Results 81 to 90 of about 45,758 (267)

Establishing a Eukaryotic Pichia pastoris Cell-Free Protein Synthesis System

Frontiers in Bioengineering and Biotechnology, 2020
In recent years, cell-free protein synthesis (CFPS) systems have been used to synthesize proteins, prototype genetic elements, manufacture chemicals, and diagnose diseases.
Lingkai Zhang, Wanqiu Liu, Jian Li
semanticscholar   +1 more source

Actin Filament Pointed Ends: Assays for Regulation of Assembly and Disassembly by Tropomodulin and Tropomyosin

Cytoskeleton, EarlyView.
ABSTRACT Actin filaments are dynamic polymers whose length depends on regulated monomer association and dissociation at their ends. Actin barbed‐end dynamics are relatively better understood, primarily due to the approximately tenfold faster subunit on/off rates at barbed versus pointed ends.
Sawako Yamashiro, Shashank Shekhar, Stefanie M. Novak, Sudipta Biswas, Carol C. Gregorio, Velia M. Fowler   +5 more
wiley   +1 more source

Bio‐Based Solvents Extraction and Enzyme‐assisted Recovery of Furan Fatty Acid From Ammonia‐Stabilized Hevea Latex

European Journal of Lipid Science and Technology, EarlyView.
The latex of Hevea brasiliensis is rich in furan fatty acid (FuFA) esterified in both neutral and polar lipids. Bio‐based solvents to extract FuFA from ammonia‐stabilized latex was tested. Ethyl acetate worked best, especially with low‐ammonia content. Isopropanol was a good alternative. Lipases could also help to efficiently release FuFA. The findings
Chaisiri Kitpaosong, Siriluck Liengprayoon, Tucksin Lerksamran, Kittipong Rattanaporn, Bruno Barea, Achille Marchand, Frédéric Carrière, Hélène Launay, Thongchai Khaeduang, Pisamai Chantuma, Laurent Vaysse, Erwann Durand   +11 more
wiley   +1 more source

Correction to: Autolysis of Pichia pastoris induced by cold [PDF]

AMB Express, 2018
The original version of this article (Bartolo-Aguilar et al. 2017) was written and published including the first construction strategy of pLGC09, but not the final one. This error was pointed out by a reader and an analysis of sequences of parts of the plasmid corroborated this.
Yaneth Bartolo-Aguilar, Luc Dendooven, Cipriano Chávez-Cabrera, Luis B. Flores-Cotera, María E. Hidalgo-Lara, Lourdes Villa-Tanaca, Rodolfo Marsch   +6 more
openaire   +4 more sources

Comprehensive comparison of Yarrowia lipolytica and Pichia pastoris for production of Candida antarctica lipase B

Scientific Reports, 2020
The large-scale production of recombinant proteins (rProt) is becoming increasingly economically important. Among the different hosts used for rProt production, yeasts are gaining popularity.
C. Theron, M. Vandermies, Samuel Telek, Sébastien Steels, P. Fickers   +4 more
semanticscholar   +1 more source

Biotechnological Potential of the Fusarium fujikuroi Complex From Amazonian Oenocarpus bacaba: Lipase Production and Characterization

Journal of Basic Microbiology, EarlyView.
ABSTRACT This study investigates the diversity of filamentous fungi in the Amazon biome, particularly the genus Fusarium, known for producing biotechnologically valuable metabolites. The research aimed to isolate and identify fungi from Oenocarpus bacaba Mart.
Lucely Nogueira dos Santos, Rafael Firmani Perna, Ana Carolina Vieira, Kalebe Ferreira Furtado, Alex Fernando de Almeida, Cristiane Angélica Ottoni, Leandro Mantovani de Castro, Felipe Mello, Yoannis Domínguez, Nelson Rosa Ferreira   +9 more
wiley   +1 more source

Genetic tools for metabolic engineering of Pichia pastoris

Engineering Microbiology, 2023
The methylotrophic yeast Pichia pastoris (also known as Komagataella phaffii) is widely used as a yeast cell factory for producing heterologous proteins. Recently, it has gained attention for its potential in producing chemicals from inexpensive feedstocks, which requires efficient genetic engineering platforms.
Xiaoyan Wu, Peng Cai, Lun Yao, Yongjin J Zhou   +3 more
openaire   +3 more sources

Uncovering Sequence and Structural Characteristics of Fungal Expansin‐Related Proteins With Potential to Drive Substrate Targeting

Proteins: Structure, Function, and Bioinformatics, EarlyView.
ABSTRACT Expansins loosen plant cell wall networks through disrupting non‐covalent bonds between cellulose microfibrils and matrix polysaccharides. Whereas expansins were first discovered in plants, expansin‐related proteins have since been identified in bacteria and fungi.
Anna Pohto, Taru Koitto, Deepika Dahiya, Alessandra Castro, Elizaveta Sidorova, Martina Huusela, Scott E. Baker, Adrian Tsang, Emma Master   +8 more
wiley   +1 more source

A roadmap to establish a comprehensive platform for sustainable manufacturing of natural products in yeast

Nature Communications, 2023
Yeasts, Saccharomyces cerevisiae and Pichia pastoris, are promising chassis for the production of nature products (NPs). Here, the author discusses establishing a comprehensive platform for sustainable production of NPs via system-associated optimization
Gita Naseri
doaj   +1 more source

Development of a New High-Cell Density Fermentation Strategy for Enhanced Production of a Fungus β-Glucosidase in Pichia pastoris

Frontiers in Microbiology, 2020
Traditional diosgenin manufacturing process has led to serious environmental contamination and wastewater. Clean processes are needed that can alternate the diosgenin production. The β-glucosidase FBG1, cloned from Fusarium sp.
Wancang Liu, Haibo Xiang, Tao Zhang, Xu Pang, Jing Su, Hongyu Liu, B. Ma, Liyan Yu   +7 more
semanticscholar   +1 more source