Measuring the tail: Methods for poly(A) tail profiling [PDF]
Wiley Interdisciplinary Reviews RNA, 2023 AbstractThe 3′‐end poly(A) tail is an important and potent feature of most mRNA molecules that affects mRNA fate and translation efficiency. Polyadenylation is a posttranscriptional process that occurs in the nucleus by canonical poly(A) polymerases (PAPs).
Aleksandra Brouze +2 more
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Poly(A) tail regulation in stem cells and early development [PDF]
Cell RegenerationEukaryotic mRNAs are polyadenylated at their 3’-ends, and the poly(A) tails play critical roles in post-transcriptional gene regulation by influencing mRNA stability and translation.
Xiaosu Miao, Guang Hu
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Integrative analysis of Poly(A)-seq and RNA-seq reveals transcriptional regulation of poly(A) tail length in tuberculosis [PDF]
Microbiology SpectrumTuberculosis (TB) is a serious global health problem, and the molecular mechanisms underlying TB pathogenesis remain poorly understood to date. Differential expression of many genes is an important contributor to TB, and poly(A) tails have previously ...
Bahetibieke Tuohetaerbaike +10 more
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Poly(A) tail dynamics: Measuring polyadenylation, deadenylation and poly(A) tail length. [PDF]
Methods Enzymol, 2021 Transcription of mRNAs culminates in RNA cleavage and a coordinated polyadenylation event at the 3' end. In its journey to be translated, the resulting transcript is under constant regulation by cap-binding proteins, miRNAs, and RNA binding proteins, including poly(A) binding proteins (PABPs).
Murphy MR, Doymaz A, Kleiman FE.
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TENT5C extends Odf1 poly(A) tail to sustain sperm morphogenesis and fertility [PDF]
Nature CommunicationsChanges in the poly(A) tail length of Odf1 and other transcripts critical for male fertility have been linked to translational activation during sperm formation1–3.
Marine Baptissart +6 more
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The Dynamic Poly(A) Tail Acts as a Signal Hub in mRNA Metabolism [PDF]
Cells, 2023 In eukaryotes, mRNA metabolism requires a sophisticated signaling system. Recent studies have suggested that polyadenylate tail may play a vital role in such a system.
Guiying Zhang +4 more
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Preserving the Poly(A) Tail: Strategies Viruses Use to ‘CYA’ (Cover Your A’s) [PDF]
VirusesThe poly(A) tail on viral mRNAs plays an important role in gene expression, given the role of the 3′ mRNA tail in mRNA stability and translation. Viruses have developed several strategies to maintain the integrity of their poly(A) tails.
Jeffrey Wilusz
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Loop structure in poly(A) tail of mRNA vaccine enhances antigen translation efficiency and mRNA stability [PDF]
npj VaccinesAs mRNA technology emerges as a groundbreaking innovation, an increasing number of studies are attempting to increase expression levels by optimizing the structure of mRNA, such as the 5’ or 3’ untranslated regions (UTR).
Ayoung Oh +18 more
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Torpor-Induced Regulation of Poly(A) Tail Machinery in 13-Lined Ground Squirrel Brown Adipose Tissue [PDF]
Journal of Developmental BiologyThe poly(A) tail has long been known to play a central role in mRNA stability, storage, and translational competence, making it a potential key regulator during hypometabolic states. During seasonal torpor, hibernating mammals must frequently enter these
Saif Rehman +2 more
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Frontiers in Microbiology, 2018 The duck hepatitis A virus type 1 (DHAV-1) is a member of Picornaviridae family, the genome of the virus contains a 5′ untranslated region (5′ UTR), a large open reading frame that encodes a polyprotein precursor and a 3′ UTR followed by a poly(A) tail ...
Jun-Hao Chen +18 more
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