Identification of genes potentially regulated by human polynucleotide phosphorylase (hPNPase old-35) using melanoma as a model. [PDF]
PLoS ONE, 2013 Human Polynucleotide Phosphorylase (hPNPase(old-35) or PNPT1) is an evolutionarily conserved 3'→ 5' exoribonuclease implicated in the regulation of numerous physiological processes including maintenance of mitochondrial homeostasis, mtRNA import and ...
Upneet K Sokhi +8 more
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Polynucleotide phosphorylase has an impact on cell biology of Campylobacter jejuni [PDF]
Frontiers in Cellular and Infection Microbiology, 2012 Polynucleotide phosphorylase, encoded by the pnp gene, is known to degrade mRNA, mediating post-transcriptional regulation and may affect cellular functions. The role of PNPase is pleiotropic.
Nabila eHaddad +12 more
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Crystal structure of Caulobacter crescentus polynucleotide phosphorylase reveals a mechanism of RNA substrate channelling and RNA degradosome assembly [PDF]
Open Biology, 2012 Polynucleotide phosphorylase (PNPase) is an exoribonuclease that cleaves single-stranded RNA substrates with 3′–5′ directionality and processive behaviour.
Steven W. Hardwick +4 more
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The Protein Interaction of RNA Helicase B (RhlB) and Polynucleotide Phosphorylase (PNPase) Contributes to the Homeostatic Control of Cysteine in Escherichia coli. [PDF]
J Biol Chem, 2015 PNPase, one of the major enzymes with 3′ to 5′ single-stranded RNA degradation and processing activities, can interact with the RNA helicase RhlB independently of RNA degradosome formation in Escherichia coli.
Tseng YT +3 more
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Structure and mechanism of Mycobacterium smegmatis polynucleotide phosphorylase. [PDF]
RNA, 2021 Polynucleotide phosphorylase (PNPase) catalyzes stepwise phosphorolysis of the 3′-terminal phosphodiesters of RNA chains to yield nucleoside diphosphate products.
Unciuleac MC +4 more
europepmc +2 more sources
Activity and Function in Human Cells of the Evolutionary Conserved Exonuclease Polynucleotide Phosphorylase. [PDF]
Int J Mol Sci, 2022 Polynucleotide phosphorylase (PNPase) is a phosphorolytic RNA exonuclease highly conserved throughout evolution. Human PNPase (hPNPase) is located in mitochondria and is essential for mitochondrial function and homeostasis. Not surprisingly, mutations in
Falchi FA, Pizzoccheri R, Briani F.
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Polynucleotide phosphorylase and RNA helicase CshA cooperate in Bacillus subtilis mRNA decay. [PDF]
RNA Biol, 2021 Polynucleotide phosphorylase (PNPase), a 3ʹ exoribonuclease that degrades RNA in the 3ʹ-to-5ʹ direction, is the major mRNA decay activity in Bacillus subtilis.
Ingle S +5 more
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Pseudomonas aeruginosa Polynucleotide Phosphorylase Controls Tolerance to Aminoglycoside Antibiotics by Regulating the MexXY Multidrug Efflux Pump. [PDF]
Antimicrob Agents Chemother, 2021 Pseudomonas aeruginosa is an opportunistic pathogen that shows high intrinsic resistance to a variety of antibiotics. The MexX-MexY-OprM efflux pump plays an important role in bacterial resistance to aminoglycoside antibiotics.
Fan Z +9 more
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Distinctive effects of domain deletions on the manganese-dependent DNA polymerase and DNA phosphorylase activities of Mycobacterium smegmatis polynucleotide phosphorylase. [PDF]
Biochemistry, 2013 Polynucleotide phosphorylase (PNPase) plays synthetic and degradative roles in bacterial RNA metabolism; it is also suggested to participate in bacterial DNA transactions.
Unciuleac MC, Shuman S.
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Selective 8-oxo-rG stalling occurs in the catalytic core of polynucleotide phosphorylase (PNPase) during degradation. [PDF]
Proc Natl Acad Sci U S ASignificance Polynucleotide phosphorylase (PNPase) is a widely conserved exoribonuclease that degrades single-stranded RNA transcripts in the 3′ to 5′ direction, forming single-nucleotide diphosphates and a short oligonucleotide product.
Miller LG +14 more
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