Results 71 to 80 of about 44,813 (273)

Effect of cell retention techniques in Komagataella phaffii lab‐scale continuous processes

Biotechnology Progress, EarlyView.
Abstract Perfusion technologies play a growing role in the implementation of continuous processes for biotherapeutics production in mammalian‐based manufacturing. However, their application to alternative production hosts is limited. Cell retention systems are of key importance for the efficiency of perfusion bioreactors.
Marina Y. Linova, Satish K. Kodiripaka, Edite Martins, Sobhana A. Sripada, Stefano Menegatti, John M. Woodley   +5 more
wiley   +1 more source

Development of quantitative metabolomics for Pichia pastoris [PDF]

Metabolomics, 2011
Accurate, reliable and reproducible measurement of intracellular metabolite levels has become important for metabolic studies of microbial cell factories. A first critical step for metabolomic studies is the establishment of an adequate quenching and washing protocol, which ensures effective arrest of all metabolic activity and removal of extracellular
Carnicer,, M. (author), Canelas, A.B. (author), Ten Pierick, A. (author), Zeng, Z. (author), Van Dam, J. (author), Albiol, J. (author), Ferrer, P. (author), Heijnen, J.J. (author), Van Gulik, W. (author)   +8 more
openaire   +4 more sources

Study on Multi-Model Soft Sensor Modeling Method and Its Model Optimization for the Fermentation Process of Pichia pastoris

Sensors, 2021
The problems that the key biomass variables in Pichia pastoris fermentation process are difficult measure in real time; this paper mainly proposes a multi-model soft sensor modeling method based on the piecewise affine (PWA) modeling method, which is ...
Bo Wang, Xingyu Wang, Mengyi He, Xianglin Zhu   +3 more
doaj   +1 more source

Assessment of Metabolites in Noni Fruit Slurry Fermented With Four Non‐Saccharomyces Yeasts

Food Frontiers, EarlyView.
Dried noni fruit slurry (DNFS) was fermented for 14 days with selected non‐Saccharomyces yeasts. GCMS, HPLC and amino acid analysis showed that Pichia kluyveri Frootzen and Williopsis saturnus NCYC22 grew well, reduced off‐flavor fatty acids, generated fruity aroma compounds and preserved total phenolic content. ABSTRACT Noni products often suffer from
Zhuoran Zhang, Shao Quan Liu, Yuyun Lu
wiley   +1 more source

Production of enterocin P by a bioengineered Pichia pastoris to control of pathogen bacteria [PDF]

Journal of Epigenetics
Entrocin p has been characterized in class IIa bacteriocins and showed a broad spectrum of antimicrobial activity. In this study, the nucleotide sequence of enterocin P transformed and expressed in Pichia pastoris genomically, and antimicrobial activity ...
Abbas Tanhaeian, Reza Majidzadeh Heravi, bahareh pakbaten   +2 more
doaj   +1 more source

Novel homologous lactate transporter improves l‑lactic acid production from glycerol in recombinant strains of Pichia pastoris [PDF]

, 2016
Background: Crude glycerol is the main byproduct of the biodiesel industry. Although it can have different applications, its purification is costly. Therefore, in this study a biotechnological route has been proposed for further utilization of crude ...
Carvalho, Lucas Silva, Castro, Virgilio H. de, Goldman, Gustavo Henrique, Lima, Pollyne Borborema Almeida de, Magalhães, Beatriz Simas, Melo, Nadiele Tamires Moreira, Menino, Gisele Soares, Mulder, Kelly Cristina Leite, Mulinari, Eduardo, Parachi, Nádia Skorupa, Reis, Thaila Fernanda dos   +10 more
core   +1 more source

Chemical Characterization of Postindustrial Recycled Polyethylene Terephthalate Obtained From Multilayer PET/PE Sheets: A Baseline Study on Safety Aspects

Packaging Technology and Science, EarlyView.
PET trays pose recycling challenges due to multilayer structures with polyethylene (PE) tie‐layers and barrier materials. In this study, a delamination process using a hot alkaline solution was applied to postindustrial multilayer PET/PE thermoforming waste to recover PET flakes and produce sheets.
Isabela F. A. Daras, Srishti Singh, Tiago Vieira, Joel Pereira, Patrícia Guerreiro, Clara Sousa, Fátima Poças   +6 more
wiley   +1 more source

Improved production of a recombinant lipase expressed in and its application for conversion of microalgae oil to biodiesel [PDF]

, 2013
Part 2: Trust and PrivacyInternational audienceTrust and privacy have been widely studied as key issues and success factors for e-commerce. The advent of m-commerce calls for revisiting these concepts and re-examining their antecedents in the mobile ...
Jinjin Huang, Ji Xia, Zhen Yang, Feifei Guan, Di Cui, Guohua Guan, Wei Jiang, Ying Li   +7 more
core   +2 more sources

The return of metabolism: biochemistry and physiology of glycolysis

Biological Reviews, EarlyView.
ABSTRACT Glycolysis is a fundamental metabolic pathway central to the bioenergetics and physiology of virtually all living organisms. In this comprehensive review, we explore the intricate biochemical principles and evolutionary origins of glycolytic pathways, from the classical Embden–Meyerhof–Parnas (EMP) pathway in humans to various prokaryotic and ...
Nana‐Maria Grüning, Federica Agostini, Camila Caldana, Johannes Hartl, Matthias Heinemann, Markus A. Keller, Jan Lukas Krüsemann, Costanza Lamperti, Carole L. Linster, Steffen N. Lindner, Julia Muenzner, Jens Nielsen, Zoran Nikoloski, Bettina Siebers, Jacky L. Snoep, Hezi Tenenboim, Bas Teusink, Spencer J. Williams, Mirjam M. C. Wamelink, Markus Ralser   +19 more
wiley   +1 more source

Optimization of expression conditions for a novel NZ2114-derived antimicrobial peptide-MP1102 under the control of the GAP promoter in Pichia pastoris X-33 [PDF]

, 2015
BACKGROUND: The infections caused by antibiotic multidrug-resistant bacteria seriously threaten human health. To prevent and cure the infections caused by multidrug-resistant bacteria, new antimicrobial agents are required.
Da Teng, Jian Jiao, Jianhua Wang, Ruoyu Mao, Xintao Cao, Xiumin Wang, Yong Zhang   +6 more
core   +1 more source