Activity and Function in Human Cells of the Evolutionary Conserved Exonuclease Polynucleotide Phosphorylase. [PDF]
Int J Mol Sci, 2022 Polynucleotide phosphorylase (PNPase) is a phosphorolytic RNA exonuclease highly conserved throughout evolution. Human PNPase (hPNPase) is located in mitochondria and is essential for mitochondrial function and homeostasis. Not surprisingly, mutations in
Falchi FA, Pizzoccheri R, Briani F.
europepmc +2 more sources
Structure and mechanism of <i>Mycobacterium smegmatis</i> polynucleotide phosphorylase. [PDF]
RNA, 2021 Polynucleotide phosphorylase (PNPase) catalyzes stepwise phosphorolysis of the 3′-terminal phosphodiesters of RNA chains to yield nucleoside diphosphate products.
Unciuleac MC +4 more
europepmc +2 more sources
Pseudomonas aeruginosa Polynucleotide Phosphorylase Controls Tolerance to Aminoglycoside Antibiotics by Regulating the MexXY Multidrug Efflux Pump. [PDF]
Antimicrob Agents Chemother, 2021 Pseudomonas aeruginosa is an opportunistic pathogen that shows high intrinsic resistance to a variety of antibiotics. The MexX-MexY-OprM efflux pump plays an important role in bacterial resistance to aminoglycoside antibiotics.
Fan Z +9 more
europepmc +2 more sources
Crystal structure of Caulobacter crescentus polynucleotide phosphorylase reveals a mechanism of RNA substrate channelling and RNA degradosome assembly [PDF]
Open Biology, 2012 Polynucleotide phosphorylase (PNPase) is an exoribonuclease that cleaves single-stranded RNA substrates with 3′–5′ directionality and processive behaviour.
Steven W. Hardwick +4 more
doaj +3 more sources
Polynucleotide phosphorylase promotes the stability and function of Hfq-binding sRNAs by degrading target mRNA-derived fragments. [PDF]
Nucleic Acids Res, 2019 In many Gram-negative and some Gram-positive bacteria, small regulatory RNAs (sRNAs) that bind the RNA chaperone Hfq have a pivotal role in modulating virulence, stress responses, metabolism and biofilm formation.
Cameron TA, Matz LM, Sinha D, De Lay NR.
europepmc +2 more sources
Global analysis of mRNA decay intermediates in Bacillus subtilis wild-type and polynucleotide phosphorylase-deletion strains. [PDF]
Mol Microbiol, 2014 Liu B +5 more
europepmc +4 more sources
CvfA protein and polynucleotide phosphorylase act in an opposing manner to regulate Staphylococcus aureus virulence. [PDF]
J Biol Chem, 2014 Numata S +4 more
europepmc +4 more sources
Localization of components of the RNA-degrading machine in Bacillus subtilis [PDF]
Frontiers in Microbiology, 2016 In bacteria, the control of mRNA stability is crucial to allow rapid adaptation to changing conditions. In most bacteria, RNA degradation is catalyzed by the RNA degradosome, a protein complex composed of endo- and exoribonucleases, RNA helicases and ...
Nora Cascante-Estepa +2 more
doaj +3 more sources
Cell Reports, 2015 Oocyte factors not only drive somatic cell nuclear transfer reprogramming but also augment the efficiency and quality of induced pluripotent stem cell (iPSC) reprogramming.
Swea-Ling Khaw +4 more
doaj +2 more sources
Exploring the mitochondrial microRNA import pathway through Polynucleotide Phosphorylase (PNPase). [PDF]
J Mol Cell Cardiol, 2017 Shepherd DL +9 more
europepmc +2 more sources