Results 31 to 40 of about 45,758 (267)
The effect of hypoxia on the lipidome of recombinant Pichia pastoris [PDF]
Microbial Cell Factories, 2017 Cultivation of recombinant Pichia pastoris (Komagataella sp.) under hypoxic conditions has a strong positive effect on specific productivity when the glycolytic GAP promoter is used for recombinant protein expression, mainly due to upregulation of glycolytic conditions. In addition, transcriptomic analyses of hypoxic P.
Núria Adelantado +8 more
openaire +6 more sources
Codon optimization significantly improves the expression level of a keratinase gene in Pichia pastoris. [PDF]
PLoS ONE, 2013 The main keratinase (kerA) gene from the Bacillus licheniformis S90 was optimized by two codon optimization strategies and expressed in Pichia pastoris in order to improve the enzyme production compared to the preparations with the native kerA gene.
Hong Hu +9 more
doaj +1 more source
Bioengineering, 2021 Vaccination is of paramount importance to global health. With the advent of the more recent pandemics, the urgency to expand the range has become even more evident. However, the potential limited availability and affordability of vaccines to resource low-
Salomé de Sá Magalhães +1 more
semanticscholar +1 more source
Screening and evaluation of the strong endogenous promoters in Pichia pastoris
Microbial Cell Factories, 2021 Background Due to its ability to perform fast and high-density fermentation, Pichia pastoris is not only used as an excellent host for heterologous protein expression but also exhibits good potential for efficient biosynthesis of small-molecule compounds.
Weiwang Dou +4 more
semanticscholar +1 more source
Metabolic engineering of Pichia pastoris
Metabolic Engineering, 2018 Besides its use for efficient production of recombinant proteins the methylotrophic yeast Pichia pastoris (syn. Komagataella spp.) has been increasingly employed as a platform to produce metabolites of varying origin. We summarize here the impressive methodological developments of the last years to model and analyze the metabolism of P.
David A. Peña +4 more
openaire +3 more sources
Overexpression of alcohol oxidase in Pichia pastoris [PDF]
FEBS Letters, 1991 The protein import capacity of peroxisomes in methylotrophic yeasts was studied using Pichia pastoris containing one or two extra copies of the gene encoding the peroxisomal protein alcohol oxidase. The alcohol oxidase overproduced in this strain was only partially imported and assembled into the active, octameric form of the protein.
James M. Cregg +5 more
openaire +2 more sources
Improved Production of Recombinant Myrosinase in Pichia pastoris [PDF]
International Journal of Molecular Sciences, 2021 The effect of the deletion of a 57 bp native signal sequence, which transports the nascent protein through the endoplasmic reticulum membrane in plants, on improved AtTGG1 plant myrosinase production in Pichia pastoris was studied. Myrosinase was extracellularly produced in a 3-liter laboratory fermenter using α-mating factor as the secretion signal ...
Zuzana Rosenbergová +4 more
openaire +3 more sources
Comparative proteomics analysis of Pichia pastoris cultivating in glucose and methanol
Synthetic and Systems Biotechnology, 2022 The methylotrophic yeast Pichia pastoris (syn. Komagataella phaffii) has been extensively engineered for protein production, and is attracting attention as a chassis cell for methanol biotransformation toward production of small molecules.
Rui Hou +6 more
doaj +1 more source
AMB Express, 2021 The negative effects of lignocellulose-derived inhibitors such as acetic acid and furaldehydes on microbial metabolism constitute a significant drawback to the usage of biomass feedstocks for the production of fuels and chemicals.
B. G. Paes +4 more
semanticscholar +1 more source
Scientific Reports, 2020 The yeast Pichia pastoris is a cost-effective and easily scalable system for recombinant protein production. In this work we compared the conformation of the receptor binding domain (RBD) from severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2 ...
C. R. Arbeitman +29 more
semanticscholar +1 more source