Results 81 to 90 of about 980,977 (401)

Microglial dynamics and ferroptosis induction in human iPSC‐derived neuron–astrocyte–microglia tri‐cultures

FEBS Open Bio, EarlyView.
A tri‐culture of iPSC‐derived neurons, astrocytes, and microglia treated with ferroptosis inducers as an Induced ferroptosis model was characterized by scRNA‐seq, cell survival, and cytokine release assays. This analysis revealed diverse microglial transcriptomic changes, indicating that the system captures key aspects of the complex cellular ...
Hongmei Lisa Li, Hiroko Ohmiya, Sou Sakamoto, Masato Yugami, Akiko Oki, Makoto Furusawa, Yan Ling   +6 more
wiley   +1 more source

Gain of 20q11.21 in human pluripotent stem cells impairs TGF-β-dependent neuroectodermal commitment [PDF]

, 2019
Gain of 20q11.21 is one of the most common recurrent genomic aberrations in human pluripotent stem cells. Although it is known that overexpression of the antiapoptotic gene Bcl-xL confers a survival advantage to the abnormal cells, their differentiation ...
De Deckersberg, E. Couvreu, De Kock, J., Dziedzicka, D., Franceschini, L., Geens, M., Keller, A., Markouli, C., Nguyen, H. T., Regin, M., Sermon, K., Spits, C., Tilleman, Laurentijn, Van Nieuwerburgh, Filip, Zambelli, F.   +13 more
core   +1 more source

Gametogenesis from Pluripotent Stem Cells [PDF]

Cell Stem Cell, 2016
The germ cell lineage originates early in development and undergoes a series of complex developmental processes that culminate in the generation of fully matured gametes, the spermatozoa and the oocytes. Remarkably, researchers have been recapitulating these developmental pathways using mouse and human pluripotent stem cells (PSCs).
Saitou, Mitinori, Miyauchi, Hidetaka
openaire   +2 more sources

Hair-bearing human skin generated entirely from pluripotent stem cells

bioRxiv, 2019
The skin is a multilayered organ, equipped with appendages (that is, follicles and glands), that is critical for regulating body temperature and the retention of bodily fluids, guarding against external stresses and mediating the sensation of touch and ...
Jiyoon Lee, Cyrus C. Rabbani, Hongyu Gao, Matthew R. Steinhart, Benjamin M. Woodruff, Zachary E. Pflum, Alexander B. Kim, S. Heller, Yunlong Liu, Taha Z. Shipchandler, Karl R. Koehler   +10 more
semanticscholar   +1 more source

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

FEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li, Xi Xiao, Lingbin Qi, Chanyi Li, Sirui Song, Jiaying Qin, Zhigang Xue, Jinfeng Xue, Huaifang Li   +8 more
wiley   +1 more source

Multiple feedback loops through cytokinin signaling control stem cell number within the Arabidopsis shoot meristem [PDF]

, 2009
A central unanswered question in stem cell biology, both in plants and in animals, is how the spatial organization of stem cell niches are maintained as cells move through them.
Anders, Birnbaum, Brand, C. Ohno, Clark, Clark, Clark, Clark, E. M. Meyerowitz, Fletcher, Fletcher, Gallois, Gallois, Geier, Gordon, Haecker, Jasinski, Jonsson, Kayes, Kurakawa, Laux, Leibfried, Long, Mahonen, Mayer, Muller, Muller, S. P. Gordon, Sablowski, Schoof, Skoog, Tucker, V. S. Chickarmane, Yanai   +33 more
core   +2 more sources

Transcriptionally Active HERV-H Retrotransposons Demarcate Topologically Associating Domains in Human Pluripotent Stem Cells

Nature Genetics, 2019
Chromatin architecture has been implicated in cell type-specific gene regulatory programs, yet how chromatin remodels during development remains to be fully elucidated.
Yanxiao Zhang, Ting Li, Sebastian Preissl, Maria Luisa Amaral, Jonathan D. Grinstein, Elie N. Farah, Eugin Destici, Yunjiang Qiu, Rong Hu, Ah Young Lee, Sora Chee, Kaiyue Ma, Z. Ye, Quan-sheng Zhu, Hui Huang, Rongxin Fang, Leqian Yu, J. I. Izpisúa Belmonte, Jun Wu, S. Evans, N. Chi, B. Ren   +21 more
semanticscholar   +1 more source

Developmental, Neuroanatomical and Cellular Expression of Genes Causing Dystonia

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Dystonia is one of the most common movement disorders, with variants in multiple genes identified as causative. However, an understanding of which developmental stages, brain regions, and cell types are most relevant is crucial for developing relevant disease models and therapeutics.
Darren Cameron, Nicholas E. Clifton, Daniel Cabezas de la Fuente, Peter Holmans, Nicholas J. Bray, Kathryn J. Peall   +5 more
wiley   +1 more source

Glucokinase (GCK) in diabetes: from molecular mechanisms to disease pathogenesis

Cellular & Molecular Biology Letters
Glucokinase (GCK), a key enzyme in glucose metabolism, plays a central role in glucose sensing and insulin secretion in pancreatic β-cells, as well as glycogen synthesis in the liver.
Yasmin Abu Aqel, Aldana Alnesf, Idil I. Aigha, Zeyaul Islam, Prasanna R. Kolatkar, Adrian Teo, Essam M. Abdelalim   +6 more
doaj   +1 more source

3D (Bio) Printing Combined Fiber Fabrication Methods for Tissue Engineering Applications: Possibilities and Limitations

Advanced Functional Materials, EarlyView.
Biofabrication aims at providing innovative technologies and tools for the fabrication of tissue‐like constructs for tissue engineering and regenerative medicine applications. By integrating multiple biofabrication technologies, such as 3D (bio) printing with fiber fabrication methods, it would be more realistic to reconstruct native tissue's ...
Waseem Kitana, Indra Apsite, Leonid Ionov   +2 more
wiley   +1 more source