Results 61 to 70 of about 45,758 (267)

Energy Charge as an Indicator of Pexophagy in Pichia pastoris [PDF]

Frontiers in Microbiology, 2017
Pichia pastoris is a good model for pexophagy research owing to its diverse pexophagy modes (macropexophagy and micropexophagy) exhibited during carbon-source shift from methanol to other carbon sources. The critical condition that triggers activation of macropexophagy and micropexophagy is important for clarifying the P.
Taiyu Liu, Jianguo Zhang
openaire   +3 more sources

Scalable protein production by Komagataella phaffii enabled by ARS plasmids and carbon source-based selection

Microbial Cell Factories
Background Most recombinant Komagataella phaffii (Pichia pastoris) strains for protein production are generated by genomic integration of expression cassettes.
Florian Weiss, Guillermo Requena-Moreno, Carsten Pichler, Francisco Valero, Anton Glieder, Xavier Garcia-Ortega   +5 more
doaj   +1 more source

A Study and Application of Biocatalytic Synthesis of (S)-N-Boc-3-hydroxypiperidine

MATEC Web of Conferences, 2015
This paper first uses the environmental friendly whole cell of biocatalyst pichia pastoris SIT2014 to asymmetrically synthesized anti-tumor drug of chiral intermediate (S)-N-Boc-3-hydroxypiperidine.. Improve the final biocatalytic reduction yield to 85.4%
Chen Ying, Xu Yi, Chen Jianbo
doaj   +1 more source

Insect‐Microbe‐Based Laccase: Untapped Natural Resource for Industrial and Biotechnological Applications

Advanced Sustainable Systems, EarlyView.
The insect‐microbe interplay fulfills a key ecological role involving the degradation of ligninocellulose biomass. This is coordinated by the secretion of different kinds of proteins, of which ligninolytic enzymes, such as laccases (Lacs), are of prime importance.
Michael Dare Asemoloye, Adeyinka Michael Gbadebo, Tunde Sheriffdeen Bello, Olumayowa Mary Olowe, Akinlolu Olalekan Akanmu, Marta Elisabetta Eleonora Temporiti, Adeyinka Odebode, Segun Oladiran Babarinde, Tsigereda Weldemichael, Leonard Chitongo, Solveig Tosi, Mario Andrea Marchisio   +11 more
wiley   +1 more source

Pichia pastoris as a Host System for Transformations [PDF]

Molecular and Cellular Biology, 1985
We developed a methylotrophic yeast, Pichia pastoris, as a host for DNA transformations. The system is based on an auxotrophic mutant host of P. pastoris which is defective in histidinol dehydrogenase. As a selectable marker, we isolated and characterized the P. pastoris HIS4 gene. Plasmid vectors which contained either the P.
James M. Cregg, A Y Hessler, Kevin J. Barringer, Knut Madden   +3 more
openaire   +2 more sources

Effect of Multiple Gene Copy Number of Bacterial Lipase to Increase Lipase Production in Pichia pastoris

Hayati Journal of Biosciences
Bacterial lipase poses potential challenges when expressed in eukaryotic protein expression systems such as Pichia pastoris. This research aims to increase extracellular T1.2RQ lipase secretion (free lipase) with multiple gene copy number strategies in ...
Esti Puspitasari, Yepy Hardi Rustam, Andreas Adhi Satya, Antonius Suwanto, Aris Tri Wahyudi, Rika Indri Astuti   +5 more
doaj   +1 more source

Amyloid Forming Human Lysozyme Intermediates are Stabilized by Non‐Native Amide‐π Interactions

Advanced Science, EarlyView.
Mutational variants of human lysozyme cause fatal systemic amyloidosis by depositing kilograms of protein in the viscera of patients. Central to this process is a partially unfolded protein intermediate state that initiates aggregation into amyloid fibrils.
Minkoo Ahn, Julian O. Streit, Christopher A. Waudby, Tomasz Włodarski, Angelo Miguel Figueiredo, John Christodoulou, Janet R. Kumita   +6 more
wiley   +1 more source

Erratum to: Comparative genomics and transcriptomics of Pichia pastoris [PDF]

BMC Genomics, 2016
Erratum: In the original publication of this article [1], the accession numbers for the genomes and transcriptome listed are incorrect. The correct details of the NCBI accession numbers can be found below: Availability of data and materials: The genomic sequencing data and assembled and annotated genomes are deposited at NCBI under bioproject accession
Margaret Priest, Charles A. Whittaker, J. Christopher Love, J. Christopher Love, Sarah Young, Duanduan Ma, Kerry R. Love, Jie Wu, M. Catherine Bartlett, Jonathan Borowsky, Rachel Leeson, Kartik A. Shah   +11 more
openaire   +5 more sources

Decreased expression of LRA4, a key gene involved in rhamnose metabolism, caused up-regulated expression of the genes in this pathway and autophagy in Pichia pastoris

AMB Express, 2020
In a previous study, we developed Pichia pastoris GS115m, an engineered strain with decreased expression of one key gene, LRA4, in rhamnose metabolism. P.
Jian Jiao, Shuai Wang, Hui Tian, Xinxin Xu, Yuhong Zhang, Bo Liu, Wei Zhang   +6 more
doaj   +1 more source

Heterologous expression of nattokinase from B. subtilis natto using Pichia pastoris GS115 and assessment of its thrombolytic activity

BMC Biotechnology, 2021
Background Nattokinase is a fibrinolytic enzyme that has huge market value as a nutritional supplement for health promotion. In order to increase nattokinase yields, fermentation conditions, strains, cultivation media, and feeding strategies have been ...
Yan Guangbo, Shu Min, Shen Wei, Ma Lixin, Zhai Chao, Wang Yaping, Huang Zunxi   +6 more
doaj   +1 more source