RNase E biomolecular condensates stimulate PNPase activity [PDF]
Scientific Reports, 2023 Bacterial Ribonucleoprotein bodies (BR-bodies) play an essential role in organizing RNA degradation via phase separation in the cytoplasm of bacteria. BR-bodies mediate multi-step mRNA decay through the concerted activity of the endoribonuclease RNase E ...
Michael J. Collins +4 more
doaj +7 more sources
Single-exonuclease nanocircuits reveal the RNA degradation dynamics of PNPase and demonstrate potential for RNA sequencing [PDF]
Nature Communications, 2023 Observing the natural process of RNA degradation in real-time is a significant challenge. Here, the authors develop and use single-exonuclease nanocircuits to reveal the single-base degradation behaviour of PNPase, and demonstrate proof-of-principle RNA ...
, Lidong Li, Xuefeng Guo
exaly +6 more sources
PNPase knockout results in mtDNA loss and an altered metabolic gene expression program
PLoS ONE, 2018 Polynucleotide phosphorylase (PNPase) is an essential mitochondria-localized exoribonuclease implicated in multiple biological processes and human disorders.
Fasih M Ahsan, Dian Huang, Tara TeSlaa
exaly +9 more sources
Human PNPase causes RNA stabilization and accumulation of R-loops in the Escherichia coli model system [PDF]
Scientific Reports, 2023 Polyribonucleotide phosphorylase (PNPase) is a phosphorolytic RNA exonuclease highly conserved throughout evolution. In Escherichia coli, PNPase controls complex phenotypic traits like biofilm formation and growth at low temperature.
Federica A. Falchi +7 more
doaj +6 more sources
A PNPase dependent CRISPR System in Listeria. [PDF]
PLoS Genetics, 2014 The human bacterial pathogen Listeria monocytogenes is emerging as a model organism to study RNA-mediated regulation in pathogenic bacteria. A class of non-coding RNAs called CRISPRs (clustered regularly interspaced short palindromic repeats) has been ...
Nina Sesto +9 more
doaj +9 more sources
Microbiology Spectrum, 2022 8-Oxo-7,8-dihydroguanine (8-oxoG) is a major RNA modification caused by oxidative stresses and has been implicated in carcinogenesis, neurodegeneration, and aging.
Runhua Han +3 more
doaj +5 more sources
Impact of PNPase on the transcriptome of Rhodobacter sphaeroides and its cooperation with RNase III and RNase E [PDF]
BMC Genomics, 2021 Background The polynucleotide phosphorylase (PNPase) is conserved among both Gram-positive and Gram-negative bacteria. As a core part of the Escherichia coli degradosome, PNPase is involved in maintaining proper RNA levels within the bacterial cell.
Daniel-Timon Spanka +2 more
doaj +4 more sources
PNPase is involved in the coordination of mRNA degradation and expression in stationary phase cells of Escherichia coli [PDF]
BMC Genomics, 2018 Background Exoribonucleases are crucial for RNA degradation in Escherichia coli but the roles of RNase R and PNPase and their potential overlap in stationary phase are not well characterized.
Clémentine Dressaire +5 more
doaj +3 more sources
Controlling the mitochondrial antisense – role of the SUV3-PNPase complex and its co-factor GRSF1 in mitochondrial RNA surveillance [PDF]
Molecular & Cellular Oncology, 2018 Transcription of the human mitochondrial genome produces a vast amount of non-coding antisense RNAs. These RNA species can form G-quadraplexes (G4), which affect their decay. We found that the mitochondrial degradosome, a complex of RNA helicase SUPV3L1 (
Zbigniew Pietras +8 more
doaj +3 more sources
Polynucleotide phosphorylase has an impact on cell biology of Campylobacter jejuni [PDF]
Frontiers in Cellular and Infection Microbiology, 2012 Polynucleotide phosphorylase, encoded by the pnp gene, is known to degrade mRNA, mediating post-transcriptional regulation and may affect cellular functions. The role of PNPase is pleiotropic.
Nabila eHaddad +12 more
doaj +2 more sources