Results 41 to 50 of about 114,953,758 (241)
Poly(a) selection introduces bias and undue noise in direct RNA-sequencing
BMC Genomics, 2022 Background Genome-wide RNA-sequencing technologies are increasingly critical to a wide variety of diagnostic and research applications. RNA-seq users often first enrich for mRNA, with the most popular enrichment method being poly(A) selection.
Marcus J. Viscardi, Joshua A. Arribere
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Molecular Oncology, EarlyView.This study identifies somatostatin receptor 4 (Sstr4) as a critical tumor suppressor against skin and head/neck cancers (HNSCC, cSCC, and BCC). The loss of Sstr4 removes a check on cell growth, causing hyperactivation of the MAPK‐ERK signaling pathway (↑).
Ali Taqvi +6 more
wiley +1 more source
Synthesis of a poly(A) tail from poly(A) tail-lacking DI RNA.
, 2016 (A) Left panel: hexamer AGUAAA-containing DI RNA constructs with a poly(A) tail or other non-poly(A) sequences immediately downstream of the 3’UTR. Right panel: sequences of the 3’ end of DI RNA at the indicated times.
Ching-Houng Lin (3228993) +5 more
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Molecular Oncology, EarlyView.In head and neck squamous cell carcinoma (HNSCC) p53 and p63 exert opposite roles on the transcription regulation of the lncRNA NEAT1. Under basal conditions, p53 levels are low and p63 represses NEAT1 expression. Upon genotoxic stress, p53 is rapidly induced, displacing p63 from the NEAT1 promoter leading to NEAT1 transcriptional activation and ...
Sara De Domenico +5 more
wiley +1 more source
Determination of the minimum poly(A) tail length for the initiation of poly(A) tail lengthening.
, 2016 (A) DI RNA constructs with various poly(A) tail lengths. (B) Poly(A) tail lengths for DI RNA constructs at different times, as determined by sequencing the RT-PCR products shown in S2 Fig.
Ching-Houng Lin (3228993) +5 more
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Characteristics of biter and victim piglets apparent before a tail biting outbreak [PDF]
, 2011 Little is known about the characteristics of biters and victims before the appearance of a tail-biting outbreak in groups of pigs. This study aimed to characterise biters and victims (according to gender and performance) and to quantify their behavioural
Kemp, B. +5 more
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Pre- and postovulatory aging of murine oocytes affect the transcript level and poly(A) tail length of maternal effect genes. [PDF]
PLoS ONE, 2014 Maternal effect genes code for oocyte proteins that are important for early embryogenesis. Transcription in oocytes does not take place from the onset of meiotic progression until zygotic genome activation.
Debora Dankert +7 more
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Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA
FEBS Open Bio, EarlyView.PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro +1 more
wiley +1 more source
MARK4 enhances stress granule formation under oxidative stress and increases tau accumulation
FEBS Open Bio, EarlyView.MARK4 (red dots) localizes to stress granules (orange dots) and promotes their formation under oxidative stress by modulating TIA1 (blue dots). MARK4 and TIA1 synergistically increase tau (purple) accumulation, and the reduction of the TIA1 ortholog suppresses neurodegeneration in a fly model.
Sho Nakajima +8 more
wiley +1 more source
Identification of factors regulating poly(A) tail synthesis and maturation [PDF]
, 2009 Posttranscriptional maturation of the 3' end of eukaryotic pre-mRNAs occurs as a three-step pathway involving site-specific cleavage, polymerization of a poly(A) tail, and trimming of the newly synthesized tail to its mature length.
Smith, Mandy M. +3 more
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