Cell-free study of F plasmid partition provides evidence for cargo transport by a diffusion-ratchet mechanism. [PDF]
Proc Natl Acad Sci U S A, 2013 Vecchiarelli AG, Hwang LC, Mizuuchi K.
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Centromere binding specificity in assembly of the F plasmid partition complex. [PDF]
Nucleic Acids Res, 2011 The segregation of plasmid F of Escherichia coli is highly reliable. The Sop partition locus, responsible for this stable maintenance, is composed of two genes, sopA and sopB and a centromere, sopC, consisting of 12 direct repeats of 43 bp.
Pillet F +4 more
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Order and disorder in the domain organization of the plasmid partition protein KorB. [PDF]
J Biol Chem, 2010 The plasmid partition protein KorB has a dual role: it is essential for the correct segregation of the low copy number broad host range RK2 plasmid while also being an important regulator of transcription. KorB belongs to the ParB family of proteins, and
Rajasekar K +9 more
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Plasmid segregation without partition [PDF]
Mobile Genetic Elements, 2011 Low-copy number plasmids need a segregation mechanism to assort one half of the plasmid copies to each daughter cell during cell division. This can be achieved directly by partitioning plasmid copies through a mechanism reminiscent of eukaryotic mitosis.
Guynet, Catherine, Cruz, Fernando de la
openaire +3 more sources
ATP-regulated interactions between P1 ParA, ParB and non-specific DNA that are stabilized by the plasmid partition site, parS. [PDF]
Nucleic Acids Res, 2012 Localization of the P1 plasmid requires two proteins, ParA and ParB, which act on the plasmid partition site, parS. ParB is a site-specific DNA-binding protein and ParA is a Walker-type ATPase with non-specific DNA-binding activity.
Havey JC, Vecchiarelli AG, Funnell BE.
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Mathematical Models of Plasmid Population Dynamics
Frontiers in Microbiology, 2021 With plasmid-mediated antibiotic resistance thriving and threatening to become a serious public health problem, it is paramount to increase our understanding of the forces that enable the spread and maintenance of drug resistance genes encoded in mobile ...
José Carlos Ramón Hernández-Beltrán +3 more
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Defining the role of ATP hydrolysis in mitotic segregation of bacterial plasmids. [PDF]
PLoS Genetics, 2013 Hydrolysis of ATP by partition ATPases, although considered a key step in the segregation mechanism that assures stable inheritance of plasmids, is intrinsically very weak.
Yoan Ah-Seng +3 more
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ATP control of dynamic P1 ParA-DNA interactions: a key role for the nucleoid in plasmid partition. [PDF]
Mol Microbiol, 2010 P1 ParA is a member of the Walker‐type family of partition ATPases involved in the segregation of plasmids and bacterial chromosomes. ATPases of this class interact with DNA non‐specifically in vitro and colocalize with the bacterial nucleoid to generate
Vecchiarelli AG +8 more
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Frontiers in Molecular Biosciences, 2020 The molecular events that underpin genome segregation during bacterial cytokinesis have not been fully described. The tripartite segrosome complex that is encoded by the multiresistance plasmid TP228 in Escherichia coli is a tractable model to decipher ...
Marisa Caccamo +5 more
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Plasmid stability analysis based on a new theoretical model employing stochastic simulations. [PDF]
PLoS ONE, 2017 Here, we present a simple theoretical model to study plasmid stability, based on one input parameter which is the copy number of plasmids present in a host cell.
Olesia Werbowy +2 more
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