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Polysaccharide Lyase: Molecular Cloning of Gellan Lyase Gene and Formation of the Lyase from a Huge Precursor Protein inBacillussp. GL1

Archives of Biochemistry and Biophysics, 1998
A bacterium, Bacillus sp. GL1, produced constitutively the extracellular polysaccharide-degrading enzyme (gellan lyase) with a molecular mass of 140 kDa. A genomic DNA library of the bacterium was constructed in Escherichia coli using the cosmid vector, Charomid 9-36.
W, Hashimoto   +3 more
openaire   +2 more sources

Ferritin-tagged ulva polysaccharide lyase for efficient degradation of biomass polysaccharides into reducing sugars

Bioprocess and Biosystems Engineering
Enhancing the stability and the reusability of ulva polysaccharide lyase (UPL) is crucial for the efficient production of reducing sugars from ulva polysaccharides, which are vital for their broad applications in functional foods. In this study, we innovatively developed a self-immobilized UPL by fusing the enzyme with ferritin, leading to the ...
Qing, Yang   +11 more
openaire   +2 more sources

Preparation of branched hexasaccharides by the action of a viral lyase on klebsiella K14 polysaccharide

Carbohydrate Research, 1988
Klebsiella K14 capsular polysaccharide was degraded by a bacteriophage-borne enzyme to afford oligosaccharides A-C which were studied by one- and two-dimensional n.m.r. spectroscopy. A and B were the repeating-unit hexasaccharide and pyruvylated hexasaccharide, respectively, while C was a dodecasaccharide.
H, Parolis, L A, Parolis, G G, Dutton
openaire   +2 more sources

Influence of the culture conditions on extracellular lyase activity related to K5 polysaccharide

Biotechnology Letters, 2000
The K5 capsular polysaccharide antigen of some Escherichia coli strains is the non-sulphated precursor in heparin biosynthesis. It is composed by two components, 16 000 and 1500 Da, whose ratio depends on the activity of the extracellular form of a lyase synthesized by the same K5 producer strain.
M. Manzoni, S. Bergomi, M. Rollini
openaire   +1 more source

Two Alginate Lyases from Polysaccharide Lyase Family 44 Exhibited Diversity in Substrate Specificity

Journal of Agricultural and Food Chemistry
Alginate lyases are important tools for alginate biodegradation and oligosaccharide preparation. This study characterized the two alginate lyases, Aly44An and Aly44Pa, from the newly constructed polysaccharide lyase family 44 (PL44). Aly44An exhibited the highest enzyme activity and conversion efficiency for polyM and also showed activity for alginate ...
Jiajing Li   +6 more
openaire   +2 more sources

Mechanistic insights into catalysis of a novel polysaccharide lyase family 40 ulvan lyase from Thalassomonas sp. LD5

International Journal of Biological Macromolecules
As one of the primary structural components in green algal cell walls, ulvan can be enzymatically degraded by ulvan lyase to produce oligosaccharides with diverse biological activities. In this study, TsUly40A, a new PL40 family ulvan lyase from Thalassomonas sp.
Zheng, Fu   +9 more
openaire   +2 more sources

Polyuronic acid degradation by polysaccharide lyase family 7

Acta Crystallographica Section A Foundations and Advances, 2022
M. Vuillemin   +12 more
openaire   +1 more source

Characterization of an organic solvent-tolerant polysaccharide lyase from Microbulbifer thermotolerans DAU221

International Journal of Biological Macromolecules, 2021
Yu-Sin Jang, Yong-Lark Choi
exaly  

Polysaccharide Lyases

2017
S. Chakraborty   +3 more
openaire   +1 more source

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