Results 61 to 70 of about 44,813 (273)
BMC Biotechnology, 2021 Background Nattokinase is a fibrinolytic enzyme that has huge market value as a nutritional supplement for health promotion. In order to increase nattokinase yields, fermentation conditions, strains, cultivation media, and feeding strategies have been ...
Yan Guangbo +6 more
doaj +1 more source
Development Of High Cell Density Recombinant Pichia Pastoris Culture For Human Growth Hormone Production [PDF]
, 2008 Methylotrophic yeast, Pichia pastoris has been used as an excellent host to produce more than 400 recombinant heterologous proteins of academic and industrial interests.
Lee, Yoeu Chek
core
Microbial Cell Factories / Pichia pastoris Aft1 - a novel transcription factor, enhancing recombinant protein secretion [PDF]
, 2014 Background: The methylotrophic yeast Pichia pastoris is frequently used for the production of recombinant proteins. However, expression levels can vary depending on the target protein.
Buchetics, Markus +7 more
core +2 more sources
Biotechnology and Bioengineering, EarlyView.ABSTRACT Isobutanol is a fusel alcohol that can be produced microbially for use as a biofuel or upgraded into sustainable aviation fuel (SAF). A key enzyme in the isobutanol biosynthetic pathway is 2‐ketoacid decarboxylase (KDC), which irreversibly decarboxylates 2‐ketoisovalerate (KIV) to yield isobutyraldehyde.
Joshua J. Dietrich +6 more
wiley +1 more source
Synthetic Core Promoters for Pichia pastoris
ACS Synthetic Biology, 2013 Synthetic promoters are commonly used tools for circuit design or high level protein production. Promoter engineering efforts in yeasts, such as Saccharomyces cerevisiae and Pichia pastoris have mostly been focused on altering upstream regulatory sequences such as transcription factor binding sites.
Vogl, Thomas +4 more
openaire +2 more sources
An improved CRISPRi system in Pichia pastoris
Synthetic and Systems Biotechnology, 2023 CRISPR interference (CRISPRi) has been developed and widely used for gene repression in various hosts. Here we report an improved CRISPRi system in Pichia pastoris by fusing dCas9 with endogenous transcriptional repressor domains. The CRISPRi system shows strong repression of eGFP, with the highest efficiency of 85%.
Shujing Qiao +4 more
openaire +3 more sources
Electronic Journal of Biotechnology, 2016 Background: A thermostable lipase MAS1 from marine Streptomyces sp. strain was considered as a potential biocatalyst for industrial application, but its production level was relatively low.
Dongming Lan +3 more
doaj +1 more source
Cellobiohydrolase B of Aspergillus niger over-expressed in Pichia pastoris stimulates hydrolysis of oil palm empty fruit bunches [PDF]
, 2017 Background. Aspergillus niger, along with many other lignocellulolytic fungi, has been widely used as a commercial workhorse for cellulase production.
Bakar, F. D. A. +6 more
core +3 more sources
The power of many: when genetics met yeasts and high‐throughput
Biological Reviews, EarlyView.ABSTRACT In recent years, complex technological capabilities have evolved, driven by the need to solve complex and integrative biological questions through global analyses. New equipment allows the scaling up and automation of processes which previously were carried out on a very limited scale.
Víctor A. Tallada, Víctor Carranco
wiley +1 more source
Autolysis of Pichia pastoris induced by cold [PDF]
AMB Express, 2017 The production of recombinant biopharmaceutical proteins is a multi-billion dollar market. Protein recovery represents a major part of the production costs. Pichia pastoris is one of the microbial systems most used for the production of heterologous proteins.
Yaneth Bartolo-Aguilar +6 more
openaire +3 more sources