Results 11 to 20 of about 45,758 (267)
Expression of lignocellulolytic enzymes in
Microbial Cell Factories, 2012 Background Sustainable utilization of plant biomass as renewable source for fuels and chemical building blocks requires a complex mixture of diverse enzymes, including hydrolases which comprise the largest class of lignocellulolytic enzymes.
Mellitzer Andrea +3 more
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Current advances of Pichia pastoris as cell factories for production of recombinant proteins
Frontiers in Microbiology, 2022 Pichia pastoris (syn. Komagataella spp.) has attracted extensive attention as an efficient platform for recombinant protein (RP) production. For obtaining a higher protein titer, many researchers have put lots of effort into different areas and made some
Ying-jie Pan +4 more
semanticscholar +1 more source
ACS Synthetic Biology, 2022 Bioactive plant-based secondary metabolites such as stilbenoids, flavonoids, and benzylisoquinoline alkaloids (BIAs) are produced from l-tyrosine (l-Tyr) and have a wide variety of commercial applications.
Ryota Kumokita +6 more
semanticscholar +1 more source
PERSPECTIVE DE PRODUCERE A HORMONULUI BIOSIMILAR DE CREȘTERE UMAN RECOMBINAT ÎN REPUBLICA MOLDOVA
Buletinul Academiei de Ştiinţe a Moldovei: Ştiinţe Medicale, 2022 În cadrul colaborării între USMF «N.Testemițanu», întreprinderea farmaceutică Balkan Pharmaceuticals și ICGEB a fost efectuat transferul tehnologic la etapa extrapolării de la faza de laborator la faza pilot a proceselor creării băncii celulelor de lucru
Liliana RUSNAC +4 more
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Nucleic Acids Research, 2021 The industrial yeast Pichia pastoris has been harnessed extensively for production of proteins, and it is attracting attention as a chassis cell factory for production of chemicals.
Peng Cai +5 more
semanticscholar +1 more source
Evaluation of Toxocara canis Glycosylated TES Produced in Pichia pastoris for Immunodiagnosis of Human Toxocariasis [PDF]
Brazilian Archives of Biology and Technology, 2020 Recombinant proteins are a suggested alternative for the diagnosis of toxocariasis. The current Escherichia coli recombinant protein overexpression system usually produces insoluble products.
Lucas Moreira dos Santos +12 more
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Development of quantitative metabolomics for Pichia pastoris [PDF]
Metabolomics, 2011 Accurate, reliable and reproducible measurement of intracellular metabolite levels has become important for metabolic studies of microbial cell factories. A first critical step for metabolomic studies is the establishment of an adequate quenching and washing protocol, which ensures effective arrest of all metabolic activity and removal of extracellular
Carnicer,, M. (author) +8 more
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An improved CRISPRi system in Pichia pastoris
Synthetic and Systems Biotechnology, 2023 CRISPR interference (CRISPRi) has been developed and widely used for gene repression in various hosts. Here we report an improved CRISPRi system in Pichia pastoris by fusing dCas9 with endogenous transcriptional repressor domains. The CRISPRi system shows strong repression of eGFP, with the highest efficiency of 85%.
Shujing Qiao +4 more
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Second generation Pichia pastoris strain and bioprocess designs
Biotechnology for Biofuels and Bioproducts, 2022 Yeast was the first microorganism used by mankind for biotransformation processes that laid the foundations of industrial biotechnology. In the last decade, Pichia pastoris has become the leading eukaryotic host organism for bioproduct generation.
Burcu Gündüz Ergün +3 more
semanticscholar +1 more source
Alexandria Engineering Journal, 2022 The Pichia pastoris fermentation process is with highly nonlinear and time-varying and strong coupling characteristics, traditional control methods such as classic PID cannot satisfy the actual needs of the fermentation process control.
Bo Wang +4 more
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