Results 161 to 170 of about 152,403 (204)

A radioisotopic assay for polyamine oxidase

Analytical Biochemistry, 1990
A new radioisotopic assay for polyamine oxidase with N1-acetylspermine as substrate is presented. A modified method for the chemical synthesis of radioactive N1-acetylspermine, which gave a good yield, is also described. The reaction mixture, containing N1-[14C]acetylspermine and tissue homogenate, was incubated for the enzyme reaction and applied to a
T, Kumazawa, H, Seno, O, Suzuki
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Cytotoxicity of polyamines to Amoeba proteus: Role of polyamine oxidase

Cell Biology and Toxicology, 1996
It has been shown that oxidation of polyamines by polyamine oxidases can produce toxic compounds (H2O2, aldehydes, ammonia) and that the polyamine oxidase-polyamine system is implicated, in vitro, in the death of several parasites. Using Amoeba proteus as an in vitro model, we studied the cytotoxicity to these cells of spermine, spermidine, their ...
Schenkel, Eric, Dubois, Jacques, Helson, Marianne, Hanocq, Michel   +3 more
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Inhibition and killing of fungi by the polyamine oxidase-polyamine system

Antonie van Leeuwenhoek, 1990
Both components of the polyamine oxidase (PAO)-polyamine system are known to be present in phagocytes and have thus been postulated to contribute to the antimicrobial activity of these cells. Therefore, the effects of the PAO-polyamine system on three medically important opportunistic fungi were examined.
S M, Levitz, D J, DiBenedetto, R D, Diamond   +2 more
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Occurrence of polyamine oxidase in tissues of the catfish

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1987
1. Polyamine oxidase (PAO) activity was found in the brain, intestine, kidney and liver of the siluroid catfish using N1-acetylspermine as substrate. It was highest in the intestine and lowest in the brain. 2. Substrate specificity of the enzyme was tested in the intestine and liver and the highest activity was found with N1-acetylspermine, followed by
T, Kumazawa, O, Suzuki
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Polyamine oxidase in higher plants

Biochemical and Biophysical Research Communications, 1970
Abstract A polyamine-specific oxidase found in the leaves of barley and maize seedlings oxidises spermine optimally at pH 4.5, the products being hydrogen peroxide, 1-(3-aminopropyl) pyrroline and 1,3-diaminopropane. Spermidine and 3,3′-diaminodipropylamine were oxidised more slowly than spermine with an optimum at about pH 7.0.
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The Di- and Polyamine Oxidases of Plants

1988
Although the di- and polyamine oxidases of plants are apparently limited in their distribution, in some species they are remarkably active. Earlier work on these enzymes has been summarized by Smith (1985a), Morgan (1985), Rinaldi et al., (1986), and Mondovi and Avigliano (1987).
T A, Smith, J H, Barker
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Amino oxidase amperometric biosensor for polyamines

Journal of Electroanalytical Chemistry and Interfacial Electrochemistry, 1991
Abstract An improved amino oxidase enzyme electrode has been constructed and applied to the determination of the amount of polyamines present in real samples. The electrode is based on the amperometric detection of H 2 O 2 produced in the enzymatic oxidation of polyamines by amino oxidase.
GASPARINI R., SCARPA M., DI PAOLO M. L., STEVANATO, Roberto, RIGO A.   +4 more
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Inhibition of the respiratory burst of human neutrophils by the polyamine oxidase-polyamine system

International Journal of Immunopharmacology, 1986
The addition of the polyamines, spermine and spermidine, to human neutrophils caused a depression of the hexose-monophosphate (HMP) shunt activity of neutrophils stimulated with latex particles but not of unstimulated cells. The effect was dependent on the presence of bovine serum and was not observed when normal human serum was substituted for bovine ...
A, Ferrante, G M, Maxwell, V O, Rencis, A C, Allison, D M, Morgan   +4 more
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Polyamines, polyamine oxidases and nitric oxide in development, abiotic and biotic stresses

Plant Science, 2011
Nitric oxide (NO), polyamines (PAs), diamine oxidases (DAO) and polyamine oxidases (PAO) play important roles in wide spectrum of physiological processes such as germination, root development, flowering and senescence and in defence responses against abiotic and biotic stress conditions.
Rinukshi, Wimalasekera, Felix, Tebartz, Günther F E, Scherer   +2 more
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Polyamine oxidase and tissue transglutaminase activation in rat small intestine by polyamines

Biochimica et Biophysica Acta (BBA) - General Subjects, 1999
Polyamine degradation was studied in the small intestine from rats fed on a polyamine-supplemented diet. Lactalbumin diet was given to Hooded-Lister rats, with or without 5 mg rat(-1) day(-1) of putrescine or spermidine for 5 days. Polyamine oxidase activity increased with putrescine and spermidine in the diet, whereas spermidine/spermine N(1 ...
P, Tunici, A, Sessa, E, Rabellotti, G, Grant, S, Bardocz, A, Perin   +5 more
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