Results 51 to 60 of about 45,758 (267)

Improving the activity and thermostability of PETase from Ideonella sakaiensis through modulating its post-translational glycan modification

Communications Biology, 2023
Polyehylene terephthalate (PET) biodegradation is greatly improved when recombinant IsPETase is expressed in Pichia pastoris and the enzyme’s partial removal of N-terminal glycosylation, retaining high thermostability.
Binyang Deng, Yu Yue, Jun Yang, Mingjun Yang, Qiong Xing, Hang Peng, Fei Wang, Ming Li, Lixin Ma, Chao Zhai   +9 more
doaj   +1 more source

Expression of Aspergillus niger glucose oxidase in Pichia pastoris and its antimicrobial activity against Agrobacterium and Escherichia coli [PDF]

PeerJ, 2020
The gene encoding glucose oxidase from Aspergillus niger ZM-8 was cloned and transferred to Pichia pastoris GS115, a transgenic strain P. pastoris GS115-His-GOD constructed. The growth curve of P. pastoris GS115-His-GOD was consistent with that of Pichia
Yonggang Wang, Jiangqin Wang, Feifan Leng, Jianzhong Ma, Alnoor Bagadi   +4 more
doaj   +2 more sources

Established tools and emerging trends for the production of recombinant proteins and metabolites in Pichia pastoris.

Essays in Biochemistry, 2021
Besides bakers' yeast, the methylotrophic yeast Komagataella phaffii (also known as Pichia pastoris) has been developed into the most popular yeast cell factory for the production of heterologous proteins.
Sonakshi De, D. Mattanovich, P. Ferrer, Brigitte Gasser   +3 more
semanticscholar   +1 more source

Genotyping of recombinant Pichia pastoris strains [PDF]

Cellular and Molecular Biology Letters, 2006
AbstractA simplified amplified-fragment length polymorphism (AFLP) method was used to genotype Pichia pastoris strains obtained by transformation of P. pastoris strain GS115 with a single integration vector. A total of 14 transformants and 3 control strains were analyzed, which generated 16 different band patterns. A clonal variation was obtained after
Viader Salvadó, José María, Cab Barrera, Eddy Luz, Galán Wong, Luis Jesús, Guerrero Olazarán, Martha   +3 more
openaire   +4 more sources

Optimizing expression of Nanobody® molecules in Pichia pastoris through co-expression of auxiliary proteins under methanol and methanol-free conditions

Microbial Cell Factories, 2023
Background Ablynx NV, a subsidiary of Sanofi, has a long-standing focus on the development of Nanobody® molecules as biopharmaceuticals (Nanobody® is a registered trademark of Ablynx NV). Nanobody molecules are single variable domains, and they have been
Manu De Groeve, Bram Laukens, Peter Schotte   +2 more
doaj   +1 more source

Constitutive expression of Camelus bactrianus prochymosin B in Pichia pastoris

Heliyon, 2021
Camel chymosin can be efficiently employed to produce cheese. Traditionally the rennet enzyme produced by the glands of the fourth stomach of ruminant animals (abomassum) is used in cheese making.
Zhiger Akishev, Assel Kiribayeva, Arman Mussakhmetov, Kairat Baltin, Yerlan Ramankulov, Bekbolat Khassenov   +5 more
doaj   +1 more source

Dynamics in bioprocess development forPichia pastoris [PDF]

Bioengineered, 2014
Pichia pastoris is a widely used host organism for the recombinant production of proteins. It is attracting increasing interest for the production of biopharmaceuticals, due to its capability of performing posttranslational modifications. Traditionally, production processes with P.
Oliver Spadiut, Christoph Herwig
openaire   +3 more sources

Comparison of CRISPR-MAD7 and CRISPR-Cas9 for Gene Disruptions in Komagataella phaffii

Journal of Fungi
CRISPR (clustered regularly interspaced short palindromic repeats)-based technologies are powerful, programmable tools for site-directed genome modifications.
Kirill Smirnov, Florian Weiss, Anna-Maria Hatzl, Lukas Rieder, Kjeld Olesen, Sanne Jensen, Anton Glieder   +6 more
doaj   +1 more source

Usos de la levadura Pichia pastoris en la producción de proteínas recombinantes

VacciMonitor, 2021
La levadura metilotrófica Pichia pastoris (clasificada actualmente como Komagataella phaffii) es una de las más importantes para la producción de proteínas heterólogas. En el trabajo se presenta un análisis de las principales características que se ponen
José García-Suárez, Lourdes Zumalacárregui-de-Cárdenas, Zeila Santana-Vázquez   +2 more
doaj  

Exchange of endogenous and heterogeneous yeast terminators in Pichia pastoris to tune mRNA stability and gene expression

Nucleic Acids Research, 2020
In the yeast Saccharomyces cerevisiae, terminator sequences not only terminate transcription but also affect expression levels of the protein-encoded upstream of the terminator. The non-conventional yeast Pichia pastoris (syn.
Y. Ito, G. Terai, M. Ishigami, Noriko Hashiba, Yasuyuki Nakamura, Takahiro Bamba, Ryota Kumokita, T. Hasunuma, K. Asai, Jun Ishii, A. Kondo   +10 more
semanticscholar   +1 more source