Results 51 to 60 of about 31,758 (236)

Synthetic biology—putting engineering into biology [PDF]

, 2006
Synthetic biology is interpreted as the engineering-driven building of increasingly complex biological entities for novel applications. Encouraged by progress in the design of artificial gene networks, de novo DNA synthesis and protein engineering, we ...
Heinemann, Matthias,, Panke, Sven,
core   +1 more source

Biosynthesis of polyketide synthase extender units [PDF]

Nat. Prod. Rep., 2009
This review covers the biosynthesis of extender units that are utilized for the assembly of polyketides by polyketide synthases. The metabolic origins of each of the currently known polyketide synthase extender units are covered.
Yolande A, Chan, Angela M, Podevels, Brian M, Kevany, Michael G, Thomas   +3 more
openaire   +2 more sources

Investigating A Multi-Domain Polyketide Synthase in Amphidinium carterae

Marine Drugs, 2023
Dinoflagellates are unicellular organisms that are implicated in harmful algal blooms (HABs) caused by potent toxins that are produced through polyketide synthase (PKS) pathways.
Saddef Haq, Benjamin L. Oyler, Ernest Williams, Mohd M. Khan, David R. Goodlett, Tsvetan Bachvaroff, Allen R. Place   +6 more
doaj   +1 more source

Reprogramming of the antimycin NRPS-PKS assembly lines inspired by gene evolution

Nature Communications, 2018
Modifying the non-ribosomal peptide synthase (NRPS)/polyketide synthase (PKS) pathway to generate novel non-ribosomal peptides often results in a loss of productivity.
Takayoshi Awakawa, Takuma Fujioka, Lihan Zhang, Shotaro Hoshino, Zhijuan Hu, Junko Hashimoto, Ikuko Kozone, Haruo Ikeda, Kazuo Shin-Ya, Wen Liu, Ikuro Abe   +10 more
doaj   +1 more source

Biosynthesis of coelulatin for the methylation of anthraquinone featuring HemN-like radical S-adenosyl-L-methionine enzyme

Frontiers in Microbiology, 2022
Bacterial aromatic polyketides are usually biosynthesized by the type II polyketide synthase (PKS-II) system. Advances in deoxyribonucleic acid (DNA) sequencing, informatics, and biotechnologies have broadened opportunities for the discovery of aromatic ...
Lishuang Nie, Tianyi Wei, Mingming Cao, Yunbin Lyu, Shaochen Wang, Zhiyang Feng   +5 more
doaj   +1 more source

Quantification of Fusarium graminearum and Fusarium culmorum by real-time PCR system and zearalenone assessment in maize [PDF]

, 2012
Zearalenone (ZEA) is a mycotoxin produced by some species of Fusarium, especially by Fusarium graminearum and F. culmorum. ZEA induces hyperoestrogenic responses in mammals and can result in reproductive disorders in farm animals. In the present study, a
Atoui, Ali, El Khoury, André, Kallassy, Mireille, Lebrihi, Ahmed   +3 more
core   +1 more source

The Transcription Factor FgSge1 Harnesses the SAGA Complex to Activate Mycotoxin Biosynthesis and Fungal Virulence

Advanced Science, EarlyView.
This study reveals the molecular mechanism by which the transcription factor FgSge1 regulates mycotoxin biosynthesis and virulence in Fusarium graminearum. FgSge1 binds to the TAARGTTT cis‐element, enabling self‐activation. It recruits the SAGA complex, promotes histone acetylation, and facilitates jet‐like chromatin remodeling, thereby activating ...
Yueqi Zhang, Jingrui Wang, Qiaowan Chen, Chen Jiao, Yun Chen, Zhongshou Wu, Zhonghua Ma   +6 more
wiley   +1 more source

Cryptic polyketide synthase genes in non-pathogenic Clostridium SPP. [PDF]

PLoS ONE, 2012
Modular type I polyketide synthases (PKS) produce a vast array of bacterial metabolites with highly diverse biological functions. Notably, all known polyketides were isolated from aerobic bacteria, and yet no example has been reported for strict ...
Swantje Behnken, Christian Hertweck
doaj   +1 more source

Systematic Engineering of Proteases in Saccharopolyspora Spinosa Reveals Synergistic Enhancement of Spinosad Biosynthesis via Substrate Flux Optimization

Advanced Science, EarlyView.
ARTP mutagenesis yielded Saccharopolyspora spinosa mutant D184 with improved extracellular nitrogen utilization. An integrated workflow of protease genetic manipulation, multi‐omics, and rational synergy design pinpointed a pepP‐clpP‐htpX synergistic triangular combination.
Duo Jin, Wangqiong Chen, Danlu Yang, Qing Liu, Baolong Bai, Zirui Dai, Xirong Liu, Zirong Zhu, Jie Rang, Liqiu Xia   +9 more
wiley   +1 more source

The malonyl/acetyl-transferase from murine fatty acid synthase is a promiscuous engineering tool for editing polyketide scaffolds

Communications Chemistry
Modular polyketide synthases (PKSs) play a vital role in the biosynthesis of complex natural products with pharmaceutically relevant properties. Their modular architecture makes them an attractive target for engineering to produce platform chemicals and ...
Lynn Buyachuihan, Simon Reiners, Yue Zhao, Martin Grininger   +3 more
doaj   +1 more source