Results 91 to 100 of about 23,515 (209)

Cloning of the Trichoderma reesei cDNA Encoding a Glucuronan Lyase Belonging to a Novel Polysaccharide Lyase Family [PDF]

Applied and Environmental Microbiology, 2009
ABSTRACT The filamentous fungus Trichoderma reesei produces glucuronan lyase (TrGL) when it is grown on β-(1→4)-polyglucuronate (cellouronate) as a sole carbon source. The cDNA encoding TrGL was cloned, and the recombinant enzyme was heterologously expressed in Pichia pastoris . The
Naotake, Konno, Kiyohiko, Igarashi, Naoto, Habu, Masahiro, Samejima, Akira, Isogai   +4 more
openaire   +2 more sources

Impact of different alginate lyases on combined cellulase–lyase saccharification of brown seaweed [PDF]

, 2016
Alginate attack characteristics and impact on cellulase–lyase catalyzed saccharification of brown seaweed were compared for three microbial PL7 alginate lyases (EC 4.2.2.-) two of them heterologously expressed in Escherichia coli as part of the work.
Manns, Dirk Martin, Meyer, Anne S., Nyffenegger, Christian, Saake, B.   +3 more
core   +1 more source

Characterisation of a phylogenetically distinct PL25 family ulvan lyase from a seaweed biomass enriched metagenome

The FEBS Journal, EarlyView.
Ulvan is a polysaccharide available from green seaweed with beneficial properties for various applications. The full potential of ulvan requires enzymatic degradation of the polymer, thus producing ulvan oligosaccharides. This study expands the armoury of characterised ulvan lyases.
Andrius Jasilionis, Pavithra Sivakumar, Justyna M. Dobruchowska, Sune Fjermedal, Hörður Guðmundsson, Bjorn Thor Adalsteinsson, Guðmundur Ó. Hreggviðsson, Anne S. Meyer, Eva Nordberg Karlsson   +8 more
wiley   +1 more source

Biochemical Characterization of a New Oligoalginate Lyase and Its Biotechnological Application in Laminaria japonica Degradation

Frontiers in Microbiology, 2020
Oligoalginate lyases catalyze the degradation of alginate polymers and oligomers into monomers, a prerequisite for biotechnological utilizing alginate.
Shangyong Li, Shangyong Li, Linna Wang, Samil Jung, Beom Suk Lee, Ningning He, Myeong-Sok Lee   +6 more
doaj   +1 more source

The binding of glycosaminoglycans to peptides : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry at Massey University [PDF]

, 1994
The overall aim this study was to examine the possibility of using immobilised polypeptide chains to fractionate/separate Glycosaminoglycans (GAG's) from mixtures. Initially individual samples of three GAG classes (chondroitin sulphate, dermatan sulphate
Taylor, Grant John
core  

The mucin-degradation strategy of Ruminococcus gnavus:The importance of intramolecular trans-sialidases [PDF]

, 2016
We previously identified and characterized an intramolecular trans-sialidase (IT-sialidase) in the gut symbiont Ruminococcus gnavus ATCC 29149, which is associated to the ability of the strain to grow on mucins. In this work we have obtained and analyzed
Crossman, Lisa C., Crost, Emmanuelle H, Henrissat, Bernard, Juge, Nathalie, Monestier, Marie, Swarbreck, David, Tailford, Louise E   +6 more
core   +2 more sources

Transcriptional adapter ADA2 regulates yield and end‐use quality through liquid–liquid phase separation in wheat

Journal of Integrative Plant Biology, EarlyView.
The transcriptional adapter ADA2 interacts with the histone acetyltransferase GCN5 to regulate starch and storage protein content in wheat grains through histone acetylation. ADA2 undergoes phase separation and is modulated by GCN5, affecting histone acetyltransferase activity and gene transcription.
Xiaobang Zhang, Ruijie Zhang, Yuhan Zhang, Tao Yang, Longfei Wu, Yuan Cao, Jun Xiao, Jigang Li, Xin Gao, Xinyou Cao, Jie Liu, Mingming Xin, Yuan Zong, Zhongfu Ni, Qixin Sun, Yingyin Yao   +15 more
wiley   +1 more source

Crystal structure of exo‐rhamnogalacturonan lyase from Penicillium chrysogenum as a member of polysaccharide lyase family 26 [PDF]

FEBS Letters, 2018
Exo‐rhamnogalacturonan lyase from Penicillium chrysogenum 31B (PcRGLX) was recently classified as a member of polysaccharide lyase (PL) family 26 along with hypothetical proteins derived from various organisms. In this study, we determined the crystal structure of PcRGLX as the first structure of a member of this family.
Yuika, Kunishige, Marin, Iwai, Masami, Nakazawa, Mitsuhiro, Ueda, Toshiji, Tada, Shigenori, Nishimura, Tatsuji, Sakamoto   +6 more
openaire   +2 more sources

Gallic Acid‐Responsive microRNAs Reprogram Lignification During Drought Acclimation Process in Spearmint

Plant Biotechnology Journal, EarlyView.
ABSTRACT Mentha spicata L. (spearmint) is a high‐value aromatic and medicinal species, whose productivity is strongly affected by water deficit. Nevertheless, the molecular mechanisms underlying drought acclimation in this mint remain largely unexplored. Thus, here, we investigated the microRNA‐mediated regulatory processes triggered in M.
Alessia D'Agostino, Gabriele Di Marco, Gerardo Pepe, Adelaide Teofani, Chiara Pontecorvi, Manuela Helmer‐Citterich, Antonella Canini, Angelo Gismondi   +7 more
wiley   +1 more source

Cloning and characterization of the first polysaccharide lyase family 6 oligoalginate lyase from marineShewanellasp. Kz7 [PDF]

Journal of Biochemistry, 2015
Alginate, the most abundant carbohydrate in brown macroalgae, is widely used in the food and pharmaceutical industries. Recently, alginate has attracted increasing attention, as it may serve as an alternative biomass for the production of biofuel. The degradation of alginate into monomeric units is the prerequisite for bioethanol production.
Shangyong, Li, Linna, Wang, Feng, Han, Qianhong, Gong, Wengong, Yu   +4 more
openaire   +2 more sources