Results 11 to 20 of about 4,629 (230)

Structural insights into human PNPase in health and disease. [PDF]

Nucleic Acids Res
Human polynucleotide phosphorylase (hPNPase) is a 3′-to-5′ exoribonuclease located in mitochondria, where it plays crucial roles in RNA degradation and RNA import.
Li YC, Wang CH, Patra M, Chen YP, Yang WZ, Yuan HS.   +5 more
europepmc   +5 more sources

Selective 8-oxo-rG stalling occurs in the catalytic core of polynucleotide phosphorylase (PNPase) during degradation. [PDF]

Proc Natl Acad Sci U S A
Significance Polynucleotide phosphorylase (PNPase) is a widely conserved exoribonuclease that degrades single-stranded RNA transcripts in the 3′ to 5′ direction, forming single-nucleotide diphosphates and a short oligonucleotide product.
Miller LG, Kim W, Schowe S, Taylor K, Han R, Jain V, Park R, Sherman M, Fang J, Ramirez H, Ellington A, Tamamis P, Resendiz MJE, Zhang YJ, Contreras L.   +14 more
europepmc   +3 more sources

Single-exonuclease nanocircuits reveal the RNA degradation dynamics of PNPase and demonstrate potential for RNA sequencing

Nature Communications, 2023
Observing the natural process of RNA degradation in real-time is a significant challenge. Here, the authors develop and use single-exonuclease nanocircuits to reveal the single-base degradation behaviour of PNPase, and demonstrate proof-of-principle RNA ...
Zhiheng Yang, Wenzhe Liu, Lihua Zhao, Dongbao Yin, Jianfei Feng, Lidong Li, Xuefeng Guo   +6 more
doaj   +4 more sources

Controlling the mitochondrial antisense – role of the SUV3-PNPase complex and its co-factor GRSF1 in mitochondrial RNA surveillance [PDF]

Molecular & Cellular Oncology, 2018
Transcription of the human mitochondrial genome produces a vast amount of non-coding antisense RNAs. These RNA species can form G-quadraplexes (G4), which affect their decay. We found that the mitochondrial degradosome, a complex of RNA helicase SUPV3L1 (
Zbigniew Pietras, Magdalena A. Wojcik, Lukasz S. Borowski, Maciej Szewczyk, Tomasz M. Kulinski, Dominik Cysewski, Piotr P. Stepien, Andrzej Dziembowski, Roman J. Szczesny   +8 more
doaj   +3 more sources

Role of the PNPase enzyme in the transport of mtRNA in lymphatic cancer: Bibliographic review

Nereis. Interdisciplinary Ibero-American Journal of Methods, Modelling and Simulation., 2021
Lymphoma is the most common type of blood cancer today and, as its name suggests, it begins in the lymphatic system. The origin of this disease is related to mitochondrial defects, generated by mutations in the PNPase enzyme or polynucleotide ...
Myriam Lizanda Piqueras, Ignacio Ventura González   +1 more
semanticscholar   +6 more sources

Crystal structure of dimeric human PNPase reveals why disease-linked mutants suffer from low RNA import and degradation activities [PDF]

Nucleic Acids Research, 2018
Human polynucleotide phosphorylase (PNPase) is an evolutionarily conserved 3′-to-5′ exoribonuclease principally located in mitochondria where it is responsible for RNA turnover and import.
Chia-liang Lin, Wei-zen Yang, Hanna S Yuan   +2 more
exaly   +3 more sources

Mitochondrial sequencing identifies long noncoding RNA features that promote binding to PNPase.

Am J Physiol Cell Physiol
Extranuclear localization of long non-coding RNAs (lncRNAs) is poorly understood. Based on machine learning evaluations, we propose a lncRNA-mitochondrial interaction pathway where Polynucleotide Phosphorylase (PNPase), through domains that provide ...
Taylor AD, Hathaway QA, Kunovac A, Pinti MV, Newman MS, Cook CC, Cramer ER, Starcovic SA, Winters MT, Westemeier-Rice ES, Fink GK, Durr AJ, Rizwan S, Shepherd DL, Robart AR, Martinez I, Hollander JM.   +16 more
europepmc   +4 more sources

The small RNA SraG participates in PNPase homeostasis [PDF]

RNA, 2016
The rpsO-pnp operon encodes ribosomal protein S15 and polynucleotide phosphorylase, a major 3′–5′ exoribonuclease involved in mRNA decay in Escherichia coli. The gene for the SraG small RNA is located between the coding regions of the rpsO and pnp genes,
Fanette Fontaine, Elise Gasiorowski, C. Gracia, Mathieu Ballouche, J. Caillet, A. Marchais, E. Hajnsdorf   +6 more
semanticscholar   +5 more sources

A cooperative PNPase-Hfq-RNA carrier complex facilitates bacterial riboregulation [PDF]

Molecular Cell, 2020
Polynucleotide phosphorylase (PNPase) is an ancient exoribonuclease conserved in the course of evolution and is found in species as diverse as bacteria and humans. Paradoxically, Escherichia coli PNPase can act not only as an RNA degrading enzyme but also by an unknown mechanism as a chaperone for small regulatory RNAs (sRNAs), with pleiotropic ...
T. Dendooven, Dhriti Sinha, Alžběta Roeselová, Todd A. Cameron, Nicholas R. De Lay, B. Luisi, K. Bandyra   +6 more
semanticscholar   +7 more sources

Identification of Polynucleotide Phosphorylase (PNPase) in Escherichia coli Involved in Persister Formation [PDF]

bioRxiv, 2018
Despite the identification of many genes and pathways involved in the persistence phenomenon of bacteria, the mechanisms of persistence are not well understood.
Nan Wu, Yumeng Zhang, Shanshan Zhang, Youhua Yuan, Shuang Liu, P. Cui, Wen Zhang, Ying Zhang   +7 more
semanticscholar   +4 more sources