Results 31 to 40 of about 123,331 (250)

Presence of poly(A) and poly(A)-rich tails in a positive-strand RNA virus known to lack 3׳ poly(A) tails

Virology, 2014
Here we show that Tobacco mosaic virus (TMV), a positive-strand RNA virus known to end with 3׳ tRNA-like structures, does possess a small fraction of gRNA bearing polyadenylate tails. Particularly, many tails are at sites corresponding to the 3׳ end of near full length gRNA, and are composed of poly(A)-rich sequences containing the other nucleotides in
Li, Weimin, Zhang, Yongqiang, Zhang, Chao, Pei, Xinwu, Wang, Zhixing, Jia, Shirong   +5 more
openaire   +2 more sources

Control of translation and miRNA-dependent repression by a novel poly(A) binding protein, hnRNP-Q. [PDF]

PLoS Biology, 2013
Translation control often operates via remodeling of messenger ribonucleoprotein particles. The poly(A) binding protein (PABP) simultaneously interacts with the 3' poly(A) tail of the mRNA and the eukaryotic translation initiation factor 4G (eIF4G) to ...
Yuri V Svitkin, Akiko Yanagiya, Alexey E Karetnikov, Tommy Alain, Marc R Fabian, Arkady Khoutorsky, Sandra Perreault, Ivan Topisirovic, Nahum Sonenberg   +8 more
doaj   +1 more source

An engineered surrogate poly(A) tail to wag translation initiation. [PDF]

Cell Res
Lohse S, Weber W.
europepmc   +4 more sources

Saltatory Forward Movement of a Poly(A) Polymerase during Poly(A) Tail Addition [PDF]

Journal of Biological Chemistry, 2007
Vaccinia poly(A) polymerase (VP55) interacts with > or = 33-nucleotide (nt) primers via uridylates at two sites (-27/-26 and -10). It adds approximately 30-nt poly(A) tails with a rapid, processive burst in which the first few nt are added without substantial primer movement, and addition of the remaining adenylates is dependent upon a six-uridylate ...
Janice M, Yoshizawa, Changzheng, Li, Paul D, Gershon   +2 more
openaire   +2 more sources

mRNA poly(A)-tail changes specified by deadenylation broadly reshape translation in Drosophila oocytes and early embryos

eLife, 2016
Because maturing oocytes and early embryos lack appreciable transcription, posttranscriptional regulatory processes control their development. To better understand this control, we profiled translational efficiencies and poly(A)-tail lengths throughout ...
Stephen W Eichhorn, Alexander O Subtelny, Iva Kronja, Jamie C Kwasnieski, Terry L Orr-Weaver, David P Bartel   +5 more
doaj   +1 more source

Poly(a) selection introduces bias and undue noise in direct RNA-sequencing

BMC Genomics, 2022
Background Genome-wide RNA-sequencing technologies are increasingly critical to a wide variety of diagnostic and research applications. RNA-seq users often first enrich for mRNA, with the most popular enrichment method being poly(A) selection.
Marcus J. Viscardi, Joshua A. Arribere
doaj   +1 more source

A kingdom-wide full-length RNA atlas reveals the evolutionary landscapes of plant poly(A) tails

Genome Biology
Background Poly(A) tails play crucial roles in regulating mRNA degradation and translation, yet their evolutionary dynamics remain largely unexplored. Results Here, we generate a comprehensive full-length RNA atlas of poly(A) tail based on 130 million ...
Yang Chen, Wenqin Lu, Hai He, Lihao Zheng, Xuehua Pan, Ting Sun, Fengjiao Bu, Yuhan Fang, Yufei Sun, Yanping Long, Meina Wang, Shengdan Wu, Lin Liu, Jinbu Jia, Yuannian Jiao, Zhangli Hu, Yu Zhang, Jixian Zhai   +17 more
doaj   +1 more source

Biochemical and in silico identification of the active site and the catalytic mechanism of the circadian deadenylase HESPERIN

FEBS Open Bio, 2022
The 24‐h molecular clock is based on the stability of rhythmically expressed transcripts. The shortening of the poly(A) tail of mRNAs is often the first and rate‐limiting step that determines the lifespan of a mRNA and is catalyzed by deadenylases ...
Rafailia A. A. Beta, Athanasios Kyritsis, Veroniki Douka, Eirini Papanastasi, Marianna Rizouli, Demetres D. Leonidas, Dimitrios Vlachakis, Nikolaos A. A. Balatsos   +7 more
doaj   +1 more source

TRAIL‐PEG‐Apt‐PLGA nanosystem as an aptamer‐targeted drug delivery system potential for triple‐negative breast cancer therapy using in vivo mouse model

Molecular Oncology, EarlyView.
Aptamers are used both therapeutically and as targeting agents in cancer treatment. We developed an aptamer‐targeted PLGA–TRAIL nanosystem that exhibited superior therapeutic efficacy in NOD/SCID breast cancer models. This nanosystem represents a novel biotechnological drug candidate for suppressing resistance development in breast cancer.
Gulen Melike Demirbolat, Aslihan Kucuk, Omer Erdogan, Samed Ozer, Bensu Kozan, Tugba Cuceli, Erkan Gumus, Evrim Cevik, Ozge Cevik   +8 more
wiley   +1 more source

Establishment of a humanized patient‐derived xenograft mouse model of high‐grade serous ovarian cancer for preclinical evaluation of combination immunotherapy

Molecular Oncology, EarlyView.
We have established a humanized orthotopic patient‐derived xenograft (Hu‐oPDX) mouse model of high‐grade serous ovarian cancer (HGSOC) that recapitulates human tumor–immune interactions. Using combined anti‐PD‐L1/anti‐CD73 immunotherapy, we demonstrate the model's improved biological relevance and enhanced translational value for preclinical ...
Luka Tandaric, Line Bjørge, Martine Rott Lode, Cecilie Fredvik Torkildsen, Pia Aehnlich, Rammah Elnour, Daniela Elena Costea, Lars Andreas Akslen, Liv Cecilie Vestrheim Thomsen, Emmet McCormack, Katrin Kleinmanns   +10 more
wiley   +1 more source