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Preparation of Pichia pastoris Expression Plasmids [PDF]
, 2012 When planning any heterologous expression experiment, the very first critical step is related to the design of the overall strategy, hence to the selection of the most adapted expression vector. The very flexible Pichia pastoris system offers a broad range of possibilities for the production of secreted, endogenous or membrane proteins thanks to a ...
Logez, C +3 more
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Trends in Peptide and Protein Sciences, 2017 The use of the methylotrophic yeast, Pichia pastoris, as one of the most effective and versatile systems for the expression of heterologous proteins in biopharmaceutical manufacturing has become increasingly popular in recent years.
Atefeh Namipashaki +3 more
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Benchmarking recombinant Pichia pastoris for 3‐hydroxypropionic acid production from glycerol
Microbial Biotechnology, 2021 The use of the methylotrophic yeast Pichia pastoris (Komagataella phaffi) to produce heterologous proteins has been largely reported. However, investigations addressing the potential of this yeast to produce bulk chemicals are still scarce. In this study,
A. Fina +6 more
semanticscholar +1 more source
CHIMIA, 2005 During our work, flow cytometry was used for a study of the influence of cultivation conditions on a physiological state, mainly on the viability and vitality of the recombinant Pichia pastoris strain producing ?-galactosidase.
Petr Hyka +3 more
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Increasing the production of insulin glargine in Pichia pastoris through medium modification [PDF]
BIO Web of ConferencesInsulin is the primary drug for managing diabetes mellitus, especially for individuals with type 1 diabetes mellitus. Producing insulin glargine, a long-acting insulin analogue, using Pichia pastoris is a notable advancement in biopharmaceutical ...
Hardianto Dudi +7 more
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Microbial Cell Factories, 2023 Background Recently, researchers have focused on the search for alternatives to conventional antibiotics. Antimicrobial peptides are small bioactive peptides that regulate immune activation and have antibacterial activity with a reduced risk of bacterial
Chunming Dong +7 more
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Metabolic engineering of methylotrophic Pichia pastoris for the production of β-alanine
Bioresources and Bioprocessing, 2021 β-Alanine (3-aminopropionic acid) is the only naturally occurring β-amino acid and an important precursor for the synthesis of a variety of nitrogen-containing chemicals.
L. Miao, Yin Li, T. Zhu
semanticscholar +1 more source
Oxidative stress tolerance contributes to heterologous protein production in Pichia pastoris
Biotechnology for Biofuels, 2021 Background Pichia pastoris (syn. Komagataella phaffii ) is an important yeast system for heterologous protein expression. A robust P. pastoris mutant with oxidative and thermal stress cross-tolerance was acquired in our previous study.
N. Lin, R. He, Yan Xu, Xiaowei Yu
semanticscholar +1 more source
Synthesis of polyhydroxyalkanoate in the peroxisome ofPichia pastoris [PDF]
FEMS Microbiology Letters, 2002 Polyhydroxyalkanoates (PHAs) are polyesters naturally produced by bacteria that have properties of biodegradable plastics and elastomers. A PHA synthase from Pseudomonas aeruginosa modified at the carboxy-end for peroxisomal targeting was transformed in Pichia pastoris. The PHA synthase was expressed under the control of the promoter of the P. pastoris
Poirier, Yves +2 more
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Synthetic and Systems Biotechnology, 2021 The methylotrophic yeast Pichia pastoris (a.k.a. Komagataella phaffii) is one of the most commonly used hosts for industrial production of recombinant proteins. As a non-conventional yeast, P.
Jucan Gao, Lihong Jiang, Jiazhang Lian
doaj +1 more source