Results 11 to 20 of about 1,913 (163)

Raman Optical Activity as a Tool to Monitor Polynucleotide Conformation in Solutions. [PDF]

Chemistry
Raman optical activity (ROA) studies of nucleic acids have been hindered by limited computational tools to link the spectra to the structure. The study shows that accurate density functional simulations are possible for model nucleotides, bringing more information about molecular geometry and dynamics in solutions.
Para Kkadan MS, Kapitán J, Kessler J, Zapletal O, Andrushchenko V, Bouř P.   +5 more
europepmc   +2 more sources

Ear keloids: An innovative 3-steps combined treatment.

Skin Res Technol, 2023
Abstract Introduction An excessive proliferation of fibroblasts and collagen synthesis after an injury may lead to a benign fibrous tumor, known as keloid, which does not regress spontaneously. Earlobes are a very frequent site of onset, since after a trauma (i.e., piercing) keloids may develop either on the helix and on the anterior or posterior lobe,
Domenico P, Giuliana C, Daniele B, Bruno B, Alessandro G, Fabrizio M, Matteo TC, Claudio C.   +7 more
europepmc   +2 more sources

Structural and functional characterization of the cytotoxic protein ledodin, an atypical ribosome-inactivating protein from shiitake mushroom (Lentinula edodes). [PDF]

Protein Sci, 2023
Abstract We have purified ledodin, a cytotoxic 22‐kDa protein from shiitake mushroom (Lentinula edodes) consisting of a 197 amino acid chain. Ledodin possessed N‐glycosylase activity on the sarcin‐ricin loop of mammalian 28S rRNA and inhibited protein synthesis. However, it was not active against insect, fungal, and bacterial ribosomes. In vitro and in
Citores L, Ragucci S, Russo R, Gay CC, Chambery A, Di Maro A, Iglesias R, Ferreras JM.   +7 more
europepmc   +2 more sources

Polyribonuceleotide synthesis by cytoplasmic enzymes [PDF]

, 1965
The possibility that the cell cytoplasm contains enzymes catalysing RNA biosynthesis was investigated in Landschutz ascites tumour cells. Enzyme fractions were obtained by differential centrifugation of disrupted cell homogenates.
Wykes, J. R
core   +7 more sources

Evidence for a new transition in polyribonucleotides from raman optical activity [PDF]

, 1999
Vibrational Raman optical activity (ROA) measurements are reported on single-stranded poly(rA) and double-stranded poly(rA)·poly(rU) in the temperature range 2–45 °C.
Turpin, P.Y., Shvedko, A.G., Otto, Cornelis, Greve, J., Ermolenkov, V.V., Kruglik, S.G., Boudart, B., Bell, Alasdair F., Lefebvre, N., Barron, Laurence D., Gaquière, Christophe, Hecht, Lutz, Constant, M., Lorriaux, A.   +13 more
core   +2 more sources

Observation on cadavers and through ultrasonography using a 2 mm needle length for intradermal injections

Skin Research and Technology, Volume 29, Issue 11, November 2023., 2023
Abstract Background An intradermal injection is a medical procedure that involves administering a small amount of medication or substance into the dermal layer of the skin. This research focused on identifying the most suitable injection needle for precise intradermal administration of skin boosters.
Kyu‐Ho Yi, Brian Lee, Michael James Kim, Seo‐Hyun Lee, Inneke Jane Hidajat, Ting Song Lim, Hyoung Moon Kim, Jin‐Hyun Kim   +7 more
wiley   +1 more source

A Novel Pathway of Functional microRNA Uptake and Mitochondria Delivery

Advanced Science, Volume 10, Issue 24, August 25, 2023., 2023
In the presence of serum, microRNAs (miRNAs) spontaneously form ≈110 nm nanoparticles, mediated bycationic proteins. When depleted, miRNA uptake is blocked. Unlike lysosome‐located, nanoparticle‐delivere mechanism are preferentially delivered to mitochondria of recipient cells by PNPT1.
Jiachen Liu, Weili Li, Jianfeng Li, Eli Song, Hongwei Liang, Weiwei Rong, Xinli Jiang, Nuo Xu, Wei Wang, Shuang Qu, Shouyong Gu, Yujing Zhang, Chen‐ Yu Zhang, Ke Zen   +13 more
wiley   +1 more source

Downregulation of LRP/LR with siRNA inhibits several cancer hallmarks in lung cancer cells

FEBS Open Bio, Volume 13, Issue 2, Page 323-340, February 2023., 2023
LRP/LR is overexpressed in many cancer cell types and is associated with various cancer‐promoting processes. In this study, we downregulated LRP/LR in lung cancer cells and showed that this strategy has a four‐fold function in impeding key cancer hallmarks in the lung cancer cells, which therefore has the potential for improving the prognosis of those ...
Monique J. Bignoux, Tyrone C. Otgaar, Martin Bernert, Stefan F. T. Weiss, Eloise Ferreira   +4 more
wiley   +1 more source

The dependence of silver staining of polyribonucleotides in polyacrylamide gels on nucleotide sequence [PDF]

, 1990
Описан простой и хорошо воспроизводимый метод окрашивания тРНК и гомополирибонуклеотидов серебром в денатурирующих ПААГ, чувствительность которого на три порядка превышает таковую при окрашивании этих полинуклеотидов метиленовым синим.
Назаренко, И.А., Мацкова, Л.В.   +1 more
core   +1 more source

ENZYMATIC-SYNTHESIS OF UNIFORMLY P-32-LABELED POLYRIBONUCLEOTIDES AND HIGH-SPECIFIC-ACTIVITY RIBONUCLEOSIDE 5'-[ALPHA-P-32]DIPHOSPHATES

, 1985
Uniformly 32P-labeled polyribonucleotides of high specific activity can be rapidly and easily synthesized from commercially available ribonucleoside 5-[alpha-32P]triphosphates by using two enzymes in sequence.
Kang, Changwon, Cantor, CR
core   +1 more source