Novel Aspects of Polynucleotide Phosphorylase Function in Streptomyces [PDF]
Antibiotics, 2018 Polynucleotide phosphorylase (PNPase) is a 3′–5′-exoribnuclease that is found in most bacteria and in some eukaryotic organelles. The enzyme plays a key role in RNA decay in these systems.
George H. Jones
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Polynucleotide phosphorylase protects against renal tubular injury via blocking mt-dsRNA-PKR-eIF2α axis [PDF]
Nature Communications, 2023 Renal tubular atrophy is a hallmark of chronic kidney disease. The study by Zhu et al. reveals the protective role of tubular polynucleotide phosphorylase (PNPT1) against renal atrophy by blocking the leakage of mitochondrial dsRNAs into cytoplasm where ...
Yujie Zhu +12 more
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Polynucleotide Phosphorylase Mediates a New Mechanism of Persister Formation in Escherichia coli [PDF]
Microbiology Spectrum, 2023 Despite the identification of many genes and pathways involved in the persistence phenomenon in bacteria, the mechanisms of persistence are not well understood.
Nan Wu +8 more
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Pseudomonas aeruginosa Polynucleotide Phosphorylase Contributes to Ciprofloxacin Resistance by Regulating PrtR [PDF]
Frontiers in Microbiology, 2019 Pseudomonas aeruginosa is an opportunistic bacterial pathogen that causes various acute and chronic infections. It is intrinsically resistant to a variety of antibiotics. However, production of pyocins during SOS response sensitizes P.
Zheng Fan +11 more
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Rapid and scalable detection of synthetic mRNA byproducts using polynucleotide phosphorylase and polythymidine oligonucleotides [PDF]
RNA BiologyProduction and storage of synthetic mRNA can introduce a variety of byproducts which reduce the overall integrity and functionality of mRNA vaccines and therapeutics.
Francis Combes +3 more
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Polynucleotide phosphorylase: Not merely an RNase but a pivotal post-transcriptional regulator. [PDF]
PLoS Genetics, 2018 Almost 60 years ago, Severo Ochoa was awarded the Nobel Prize in Physiology or Medicine for his discovery of the enzymatic synthesis of RNA by polynucleotide phosphorylase (PNPase).
Todd A Cameron +2 more
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Polynucleotide phosphorylase is implicated in homologous recombination and DNA repair in Escherichia coli [PDF]
BMC Microbiology, 2017 Background Polynucleotide phosphorylase (PNPase, encoded by pnp) is generally thought of as an enzyme dedicated to RNA metabolism. The pleiotropic effects of PNPase deficiency is imputed to altered processing and turnover of mRNAs and small RNAs, which ...
Thomas Carzaniga +3 more
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Defects in Polynucleotide phosphorylase impairs virulence in Escherichia coli O157:H7 [PDF]
Frontiers in Microbiology, 2015 Polynucleotide phosphorylase (PNPase) is reported to regulate virulence in Salmonella, Yersinia spp. and Campylobacter jejuni, yet its role in Escherichia coli O157:H7 has not been investigated. To gain insights into its roles in E.
Jia eHu, Jia eHu, Meijun eZhu
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Polynucleotide phosphorylase regulates multiple virulence factors and the stabilities of small RNAs RsmY/Z in Pseudomonas aeruginosa [PDF]
Frontiers in Microbiology, 2016 Post-transcriptional regulation enables bacteria to quickly response to environmental stresses. Polynucleotide phosphorylase (PNPase), which contains an N-terminal catalytic core and C-terminal RNA binding KH-S1 domains, is involved in RNA processing ...
Ronghao eChen +10 more
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The exoribonuclease Polynucleotide Phosphorylase influences the virulence and stress responses of yersiniae and many other pathogens [PDF]
Frontiers in Cellular and Infection Microbiology, 2013 Microbes are incessantly challenged by both biotic and abiotic stressors threatening their existence. Therefore, bacterial pathogens must possess mechanisms to successfully subvert host immune defenses as well as overcome the stress associated with host ...
Jason A. Rosenzweig, Ashok K Chopra
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