Results 81 to 90 of about 44,813 (273)

Modifying inhibitor specificity for homologous enzymes by machine learning

The FEBS Journal, EarlyView.
We present a machine learning method for the design of selective enzyme inhibitors, reducing experimental effort thus accelerating the development of targeted therapeutics. Trained on existing data for three related matrix metalloproteinases (MMPs), our method successfully designed a novel inhibitor variant with enhanced selectivity for one MMP over ...
Dor S. Gozlan, Reut Meiri, Gili Shapira, Matt Coban, Evette S. Radisky, Yaron Orenstein, Niv Papo   +6 more
wiley   +1 more source

PpAtg9 Trafficking During Micropexophagy in Pichia pastoris [PDF]

Autophagy, 2006
PpAtg9 is essential for the selective degradation of peroxisomes (e.g., pexophagy) in Pichia pastoris. This integral membrane protein is synthesized in the endoplasmic reticulum (ER) and transported to a unique peripheral compartment (Atg9-PC). A putative ER exit motif has been identified and when deleted results in the accumulation of PpAtg9 within ...
Laura A, Schroder, William A, Dunn
openaire   +2 more sources

Citrobacter amalonaticus phytase on the cell surface of Pichia pastoris exhibits high pH stability as a promising potential feed supplement. [PDF]

PLoS ONE, 2014
Phytase expressed and anchored on the cell surface of Pichia pastoris avoids the expensive and time-consuming steps of protein purification and separation. Furthermore, yeast cells with anchored phytase can be used as a whole-cell biocatalyst.
Cheng Li, Ying Lin, Yuanyuan Huang, Xiaoxiao Liu, Shuli Liang   +4 more
doaj   +1 more source

Quantitative metabolomics analysis of amino acid metabolism in recombinant Pichia pastoris under different oxygen availability conditions [PDF]

, 2012
Background: Environmental and intrinsic stress factors can result in the global alteration of yeast physiology, as evidenced by several transcriptional studies.
Albiol i Sala, Joan, Carnicer Heras, Marc, Ferrer, Pau, Heijnen, Joseph J., Ten Pierick, Angela, van Dam, Jan, van Gulik, Walter   +6 more
core   +4 more sources

A Fungal Cysteine‐Rich Protein SsSCP Activates Immune Responses and Confers Broad‐Spectrum Disease Resistance in Tomato

Plant Biotechnology Journal, EarlyView.
ABSTRACT Plant immune systems recognise pathogen‐derived molecular patterns to activate defence responses, yet the mechanisms underlying fungal pattern recognition in crops remain largely unclear. Here, we characterise the cysteine‐rich secretory protein SsSCP from the broad‐host‐range fungal pathogen Sclerotinia sclerotiorum as a potent elicitor of ...
Liyuan Zhang, Yuan Wang, Yuhang Liu, Jinwen Yang, Kaihao Zhang, Chengzhang Du, Jiajing Xiao, Ling Qing, Xinhua Sun   +8 more
wiley   +1 more source

Cloning of Synthetic Lipase Gene from Rhizomucor miehei with Original Signal Peptide in Pichia pastoris

Microbiology Indonesia, 2017
Lipases (EC 3.1.1.3) are classified as hydrolases that hydrolyze lipids. These enzymes have potential application in biotechnology and industrial process. In previous study we have cloned the synthetic Rhizomucor miehei lipase gene using the vector pUC57
doaj   +1 more source

Glucuronoyl Esterase of Pathogenic Phanerochaete carnosa Induces Immune Responses in Aspen Independently of Its Enzymatic Activity

Plant Biotechnology Journal, EarlyView.
ABSTRACT Microbial enzymes expressed in plants add new functionalities but occasionally trigger undesirable immune responses. Phanerochaete carnosa glucuronoyl esterase (PcGCE) hydrolyses the bond between lignin and 4‐O‐methyl‐α‐D‐glucuronic acid substituent of glucuronoxylan.
Evgeniy N. Donev, Marta Derba‐Maceluch, Xiao‐Kun Liu, Henri Colyn Bwanika, Izabela Dobrowolska, Mohit Thapa, Joanna Leśniewska, Jan Šimura, Alex Yi‐Lin Tsai, Konrad S. Krajewski, Dan Boström, Leszek A. Kleczkowski, Maria E. Eriksson, Karin Ljung, Emma R. Master, Ewa J. Mellerowicz   +15 more
wiley   +1 more source

A roadmap to establish a comprehensive platform for sustainable manufacturing of natural products in yeast

Nature Communications, 2023
Yeasts, Saccharomyces cerevisiae and Pichia pastoris, are promising chassis for the production of nature products (NPs). Here, the author discusses establishing a comprehensive platform for sustainable production of NPs via system-associated optimization
Gita Naseri
doaj   +1 more source

Uncovering Convergent Pattern Recognition Receptors Recognising Phytophthora Across Plant Lineages

Plant Biotechnology Journal, EarlyView.
ABSTRACT Pattern recognition receptors (PRRs) are pivotal for plant immunity, yet their discovery in crops is hindered by lineage‐specific divergence. We demonstrate that microbe‐associated molecular patterns (MAMPs) often activate immunity through phylogenetically unrelated, convergently evolved PRRs across plant lineages.
Yong Pei, Yaning Zhao, Hui Wang, Yining Guo, Xinyi Gu, Jingkun Lv, Zhenjie Guo, Yanjun Chen, Yingkai Ren, Yanrong Ren, Jianyu Yan, Yuke Wang, Peiyun Ji, Danyu Shen, Zhiyuan Yin, Daolong Dou   +15 more
wiley   +1 more source

Engineering of 10‐Deacetylbaccatin III‐10‐β‐O‐Acetyltransferase From Taxus Species for Efficient Acetylating Non‐Natural Substrates Into Taxol in Nicotiana benthamiana

Plant Biotechnology Journal, EarlyView.
ABSTRACT 7‐β‐xylosyl‐10‐deacetyltaxol (XDT) is much more abundant than the anticancer drug Taxol in Taxus species and is usually regarded as the by‐product of Taxol. It could be enzymatically transformed into 10‐deacetyltaxol (DT), and the latter could be further converted into Taxol.
Tian‐Jiao Chen, Jing‐Jing Chen, Xiao‐Yan Sun, Ting Gong, Jin‐Ling Yang, Ping Zhu   +5 more
wiley   +1 more source