Results 241 to 250 of about 39,468 (302)

Endogenous Protein Tagging in Postmitotic Neurons via Lentiviral and Adeno‐associated Virus Delivery

Current Protocols, Volume 6, Issue 10, October 2026.
Abstract This protocol describes a method for endogenous protein tagging in postmitotic primary neurons using homology‐independent targeted integration (HITI), a CRISPR/Cas9‐based approach that exploits non‐homologous end joining (NHEJ). Unlike homology‐directed repair (HDR), HITI does not require cell division, making it uniquely suited for ...
Lorena Benedetti, Hyun Ah Yi, Lihua Wang, Jennifer Lippincott‐Schwartz   +3 more
wiley   +1 more source

Engineered dual-responsive mucoadhesive hydrogel co-delivering fullerenol against radiation-induced vaginal injury and microbiota dysbiosis. [PDF]

Mater Today Bio
Zhao M, Wang C, Huang Y, Wang L, Xiao J, Gu Z.   +5 more
europepmc   +1 more source

Müller Glia–Vasculature Interactions in the Developing Retina

Glia, Volume 74, Issue 10, October 2026.
Retinal vascular layers form normally despite loss of cholinergic retinal waves. Müller glial processes contact endothelial tip cells early and form Aquaporin‐4‐enriched endfeet. Glial endfoot calcium signals are compartmentalized and occur largely independently of neural activity. ABSTRACT Coordinated signaling among neurons, glia, and the vasculature
Samira Monshietehadi, Angel J. Garcia, Benjamin E. Smith, Matthew Tarchick, Kiara Abhayaratne, Aileen Conley, Marla B. Feller   +6 more
wiley   +1 more source

Calcium Imaging in H4IIE Liver Cells and Primary Rodent Hepatocytes: A Cost-Effective Protocol for Use With Fura-2 AM Ca²⁺ Indicator. [PDF]

Bio Protoc
Fang L, Habib AGK, Harris MD, Begley AJ, Ali ES.   +4 more
europepmc   +1 more source

3D printed chip as platform to vascularize hiPSCs-derived kidney organoids. [PDF]

Biomed Microdevices
Addario G, Formica C, Moroni L, Mota C.
europepmc   +1 more source

Reversible Microfluidic Platform for Spheroid Culturing, Downstream Characterization, and Dynamic Anticancer Susceptibility Testing. [PDF]

ACS Meas Sci Au
Sousa Ribeiro IR, da Silva PHN, Olsen BG, da Silva Vilarins K, Amador Souza EM, de Araujo Andreotti IA, Souza Barbosa LR, Carrilho E, Gobbi AL, Teodoro Martinez DS, Lima RS.   +10 more
europepmc   +1 more source

Injectable Thermoresponsive Dual Nanocarrier Hydrogel for Local Tacrolimus Delivery with a Two-Phase Release Profile. [PDF]

Pharmaceutics
Srishti SA, Pinky PP, Shill DK, Surti V, Janjic JM.   +4 more
europepmc   +1 more source

Angiogenin alleviates lipotoxicity-induced impairment of skin wound healing. [PDF]

Cell Mol Biol Lett
Fu M, Wang Y, Wu C, Deng X, Zheng Z, Yu Y, Yu Y, Ma L, Feng J, Zhang C.   +9 more
europepmc   +1 more source