Results 21 to 30 of about 283,934 (290)

A kinetic ruler controls mRNA poly(A) tail length. [PDF]

Genes Dev
Poly(A) tails of newly synthesized mRNAs have uniform lengths, arising through cooperation between the cleavage and polyadenylation complex (CPAC) and poly(A) binding proteins (PABPs). In the budding yeast Saccharomyces cerevisiae , the responsible PABP is the evolutionarily conserved CCCH ...
Gabs E, Aalto-Setälä E, Välisaari A, Malinen AM, Jensen TH, McLaughlin SH, Passmore LA, Turtola M.   +7 more
europepmc   +3 more sources

Polyadenylated tail length variation pattern in ultra-rapid vitrified bovine oocytes [PDF]

Veterinary World, 2016
Aim: The current study aims at investigating the polyadenylated (poly[A]) tail length of morphologically high and low competent oocytes at different developmental stages.
D. J. Dutta, Himangshu Raj, Hiramoni Dev
doaj   +1 more source

Tales of Detailed Poly(A) Tails [PDF]

Trends in Cell Biology, 2019
Poly(A) tails are non-templated additions of adenosines at the 3' ends of most eukaryotic mRNAs. In the nucleus, these RNAs are co-transcriptionally cleaved at a poly(A) site and then polyadenylated before being exported to the cytoplasm. In the cytoplasm, poly(A) tails play pivotal roles in the translation and stability of the mRNA.
Nicholson, Angela L, Pasquinelli, Amy E
openaire   +4 more sources

Characterization of the Role of Hexamer AGUAAA and Poly(A) Tail in Coronavirus Polyadenylation. [PDF]

PLoS ONE, 2016
Similar to eukaryotic mRNA, the positive-strand coronavirus genome of ~30 kilobases is 5'-capped and 3'-polyadenylated. It has been demonstrated that the length of the coronaviral poly(A) tail is not static but regulated during infection; however, little
Yu-Hui Peng, Ching-Houng Lin, Chao-Nan Lin, Chen-Yu Lo, Tsung-Lin Tsai, Hung-Yi Wu   +5 more
doaj   +1 more source

East of EDEN was a poly(A) tail [PDF]

Biology of the Cell, 2003
AbstractPost‐transcriptional regulations of gene expression (control of mRNA stability and translation) play a central role in achieving cellular functions. In a large number of cases, post‐transcriptional regulations are dependent on mRNA poly(A) tails, as mRNAs with a long poly(A) tail are generally much more stable and actively translated than ...
Paillard, Luc, Osborne, H Beverley
openaire   +3 more sources

Evaluation of Stem-Loop Reverse Transcription and Poly-A Tail Extension in MicroRNA Analysis of Body Fluids [PDF]

, 2014
MicroRNA has been demonstrated to be a viable tool for body fluid identification purposes in forensic casework. Stem-loop reverse transcription (slRT) is regularly used for cDNA synthesis from mature miRNA, along with poly-A tail extension.
Dunnett, Hannah, Van der Meer, Dieudonne, Williams, Graham   +2 more
core   +1 more source

Poly(A) Tail Shortening by a Mammalian Poly(A)-specific 3′-Exoribonuclease [PDF]

Journal of Biological Chemistry, 1997
3'-Exonucleolytic removal of the poly(A) tail is the first and often rate-limiting step in the decay of many eucaryotic mRNAs. In a cytoplasmic extract from HeLa cells, the poly(A) tail of mRNA was degraded from the 3'-end. In agreement with earlier in vivo observations, prominent decay intermediates differed in length by about 30 nucleotides. The Mg2+-
C G, Körner, E, Wahle
openaire   +2 more sources

Nanopore direct RNA sequencing maps the complexity of Arabidopsis mRNA processing and m6A modification [PDF]

, 2020
Understanding genome organization and gene regulation requires insight into RNA transcription, processing and modification. We adapted nanopore direct RNA sequencing to examine RNA from a wild-type accession of the model plant Arabidopsis thaliana and a ...
Barton, Geoffrey J., Gould, Peter D., Hall, Anthony J. W., Knop, Katarzyna, Mackinnon, Katarzyna, Parker, Matthew T., Schurch, Nicholas J., Sherwood, Anna V., Simpson, Gordon G.   +8 more
core   +3 more sources

Poly(A)-tail profiling reveals an embryonic switch in translational control [PDF]

, 2014
Poly(A) tails enhance the stability and translation of most eukaryotic mRNAs, but difficulties in globally measuring poly(A)-tail lengths have impeded greater understanding of poly(A)-tail function.
Bartel, David P., Chen, Grace R., Eichhorn, Stephen W., Sive, Hazel, Subtelny, Alexander O.   +4 more
core   +1 more source

RNA-binding proteins distinguish between similar sequence motifs to promote targeted deadenylation by Ccr4-Not

eLife, 2019
The Ccr4-Not complex removes mRNA poly(A) tails to regulate eukaryotic mRNA stability and translation. RNA-binding proteins contribute to specificity by interacting with both Ccr4-Not and target mRNAs, but this is not fully understood.
Michael W Webster, James AW Stowell, Lori A Passmore   +2 more
doaj   +1 more source