Results 31 to 40 of about 21,069 (236)

Quantification of N-acetylcysteamine activated methylmalonate incorporation into polyketide biosynthesis

Beilstein Journal of Organic Chemistry, 2013
Polyketides are biosynthesized through consecutive decarboxylative Claisen condensations between a carboxylic acid and differently substituted malonic acid thioesters, both tethered to the giant polyketide synthase enzymes.
Stephan Klopries, Uschi Sundermann, Frank Schulz   +2 more
doaj   +1 more source

Investigation of carbonyl amidation and O-methylation during biosynthesis of the pharmacophore pyridyl of antitumor piericidins

Synthetic and Systems Biotechnology, 2022
Piericidins are a large family of bacterial α-pyridone antibiotics with antitumor activities such as their anti-renal carcinoma activity exhibited recently in nude mice.
Wanlu Li, Wenyu Zhang, Yijia Cheng, Yaoyao Shen, Jianzhao Qi, Hou-Wen Lin, Yongjun Zhou   +6 more
doaj   +1 more source

Chemoinformatic-guided engineering of polyketide synthases [PDF]

Journal of the American Chemical Society, 2019
Abstract Polyketide synthase (PKS) engineering is an attractive method to generate new molecules such as commodity, fine and specialty chemicals. A significant challenge in PKS design is engineering a partially reductive module to produce a saturated β-carbon through a reductive loop exchange.
Amin Zargar, Ravi Lal, Luis Valencia, Jessica Wang, Tyler William H. Backman, Pablo Cruz-Morales, Ankita Kothari, Miranda Werts, Andrew R. Wong, Constance B. Bailey, Arthur Loubat, Yuzhong Liu, Yan Chen, Samantha Chang, Veronica T. Benites, Amanda C. Hernández, Jesus F. Barajas, Mitchell G. Thompson, Carolina Barcelos, Rasha Anayah, Hector Garcia Martin, Aindrila Mukhopadhyay, Christopher J. Petzold, Edward E. K. Baidoo, Leonard Katz, Jay D. Keasling   +25 more
openaire   +4 more sources

The role of the phosphopantetheinyltransferase enzyme, PswP, in the biosynthesis of antimicrobial secondary metabolites by Serratia marcescens Db10 [PDF]

, 2014
Phosphopantetheinyl transferase (PPTase) enzymes fulfil essential roles in primary and secondary metabolism in prokaryotes, archaea and eukaryotes. PPTase enzymes catalyse the essential modification of the carrier protein domain of fatty acid synthases ...
Gerc, Amy J., Stanley-Wall, Nicola R.; id_orcid, Coulthurst, Sarah J.; id_orcid   +2 more
core   +1 more source

A Wagner–Meerwein‐Like Rearrangement Generates a Vinyl Group in the Biosynthesis of the Polychlorinated Lipopeptides Fischerazoles

Angewandte Chemie, EarlyView.
The fischerazoles, unusual cyanobacterial chlorinated lipopeptides are reported. During their biosynthesis, a linear fatty acyl‐acyl carrier protein (ACP) precursor is rearranged through the action of the S‐adenosyl‐methionine (SAM)‐dependent methyltransferase FshF, to generate a pendant vinyl group.
Sandra A. C. Figueiredo, Kathleen Abt, Konrad Viehrig, Peter J. Jervis, Teresa P. Martins, Iñaki Lacomba, Chuhan Li, Daniele Castagnolo, Pedro N. Leão   +8 more
wiley   +2 more sources

Manipulating polyketide stereochemistry by exchange of polyketide synthase modules

Chemical Communications, 2020
Exchange of polyketide synthase (PKS) modules by genetic engineering leads to efficient modification of polyketide stereochemistry.
Massicard, Jean-Malo, Soligot, Claire, Weissman, Kira J., Jacob, Christophe   +3 more
openaire   +4 more sources

Engineering Broader Specificity into an Antibiotic-Producing Polyketide Synthase [PDF]

, 1998
The wide-specificity loading module for the avermectin-producing polyketide synthase was grafted onto the first multienzyme component (DEBS1) of the erythromycin-producing polyketide synthase in place of the normal loading module. Expression
Jesús Cortés, Barrie Wilkinson, Andrew F. A. Marsden, James Staunton, Peter F. Leadlay, Nicholas J. Dunster   +5 more
core   +1 more source

Structural control of polyketide formation in plant-specific polyketide synthases [PDF]

Chemistry & Biology, 2000
Polyketide synthases (PKSs) generate molecular diversity by utilizing different starter molecules and by controlling the final length of the polyketide. Although exploitation of this mechanistic variability has produced novel polyketides, the structural foundation of this versatility is unclear. Plant-specific PKSs are essential for the biosynthesis of
Jez, Joseph M, Austin, Michael B, Ferrer, Jean-Luc, Bowman, Marianne E, Schröder, Joachim, Noel, Joseph P   +5 more
openaire   +2 more sources

Evidence for an iterative module in chain elongation on the azalomycin polyketide synthase

Beilstein Journal of Organic Chemistry, 2016
The assembly-line synthases that produce bacterial polyketide natural products follow a modular paradigm in which each round of chain extension is catalysed by a different set or module of enzymes.
Hui Hong, Yuhui Sun, Yongjun Zhou, Emily Stephens, Markiyan Samborskyy, Peter F. Leadlay   +5 more
doaj   +1 more source

A Polyketide Synthase Gene, ACRTS2, Is Responsible for Biosynthesis of Host-Selective ACR-Toxin in the Rough Lemon Pathotype of Alternaria alternata

Molecular Plant-Microbe Interactions, 2012
The rough lemon pathotype of Alternaria alternata produces host-selective ACR-toxin and causes Alternaria leaf spot disease of rough lemon (Citrus jambhiri). The structure of ACR-toxin I (MW = 496) consists of a polyketide with an α-dihydropyrone ring in
Y. Izumi, K. Ohtani, Y. Miyamoto, A. Masunaka, T. Fukumoto, K. Gomi, Y. Tada, K. Ichimura, T. L. Peever, K. Akimitsu   +9 more
doaj   +1 more source