Measuring the tail: Methods for poly(A) tail profiling. [PDF]
Wiley Interdiscip Rev RNA, 2023 AbstractThe 3′‐end poly(A) tail is an important and potent feature of most mRNA molecules that affects mRNA fate and translation efficiency. Polyadenylation is a posttranscriptional process that occurs in the nucleus by canonical poly(A) polymerases (PAPs).
Brouze A +3 more
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Cytoplasmic deadenylase Ccr4 is required for translational repression of LRG1 mRNA in the stationary phase. [PDF]
PLoS ONE, 2017 Ccr4 is a major cytoplasmic deadenylase involved in mRNA poly(A) tail shortening in Saccharomyces cerevisiae. We have previously shown that Ccr4 negatively regulates expression of LRG1 mRNA encoding a GTPase-activating protein for the small GTPase Rho1 ...
Duong Long Duy +2 more
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Genome-Wide Analysis of PAPS1-Dependent Polyadenylation Identifies Novel Roles for Functionally Specialized Poly(A) Polymerases in Arabidopsis thaliana. [PDF]
PLoS Genetics, 2015 The poly(A) tail at 3' ends of eukaryotic mRNAs promotes their nuclear export, stability and translational efficiency, and changes in its length can strongly impact gene expression.
Christian Kappel +9 more
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Integrative analysis of Poly(A)-seq and RNA-seq reveals transcriptional regulation of poly(A) tail length in tuberculosis. [PDF]
Microbiol SpectrTuberculosis (TB) is a serious global health problem, and the molecular mechanisms underlying TB pathogenesis remain poorly understood to date. Differential expression of many genes is an important contributor to TB, and poly(A) tails have previously ...
Tuohetaerbaike B +10 more
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The Dynamic Poly(A) Tail Acts as a Signal Hub in mRNA Metabolism. [PDF]
Cells, 2023 In eukaryotes, mRNA metabolism requires a sophisticated signaling system. Recent studies have suggested that polyadenylate tail may play a vital role in such a system.
Zhang G, Luo H, Li X, Hu Z, Wang Q.
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Poly(A) tail dynamics: Measuring polyadenylation, deadenylation and poly(A) tail length. [PDF]
Methods Enzymol, 2021 Transcription of mRNAs culminates in RNA cleavage and a coordinated polyadenylation event at the 3' end. In its journey to be translated, the resulting transcript is under constant regulation by cap-binding proteins, miRNAs, and RNA binding proteins, including poly(A) binding proteins (PABPs).
Murphy MR, Doymaz A, Kleiman FE.
europepmc +3 more sources
Preserving the Poly(A) Tail: Strategies Viruses Use to 'CYA' (Cover Your A's). [PDF]
VirusesThe poly(A) tail on viral mRNAs plays an important role in gene expression, given the role of the 3′ mRNA tail in mRNA stability and translation. Viruses have developed several strategies to maintain the integrity of their poly(A) tails.
Wilusz J.
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Poly(A) tail regulation in stem cells and early development. [PDF]
Cell RegenEukaryotic mRNAs are polyadenylated at their 3’-ends, and the poly(A) tails play critical roles in post-transcriptional gene regulation by influencing mRNA stability and translation.
Miao X, Hu G.
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Regulation of Coronaviral Poly(A) Tail Length during Infection [PDF]
PLoS ONE, 2013 The positive-strand coronavirus genome of ~30 kilobase in length and subgenomic (sg) mRNAs of shorter lengths, are 5' and 3'-co-terminal by virtue of a common 5'-capped leader and a common 3'-polyadenylated untranslated region. Here, by ligating head-to-tail viral RNAs from bovine coronavirus-infected cells and sequencing across the ligated junctions ...
Hung-Yi Wu +3 more
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A Synthetic Poly(A) Tail Targeting Extracellular CIRP Inhibits Sepsis. [PDF]
J Immunol, 2023 Abstract Sepsis is an infectious inflammatory disease that often results in acute lung injury (ALI). Cold-inducible RNA-binding protein (CIRP) is an intracellular RNA chaperon that binds to mRNA’s poly(A) tail. However, CIRP can be released in sepsis, and extracellular CIRP (eCIRP) is a damage-associated molecular pattern, exaggerating ...
Murao A +5 more
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