Rapid and scalable detection of synthetic mRNA byproducts using polynucleotide phosphorylase and polythymidine oligonucleotides [PDF]
RNA BiologyProduction and storage of synthetic mRNA can introduce a variety of byproducts which reduce the overall integrity and functionality of mRNA vaccines and therapeutics.
Francis Combes +3 more
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Study on the structure-function relationship of polynucleotide phosphorylase: model of a proteolytic degraded polynucleotide phosphorylase [PDF]
Nucleic Acids Research, 1976 It is already known that modification of E. coli polynucleotide phosphorylase by endogenous proteolysis induces drastic changes in both phosphorolysis and polymerisation reactions. The structural parameters of the proteolysed polynucleotide phosphorylase are described.
Annie Guissani, Claude Portier
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Polynucleotide phosphorylase regulates multiple virulence factors and the stabilities of small RNAs RsmY/Z in Pseudomonas aeruginosa [PDF]
Frontiers in Microbiology, 2016 Post-transcriptional regulation enables bacteria to quickly response to environmental stresses. Polynucleotide phosphorylase (PNPase), which contains an N-terminal catalytic core and C-terminal RNA binding KH-S1 domains, is involved in RNA processing ...
Ronghao eChen +10 more
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The exoribonuclease Polynucleotide Phosphorylase influences the virulence and stress responses of yersiniae and many other pathogens [PDF]
Frontiers in Cellular and Infection Microbiology, 2013 Microbes are incessantly challenged by both biotic and abiotic stressors threatening their existence. Therefore, bacterial pathogens must possess mechanisms to successfully subvert host immune defenses as well as overcome the stress associated with host ...
Jason A. Rosenzweig, Ashok K Chopra
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Polynucleotide phosphorylase exonuclease and polymerase activities on single-stranded DNA ends are modulated by RecN, SsbA and RecA proteins [PDF]
, 2011 Bacillus subtilis pnpA gene product, polynucleotide phosphorylase (PNPase), is involved in double-strand break (DSB) repair via homologous recombination (HR) or non-homologous end-joining (NHEJ).
Paula P. Cárdenas +6 more
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STUDIES ON POLYNUCLEOTIDES SYNTHESIZED BY POLYNUCLEOTIDE PHOSPHORYLASE [PDF]
Journal of Biological Chemistry, 1957 Leon Heppel +2 more
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Localization of components of the RNA-degrading machine in Bacillus subtilis [PDF]
Frontiers in Microbiology, 2016 In bacteria, the control of mRNA stability is crucial to allow rapid adaptation to changing conditions. In most bacteria, RNA degradation is catalyzed by the RNA degradosome, a protein complex composed of endo- and exoribonucleases, RNA helicases and ...
Nora Cascante-Estepa +2 more
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Oligonucleotides as Primers for Polynucleotide Phosphorylase
Journal of Biological Chemistry, 1960 Maxine Singer, Leon Heppel, R.J. Hilmoe
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Insoluble derivatives of polynucleotide‐phosphorylase [PDF]
FEBS Letters, 1973 Jeremy C. Smith +3 more
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Polynucleotide Phosphorylase of Micrococcus lysodeikticus
Journal of Biological Chemistry, 1963 Maxine Singer, Barbara O’Brien
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