The endoribonucleolytic N-terminal half of Escherichia coli RNase E is evolutionarily conserved in Synechocystis sp. and other bacteria but not the C-terminal half, which is sufficient for degradosome assembly [PDF]
, 1998 Escherichia coli RNase E, an essential single-stranded specific endoribonuclease, is required for both ribosomal RNA processing and the rapid degradation of mRNA.
Jakobsen, J.S. +5 more
core +2 more sources
Polynucleotide Phosphorylase from a Cyanobacterium (Synechococcus sp.): Subunit Composition and Properties [PDF]
, 1982 Polynucleotide phosphorylase from cells of the cyanobacterium Synechococcus sp. has been purified 1400-fold by an improved procedure. The enzyme purified to homogeneity and lacking nuclease, phosphatase and protease contaminations reveals a single band ...
Nolden, W.-T., Richter, G.
core +2 more sources
Microorganisms, 2021 Escherichia coli C is a strong biofilm producer in comparison to E. coli K-12 laboratory strains due to higher expression of the pgaABCD operon encoding the enzymes for the biosynthesis of the extracellular polysaccharide poly-β-1,6-N-acetylglucosamine ...
Thomas Carzaniga +5 more
doaj +1 more source
A Blue Native-PAGE analysis of membrane protein complexes in Clostridium thermocellum [PDF]
, 2011 Background Clostridium thermocellum is a Gram-positive thermophilic anaerobic bacterium with the unusual capacity to convert cellulosic biomass into ethanol and hydrogen. Identification and characterization of protein complexes in C.
Fan, Keqiang +6 more
core +3 more sources
Scientific Reports, 2023 A mutant deficient in polynucleotide phosphorylase (PNPase) activity was previously constructed in Enterococcus faecalis 14; a strain producing a leaderless two-peptide enterocin DD14 (EntDD14).
Rabia Ladjouzi +3 more
doaj +1 more source
Human polynucleotide phosphorylase (hPNPase(old-35)): an evolutionary conserved gene with an expanding repertoire of RNA degradation functions. [PDF]
Oncogene, 2011 Das SK +6 more
europepmc +3 more sources
Enzymatic Synthesis and Fractionation of Fluorescent PolyU RNAs
Bio-Protocol, 2018 The physical properties of viral-length polyuridine (PolyU) RNAs, which cannot base-pair and form secondary structures, are compared with those of normal-composition RNAs, composed of comparable numbers of each of A, U, G and C nucleobases.
Christian Beren +4 more
doaj +1 more source
Mitochondrial Genome Engineering: The Revolution May Not Be CRISPR-Ized. [PDF]
, 2018 In recent years mitochondrial DNA (mtDNA) has transitioned to greater prominence across diverse areas of biology and medicine. The recognition of mitochondria as a major biochemical hub, contributions of mitochondrial dysfunction to various diseases, and
Gammage, Payam A +2 more
core +1 more source
Insights into Xanthomonas axonopodis pv. citri biofilm through proteomics [PDF]
, 2013 Background: Xanthomonas axonopodis pv. citri (X. a. pv. citri) causes citrus canker that can result in defoliation and premature fruit drop with significant production losses worldwide. Biofilm formation is an important process in bacterial pathogens and
Garavaglia, Betiana Soledad +6 more
core +1 more source
RNase E biomolecular condensates stimulate PNPase activity
Scientific Reports, 2023 Bacterial Ribonucleoprotein bodies (BR-bodies) play an essential role in organizing RNA degradation via phase separation in the cytoplasm of bacteria. BR-bodies mediate multi-step mRNA decay through the concerted activity of the endoribonuclease RNase E ...
Michael J. Collins +4 more
doaj +1 more source