Results 11 to 20 of about 44,813 (273)

Gonadotropins in the Russian Sturgeon: Their Role in Steroid Secretion and the Effect of Hormonal Treatment on Their Secretion. [PDF]

open access: gold, 2016
In the reproduction process of male and female fish, pituitary derived gonadotropins (GTHs) play a key role. To be able to specifically investigate certain functions of Luteinizing (LH) and Follicle stimulating hormone (FSH) in Russian sturgeon ...
Aizen, Joseph   +8 more
core   +10 more sources

Co-expressing GroEL–GroES, Ssa1–Sis1 and Bip–PDI chaperones for enhanced intracellular production and partial-wall breaking improved stability of porcine growth hormone

open access: yesMicrobial Cell Factories, 2020
Porcine growth hormone (pGH) is a class of peptide hormones secreted from the pituitary gland, which can significantly improve growth and feed utilization of pigs. However, it is unstable and volatile in vitro.
Jinbo Deng   +12 more
doaj   +1 more source

Development and Optimization of a Novel Soft Sensor Modeling Method for Fermentation Process of Pichia pastoris

open access: yesSensors, 2023
This paper introduces a novel soft sensor modeling method based on BDA-IPSO-LSSVM designed to address the issue of model failure caused by varying fermentation data distributions resulting from different operating conditions during the fermentation of ...
Bo Wang, Jun Liu, Ameng Yu, Haibo Wang
doaj   +1 more source

Cloning and Expression of Yak Active Chymosin in [PDF]

open access: yesAsian-Australasian Journal of Animal Sciences, 2016
Rennet, a complex of enzymes found in the stomachs of ruminants, is an important component for cheese production. In our study, we described that yak chymosin gene recombinant Pichia pastoris strain could serve as a novel source for rennet production ...
Fan Luo   +4 more
doaj   +1 more source

Production of a soluble and functional recombinant apolipoproteinD in the Pichia pastoris expression system [PDF]

open access: yes, 2016
ApolipoproteinD (ApoD) is a human glycoprotein from the lipocalin family. ApoD contains a conserved central motif of an 8-stranded antiparallel β-sheet, which forms a beta-barrel that can be used for transport and storage of diverse hydrophobic ligands ...
Armanmehr, S.   +2 more
core   +1 more source

High-yield overproduction and purification of human aquaporins from Pichia pastoris

open access: yesSTAR Protocols, 2022
Summary: Aquaporins (AQPs) are membrane-bound water channels that play crucial roles in maintaining the water homeostasis of the human body. Here, we present a protocol for high-yield recombinant expression of human AQPs in the methylotropic yeast Pichia
Tamim Al-Jubair   +7 more
doaj   +1 more source

Mutational analysis of the carbohydrate binding activity of the tobacco lectin [PDF]

open access: yes, 2010
At present the three-dimensional structure of the tobacco lectin, further referred to as Nictaba, and its carbohydrate-binding site are unresolved. In this paper, we propose a three-dimensional model for the Nictaba domain based on the homology between ...
Barre, Annick   +6 more
core   +2 more sources

Pichia pastoris Fep1 is a [2Fe-2S] protein with a Zn finger that displays an unusual oxygen-dependent role in cluster binding [PDF]

open access: yes, 2016
Fep1, the iron-responsive GATA factor from the methylotrophic yeast Pichia pastoris, has been characterised both in vivo and in vitro. This protein has two Cys(2)-Cys(2) type zinc fingers and a set of four conserved cysteines arranged in a Cys-X-5-Cys-X ...
Battistoni, Andrea   +8 more
core   +3 more sources

Refined Pichia pastoris reference genome sequence [PDF]

open access: yesJournal of Biotechnology, 2016
Strains of the species Komagataella phaffii are the most frequently used "Pichia pastoris" strains employed for recombinant protein production as well as studies on peroxisome biogenesis, autophagy and secretory pathway analyses. Genome sequencing of several different P.
Sturmberger   +17 more
openaire   +2 more sources

Efficient display of active lipase LipB52 with a Pichia pastoris cell surface display system and comparison with the LipB52 displayed on Saccharomyces cerevisiae cell surface

open access: yesBMC Biotechnology, 2008
Background For industrial bioconversion processes, the utilization of surface-displayed lipase in the form of whole-cell biocatalysts is more advantageous, because the enzymes are displayed on the cell surface spontaneously, regarded as immobilized ...
Ma Yushu   +5 more
doaj   +1 more source

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