Results 11 to 20 of about 186,140 (231)

Polyketide synthesis in vitro on a modular polyketide synthase [PDF]

Chemistry & Biology, 1995
The 6-deoxyerythronolide B synthase (DEBS) of Saccharopolyspora erythraea, which synthesizes the aglycone core of the antibiotic erythromycin A, contains some 30 active sites distributed between three multienzyme polypeptides (designated DEBS1-3). This complexity has hitherto frustrated mechanistic analysis of such enzymes.
Wiesmann, Kirsten E.H., Cortés, Jesus, Brown, Murray J.B., Cutter, Annabel L., Staunton, James, Leadlay, Peter F.   +5 more
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The polyketide backbone of thiolactomycin is assembled by an unusual iterative polyketide synthase [PDF]

Chemical Communications, 2017
Thiotetronate polyketide assembly by an unusual iterative synthase is reconstructed via in vitro enzymology and chemical probes.
Marie E. Yurkovich, Robert Jenkins, Yuhui Sun, Manuela Tosin, Peter F. Leadlay   +4 more
openaire   +5 more sources

Acyltransferases as Tools for Polyketide Synthase Engineering [PDF]

Antibiotics, 2018
Polyketides belong to the most valuable natural products, including diverse bioactive compounds, such as antibiotics, anticancer drugs, antifungal agents, immunosuppressants and others.
Ewa Maria Musiol-Kroll, Wolfgang Wohlleben   +1 more
doaj   +3 more sources

Diversifying Hybrid Polyketide Synthases through the Rapamycin Polyketide Synthase

Polyketide synthases are large protein complexes that make products called polyketides. Many of these products are medicines like the antibiotic erythromycin and the immunosuppressant agent rapamycin. These synthases are composed of sequential subunits called modules.
Jaegy, Hubert
openaire   +2 more sources

Identification of 2-Aminothiazole-4-Carboxylate Derivatives Active against Mycobacterium tuberculosis H37Rv and the beta-Ketoacyl-ACP Synthase mtFabH [PDF]

, 2009
Background: Tuberculosis (TB) is a disease which kills two million people every year and infects approximately over one-third of the world's population.
Johnston, Blair F., Coxon, Geoffrey D., Geoffrey D Coxon, Besra, Gurdyal S., Nahoum G Anthony, Gillespie, Stephen, Amani Alnimr, Al-Balas Qosay, Todd, MH, Coxon, GD, Gillespie Stephen H., McHugh, Timothy D., Bilal Al-Jaidi, Besra, GS, Johnston, BF, Mackay, Simon, Alnimr, Amani, Taylor, Rebecca C., Mackay Simon P., Blair F Johnston, Qosay Al-Balas, Alnimr, A, Coxon, Geoffrey, Brown, Alistair K., Simon P Mackay, Besra Gurdyal S., Mackay, SP, Taylor, RC, Gillespie, Stephen H., Johnston, Blair, Brown, Alistair, Al-Jaidi, Bilal, Al-Jaidi Bilal, McHugh, TD, Brown, AK, Anthony, Nahoum, Besra, Gurdyal; id_orcid, Besra, Gurdyal, Timothy D McHugh, McHugh, Timothy, Taylor, Rebecca, Rebecca C Taylor, Coxon Geoffrey D., Stephen H Gillespie, Alistair K Brown, Al-Balas, Q, Al-Balas, Qosay, Grainne Abbott, Abbott, Grainne, Gurdyal S Besra, Anthony, NG, Alnimr Amani, Anthony, Nahoum G., Johnston Blair F., Anthony Nahoum G., Mackay, Simon P., Taylor Rebecca C., McHugh Timothy D., Gillespie, SH, Brown Alistair K., Abbott, G, Abbott Grainne, Al-Jaidi, B   +62 more
core   +5 more sources

Structure of a modular polyketide synthase [PDF]

Nature, 2014
Polyketide natural products constitute a broad class of compounds with diverse structural features and biological activities. Their biosynthetic machinery, represented by type I polyketide synthases (PKSs), has an architecture in which successive modules catalyse two-carbon linear extensions and keto-group processing reactions on intermediates ...
Dutta, Somnath, Whicher, Jonathan R., Hansen, Douglas A., Hale, Wendi A., Chemler, Joseph A., Congdon, Grady R., Narayan, Alison R., Håkansson, Kristina, Sherman, David H., Smith, Janet L., Skiniotis, Georgios   +10 more
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Synthetic biology of polyketide synthases [PDF]

Journal of Industrial Microbiology and Biotechnology, 2018
Abstract Complex reduced polyketides represent the largest class of natural products that have applications in medicine, agriculture, and animal health. This structurally diverse class of compounds shares a common methodology of biosynthesis employing modular enzyme systems called polyketide synthases (PKSs).
Yuzawa, Satoshi, Backman, Tyler WH, Keasling, Jay D, Katz, Leonard   +3 more
openaire   +4 more sources

Cloning and characterization of novel methylsalicylic acid synthase gene involved in the biosynthesis of isoasperlactone and asperlactone in Aspergillus westerdijkiae [PDF]

, 2009
Aspergillus westerdijkiae is the main producer of several biologically active polyketide metabolites including isoasperlactone and asperlactone. A 5298 bp polyketide synthase gene ‘‘aomsas” has been cloned in Aspergillus westerdijkiae by using gene ...
Atoui, Ali, Dobson, Alan D. W., Mathieu, Florence, Puel, Olivier, Dao, Huy Phong, Liboz, Thierry, O’Callaghan, John, O'Callaghan, John, Bacha, Nafees, Dobson, Alan D.W., Lebrihi, Ahmed   +10 more
core   +1 more source

The insect pathogen Serratia marcescens Db10 uses a hybrid non-ribosomal peptide synthetase-polyketide synthase to produce the antibiotic althiomycin [PDF]

, 2012
There is a continuing need to discover new bioactive natural products, such as antibiotics, in genetically-amenable micro-organisms. We observed that the enteric insect pathogen, Serratia marcescens Db10, produced a diffusible compound that inhibited the
Coulthurst Sarah J., Challis Gregory L., Stanley-Wall, Nicola R; id_orcid, Gregory L Challis, Gerc, Amy J., Amy J. Gerc, Gerc Amy J., Nicola R Stanley-Wall, Gregory L. Challis (136140), Song Lijiang, Coulthurst, Sarah J; id_orcid, Sarah J. Coulthurst, Lijiang Song (136135), Lijiang Song, Stanley-Wall, Nicola R., Stanley-Wall Nicola R., Coulthurst, Sarah J., Nicola R. Stanley-wall, Challis, Gregory L., Challis, Gregory L, Amy J Gerc, Sarah J. Coulthurst (136148), Song, Lijiang, Gerc, Amy J, Amy J. Gerc, Amy J. Gerc (136131), Nicola R. Stanley-Wall (136144), Sarah J Coulthurst, Gregory L. Challis   +28 more
core   +2 more sources

Engineering Polyketide Synthases [PDF]

The Scientific World JOURNAL, 2002
Complex polyketides, exemplified by the molecules shown in Fig. 1, are produced mainly from a group of mycelia-forming bacteria belonging to the actinomycete family and have applications in medicine or agriculture. Worldwide sales of polyketides exceed $15 B annually.
openaire   +3 more sources