Results 141 to 150 of about 7,014 (162)

In situ hybridization and immunohistochemistry for the detection of porcine cytomegalovirus

Journal of Virological Methods, 2012
To establish in situ hybridization and immunohistochemistry based-assays for the detection of porcine cytomegalovirus, routinely processed renal tissue sections from 34 diseased piglets suspected of having the infection were obtained and examined. Using hematoxylin and eosin, porcine cytomegalovirus inclusion bodies were found in the nucleus of renal ...
Tomoyuki Shibahara, Ayako Miyazaki, Tomoko Tajima   +2 more
exaly   +3 more sources

Isolation and Experimental Infection with Porcine Cytomegalovirus

Zentralblatt für Veterinärmedizin Reihe B, 1977
SummaryFive strains of porcine cytomegalovirus (PCMV) were isolated in cultures of pig lung macrophages (PLM) from pigs aged about 3 weeks. Two of them were isolated from the nasal mucosa and the remaining three from the lung. The presence of PCMV in PLM cultures was demonstrated indirectly by staining and directly by electron microscopy.
L, Valícek, B, Smíd, J, Mensík
openaire   +2 more sources

Porcine cytomegalovirus (PCMV) in early gestation

Veterinary Microbiology, 1988
Following intranasal exposure to PCMV at or within 48 h of coitus transplacental infection occurred in two groups of gilts. Five out of 22 embryos were infected in the first group but only 2 out of 63 in the second. A more rapid immune response as measured by circulating antibody was probably instrumental in abrogating infection in the second group. In
N, Edington, A E, Wrathall, J T, Done
openaire   +2 more sources

Superinfection with porcine cytomegalovirus initiating transplacental infection

Veterinary Microbiology, 1988
Four sows with circulating antibody were exposed to porcine cytomegalovirus. Virus was detected in 8 of 24 foetuses by immunofluorescence and/or virus isolation from 2 sows with low levels of antibody. In 6 of the infected foetuses, the virus was in capillary endothelium and macrophages of the lung, and was associated with interlobular oedema in 2 of ...
N, Edington, S, Broad, A E, Wrathall, J T, Done   +3 more
openaire   +2 more sources

Porcine Cytomegalovirus/Porcine Roseolovirus (PCMV/PRV) and Antiviral Drugs

Xenotransplantation
Joachim Denner
exaly   +3 more sources

A new Western blot assay for the detection of porcine cytomegalovirus (PCMV)

Journal of Immunological Methods, 2016
Porcine cytomegalovirus (PCMV) may be harmful for human recipients if xenotransplantation using pig cell, tissue or organ will be performed transmitting the virus from donor pigs to human recipients. PCMV is widespread in pigs and closely related to human pathogenic herpesviruses, however there are no data concerning infection of humans.
Joachim Denner
exaly   +3 more sources

Human Fibroblasts Are Permissive for Porcine Cytomegalovirus In Vitro

Transplantation, 2008
Xenotransplantation with pig organs is being considered to alleviate donor organ shortages; however, the risk of introducing porcine viruses into humans is heightened in this setting. The goal of this study was to determine the infectious potential of porcine cytomegalovirus (PCMV), a xenozoonotic virus of interest, in human fibroblasts in vitro ...
Jennifer L, Whitteker, Anil K, Dudani, Eilleen S, Tackaberry   +2 more
openaire   +2 more sources

Porcine Cytomegalovirus (PCMV)

1989
Cytomegalovirus infections are very common in many species (1,2,3,4,5). They induce host specific cytomegalic intranuclear inclusions in various organs. PCMV was first identified as inclusion body rhinitis (IBR) of pigs in Great Britain by Done and coworkers in 1955 (6). Obviously PCMV is inapparently present in most pig herds and causes generally mild
openaire   +1 more source

Electron Microscopy of Porcine Cytomegalovirus in Pig Lung Macrophage Cultures

Zentralblatt für Veterinärmedizin Reihe B, 2010
Summary Two strains of porcine cytomegalovirus (PCMV), strain B 4 and the reference strain ADRI-1, were studied in culture of pig lung macrophages (PLM) by electron microscopy. The nuclei of PLM contained various forms of nucleocapsids of differing core structure.
L, Valícek, B, Smíd
openaire   +2 more sources

Identification and Sequence Analysis of the Glycoprotein B Gene of Porcine Cytomegalovirus

Virus Genes, 2001
Porcine cytomegalovirus (PCMV) is one of the pathogens that should be eliminated from pigs intended for use as organ donors in xenotransplantation. For this purpose, reliable diagnostic test systems are needed. To provide a basis for this goal and to analyse the evolutionary relationships of PCMV within the herpesvirus family, the putative glycoprotein
F, Widen, M, Goltz, N, Wittenbrink, B, Ehlers, M, Banks, S, Belak   +5 more
openaire   +2 more sources