor to weeds can be exploited as a novel herbicide mode of action.
Laura Scarabel +5 more
wiley +1 more source
Competition between binding partners of yeast Pex3 affects peroxisome biology
The FEBS Journal, EarlyView.Pex3 is a peroxisomal membrane protein (PMP) that specifically recruits several binding partners. In the yeast Hansenula polymorpha, Atg30 (pexophagy), Inp1 (inheritance) and Pex19 (receptor for new PMPs) associate with Pex3. Overexpression of any of these proteins affects peroxisomal processes because these proteins compete for binding to Pex3.
Eline M. F. de Lange +5 more
wiley +1 more source
Synthetic Core Promoters for Pichia pastoris
ACS Synthetic Biology, 2013 Synthetic promoters are commonly used tools for circuit design or high level protein production. Promoter engineering efforts in yeasts, such as Saccharomyces cerevisiae and Pichia pastoris have mostly been focused on altering upstream regulatory sequences such as transcription factor binding sites.
Thomas Kickenweiz +4 more
openaire +3 more sources
Microbiology Indonesia, 2017 Lipases (EC 3.1.1.3) are classified as hydrolases that hydrolyze lipids. These enzymes have potential application in biotechnology and industrial process. In previous study we have cloned the synthetic Rhizomucor miehei lipase gene using the vector pUC57
doaj +1 more source
Citrobacter amalonaticus phytase on the cell surface of Pichia pastoris exhibits high pH stability as a promising potential feed supplement. [PDF]
PLoS ONE, 2014 Phytase expressed and anchored on the cell surface of Pichia pastoris avoids the expensive and time-consuming steps of protein purification and separation. Furthermore, yeast cells with anchored phytase can be used as a whole-cell biocatalyst.
Cheng Li +4 more
doaj +1 more source
Plant Biotechnology Journal, EarlyView.Summary Antifungal proteins (AFPs) derived from filamentous fungi show great potential against economically significant fungi that cause plant diseases and consequently threat food safety and security. This study focuses on the Penicillium expansum PeAfpA and Penicillium digitatum PdAfpB proteins and their activity against several phytopathogens.
Adrià Bugeda +6 more
wiley +1 more source
Plant, Cell &Environment, EarlyView.ABSTRACT After an initial evolution in a reducing environment, life got successively challenged by reactive oxygen species (ROS), especially during the great oxidation event (GOE) that followed the development of photosynthesis. Therefore, ROS are deeply intertwined into the physiological, morphological and transcriptional responses of most present‐day
Stephanie Frohn +12 more
wiley +1 more source
International Islamic University Malaysia Engineering Journal, 2011 Phytase gene obtained from Bacillus subtilis ASUIA243 was cloned into a medium vector and transformed into E. coli. Restriction enzyme digestion was conducted to get blunt-ended phytase gene and ligated into the Pichia expression vector, pPICZαA.
Nor Soleha Mohd Dali +6 more
doaj +1 more source
IRES-mediated Pichia pastoris cell-free protein synthesis
Bioresources and Bioprocessing, 2023 Cell-free protein synthesis (CFPS) system is an ideal platform for fast and convenient protein research and has been used for macromolecular assembly, unnatural amino acid embedding, glycoprotein production, and more.
Yanan Wang +3 more
doaj +1 more source
Biotechnology and Bioengineering, Volume 122, Issue 9, Page 2478-2498, September 2025.ABSTRACT Komagataella phaffii, also known as Pichia pastoris, is a powerful host for recombinant protein production, in part due to its exceptionally strong and tightly controlled PAOX1 promoter. Most K. phaffii bioprocesses for recombinant protein production rely on PAOX1 to achieve dynamic control in two‐phase processes.
Shannon M. Hoffman +4 more
wiley +1 more source