Results 41 to 50 of about 44,813 (273)
Synthetic and Systems Biotechnology, 2021 The methylotrophic yeast Pichia pastoris (a.k.a. Komagataella phaffii) is one of the most commonly used hosts for industrial production of recombinant proteins. As a non-conventional yeast, P.
Jucan Gao, Lihong Jiang, Jiazhang Lian
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Expression of Aspergillus niger glucose oxidase in Pichia pastoris and its antimicrobial activity against Agrobacterium and Escherichia coli [PDF]
PeerJ, 2020 The gene encoding glucose oxidase from Aspergillus niger ZM-8 was cloned and transferred to Pichia pastoris GS115, a transgenic strain P. pastoris GS115-His-GOD constructed. The growth curve of P. pastoris GS115-His-GOD was consistent with that of Pichia
Yonggang Wang +4 more
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Comparison of CRISPR-MAD7 and CRISPR-Cas9 for Gene Disruptions in Komagataella phaffii
Journal of FungiCRISPR (clustered regularly interspaced short palindromic repeats)-based technologies are powerful, programmable tools for site-directed genome modifications.
Kirill Smirnov +6 more
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Microbial Cell Factories, 2023 Background Ablynx NV, a subsidiary of Sanofi, has a long-standing focus on the development of Nanobody® molecules as biopharmaceuticals (Nanobody® is a registered trademark of Ablynx NV). Nanobody molecules are single variable domains, and they have been
Manu De Groeve +2 more
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, 2018 Yeasts are a group of eukaryotic microfungi with a well-defined cell wall whose growth is either entirely unicellular or a combination of hyphal and unicellular reproduction. The approximately 1500 known yeast species belong to two distinct fungal phyla,
Lachance, Marc-Andre, Walker, Graeme M.
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Usos de la levadura Pichia pastoris en la producción de proteínas recombinantes
VacciMonitor, 2021 La levadura metilotrófica Pichia pastoris (clasificada actualmente como Komagataella phaffii) es una de las más importantes para la producción de proteínas heterólogas. En el trabajo se presenta un análisis de las principales características que se ponen
José García-Suárez +2 more
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Microbial Cell Factories / Methanol regulated yeast promoters: production vehicles and toolbox for synthetic biology [PDF]
, 2015 Promoters are indispensable elements of a standardized parts collection for synthetic biology. Regulated promoters of a wide variety of well-defined induction ratios and expression strengths are highly interesting for many applications.
Gasser, Brigitte +2 more
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Recombinant production of bacterial toxins and their derivatives in the methylotrophic yeast Pichia pastoris. [PDF]
, 2005 RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'.
Ellar, David J, Gurkan, Cemal
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Microbial Cell FactoriesBackground Most recombinant Komagataella phaffii (Pichia pastoris) strains for protein production are generated by genomic integration of expression cassettes.
Florian Weiss +5 more
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A Study and Application of Biocatalytic Synthesis of (S)-N-Boc-3-hydroxypiperidine
MATEC Web of Conferences, 2015 This paper first uses the environmental friendly whole cell of biocatalyst pichia pastoris SIT2014 to asymmetrically synthesized anti-tumor drug of chiral intermediate (S)-N-Boc-3-hydroxypiperidine.. Improve the final biocatalytic reduction yield to 85.4%
Chen Ying, Xu Yi, Chen Jianbo
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