Polynucleotide phosphorylase regulates multiple virulence factors and the stabilities of small RNAs RsmY/Z in Pseudomonas aeruginosa [PDF]
Frontiers in Microbiology, 2016 Post-transcriptional regulation enables bacteria to quickly response to environmental stresses. Polynucleotide phosphorylase (PNPase), which contains an N-terminal catalytic core and C-terminal RNA binding KH-S1 domains, is involved in RNA processing ...
Ronghao eChen +10 more
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The exoribonuclease Polynucleotide Phosphorylase influences the virulence and stress responses of yersiniae and many other pathogens [PDF]
Frontiers in Cellular and Infection Microbiology, 2013 Microbes are incessantly challenged by both biotic and abiotic stressors threatening their existence. Therefore, bacterial pathogens must possess mechanisms to successfully subvert host immune defenses as well as overcome the stress associated with host ...
Jason A. Rosenzweig, Ashok K Chopra
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The Protein Interaction of RNA Helicase B (RhlB) and Polynucleotide Phosphorylase (PNPase) Contributes to the Homeostatic Control of Cysteine in Escherichia coli. [PDF]
J Biol Chem, 2015 PNPase, one of the major enzymes with 3′ to 5′ single-stranded RNA degradation and processing activities, can interact with the RNA helicase RhlB independently of RNA degradosome formation in Escherichia coli.
Tseng YT +3 more
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Structure and mechanism of Mycobacterium smegmatis polynucleotide phosphorylase. [PDF]
RNA, 2021 Polynucleotide phosphorylase (PNPase) catalyzes stepwise phosphorolysis of the 3′-terminal phosphodiesters of RNA chains to yield nucleoside diphosphate products.
Unciuleac MC +4 more
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Activity and Function in Human Cells of the Evolutionary Conserved Exonuclease Polynucleotide Phosphorylase. [PDF]
Int J Mol Sci, 2022 Polynucleotide phosphorylase (PNPase) is a phosphorolytic RNA exonuclease highly conserved throughout evolution. Human PNPase (hPNPase) is located in mitochondria and is essential for mitochondrial function and homeostasis. Not surprisingly, mutations in
Falchi FA, Pizzoccheri R, Briani F.
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Polynucleotide phosphorylase and RNA helicase CshA cooperate in Bacillus subtilis mRNA decay. [PDF]
RNA Biol, 2021 Polynucleotide phosphorylase (PNPase), a 3ʹ exoribonuclease that degrades RNA in the 3ʹ-to-5ʹ direction, is the major mRNA decay activity in Bacillus subtilis.
Ingle S +5 more
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Pseudomonas aeruginosa Polynucleotide Phosphorylase Controls Tolerance to Aminoglycoside Antibiotics by Regulating the MexXY Multidrug Efflux Pump. [PDF]
Antimicrob Agents Chemother, 2021 Pseudomonas aeruginosa is an opportunistic pathogen that shows high intrinsic resistance to a variety of antibiotics. The MexX-MexY-OprM efflux pump plays an important role in bacterial resistance to aminoglycoside antibiotics.
Fan Z +9 more
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Selective 8-oxo-rG stalling occurs in the catalytic core of polynucleotide phosphorylase (PNPase) during degradation. [PDF]
Proc Natl Acad Sci U S ASignificance Polynucleotide phosphorylase (PNPase) is a widely conserved exoribonuclease that degrades single-stranded RNA transcripts in the 3′ to 5′ direction, forming single-nucleotide diphosphates and a short oligonucleotide product.
Miller LG +14 more
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Functions of Conserved Domains of Human Polynucleotide Phosphorylase on RNA Oxidation. [PDF]
Insights Biomed Res, 2019 Human polynucleotide phosphorylase (hPNPase), an exoribonuclease that is primarily localized in mitochondria, plays an important role in reducing oxidized RNA and protecting cells under oxidative stress conditions. hPNPase contains two catalytic domains (
Malla S, Li Z.
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Polynucleotide phosphorylase promotes the stability and function of Hfq-binding sRNAs by degrading target mRNA-derived fragments. [PDF]
Nucleic Acids Res, 2019 In many Gram-negative and some Gram-positive bacteria, small regulatory RNAs (sRNAs) that bind the RNA chaperone Hfq have a pivotal role in modulating virulence, stress responses, metabolism and biofilm formation.
Cameron TA, Matz LM, Sinha D, De Lay NR.
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