Poly(A)-tail profiling reveals an embryonic switch in translational control [PDF]
Nature, 2014 Poly(A) tails enhance the stability and translation of most eukaryotic mRNAs, but difficulties in globally measuring poly(A)-tail lengths have impeded greater understanding of poly(A)-tail function.
Bartel, David P. +4 more
core +3 more sources
RNA binding protein Caprin-2 is a pivotal regulator of the central osmotic defense response [PDF]
eLife, 2015 In response to an osmotic challenge, the synthesis of the antidiuretic hormone arginine vasopressin (AVP) increases in the hypothalamus, and this is accompanied by extension of the 3′ poly(A) tail of the AVP mRNA, and the up-regulation of the expression ...
Agnieszka Konopacka +4 more
doaj +5 more sources
Loop structure in poly(A) tail of mRNA vaccine enhances antigen translation efficiency and mRNA stability. [PDF]
NPJ VaccinesAs mRNA technology emerges as a groundbreaking innovation, an increasing number of studies are attempting to increase expression levels by optimizing the structure of mRNA, such as the 5’ or 3’ untranslated regions (UTR).
Oh A +18 more
europepmc +2 more sources
A kinetic ruler controls mRNA poly(A) tail length. [PDF]
Genes DevPoly(A) tails of newly synthesized mRNAs have uniform lengths, arising through cooperation between the cleavage and polyadenylation complex (CPAC) and poly(A) binding proteins (PABPs). In the budding yeast Saccharomyces cerevisiae , the responsible PABP is the evolutionarily conserved CCCH ...
Gabs E +7 more
europepmc +3 more sources
Molecular Basis for poly(A) RNP Architecture and Recognition by the Pan2-Pan3 Deadenylase
Cell, 2019 The stability of eukaryotic mRNAs is dependent on a ribonucleoprotein (RNP) complex of poly(A)-binding proteins (PABPC1/Pab1) organized on the poly(A) tail.
Conti, E. +6 more
core +2 more sources
MARTRE family proteins negatively regulate CCR4-NOT activity to protect poly(A) tail length and promote translation of maternal mRNA. [PDF]
Nat CommunThe mammalian early embryo development requires translation of maternal mRNA inherited from the oocyte. While poly(A) tail length influences mRNA translation efficiency during the oocyte-to-embryo transition (OET), molecular mechanisms regulating ...
Yang J +8 more
europepmc +2 more sources
The molecular basis of coupling between poly(A)-tail length and translational efficiency. [PDF]
Elife, 2021 In animal oocytes and early embryos, mRNA poly(A)-tail length strongly influences translational efficiency (TE), but later in development this coupling between tail length and TE disappears.
Xiang K, Bartel DP.
europepmc +2 more sources
PAL-AI reveals genetic determinants that control poly(A)-tail length during oocyte maturation, with relevance to human fertility. [PDF]
Nat CommunIn oocytes of mammals and other animals, gene regulation is mediated primarily through changes in poly(A)-tail length. Here, we introduce PAL-AI, an integrated neural network machine-learning model that accurately predicts tail-length changes in maturing
Xiang K, Bartel DP.
europepmc +2 more sources
TAIL-seq: Genome-wide Determination of Poly(A) Tail Length and 3′ End Modifications [PDF]
Molecular Cell, 2014 Global investigation of the 3' extremity of mRNA (3'-terminome), despite its importance in gene regulation, has not been feasible due to technical challenges associated with homopolymeric sequences and relative paucity of mRNA. We here develop a method, TAIL-seq, to sequence the very end of mRNA molecules.
Chang, Hyeshik +3 more
openaire +4 more sources
Poly (A) tail length of human mitochondrial mRNAs is tissue-specific and a mutation in LRPPRC results in transcript-specific patterns of deadenylation. [PDF]
Mol Genet Metab Rep, 2020 Mutations in LRPPRC cause Leigh Syndrome French Canadian (LSFC), an early onset neurodegenerative disease, with differential tissue involvement. The molecular basis for tissue specificity in this disease remains unknown.
Honarmand S, Shoubridge EA.
europepmc +2 more sources