Results 131 to 140 of about 774,815 (306)

HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein

FEBS Open Bio, EarlyView.
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley   +1 more source

BMI‐1 modulation and trafficking during M phase in diffuse intrinsic pontine glioma

FEBS Open Bio, EarlyView.
The schematic illustrates BMI‐1 phosphorylation during M phase, which triggers its translocation from the nucleus to the cytoplasm. In cycling cells, BMI‐1 functions within the PRC1 complex to mediate H2A K119 monoubiquitination. Following PTC596‐induced M phase arrest, phosphorylated BMI‐1 dissociates from PRC1 and is exported to the cytoplasm via its
Banlanjo Umaru, Deepak Kumar Mishra, Shiva Senthil Kumar, Hao‐Han Pang, Brendan Devine, Komal Khan, Rachid Drissi   +6 more
wiley   +1 more source

Fine-tuning thermogenesis from the post-transcriptional level

iScience
Summary: In mammals, adipose tissue is involved in energy homeostasis regulation. White adipose tissue (WAT) is the major energy storage site for mammals, whereas brown adipose tissue (BAT) is involved in energy expenditure and thermogenesis.
Mengyao Wan, Hao Gu, Siyuan Chen, Zihao Guo, Bayindala Xiagedeer, Yaqun Guan, Xiaodi Liang   +6 more
doaj   +1 more source

The cooperative regulation of miR‐221 by APE1 and AUF1 impacts p27Kip1 defining a miR signature relevant for cervical cancer

FEBS Open Bio, EarlyView.
A regulatory axis involving APE1, AUF1, and miR‐221 is proposed. Pri‐miR‐221 is processed by DROSHA and DICER to generate mature miR‐221, which targets p27Kip1 mRNA. APE1 and AUF1 compete for pre‐miR‐221 binding. Reduced APE1/AUF1 levels impair miR‐221 biogenesis, decrease p27Kip1 mRNA degradation, and promote cell cycle progression, chemoresistance ...
Matilde Clarissa Malfatti, Nicolò Gualandi, Gianluca Tell, Giulia Antoniali   +3 more
wiley   +1 more source

Meta‐analysis fails to show any correlation between protein abundance and ubiquitination changes

FEBS Open Bio, EarlyView.
We analyzed over 50 published proteomics datasets to explore the relationship between protein levels and ubiquitination changes across multiple experimental conditions and biological systems. Although ubiquitination is often associated with protein degradation, our analysis shows that changes in ubiquitination do not globally correlate with changes in ...
Nerea Osinalde, Unai Alduntzin, Gorka Prieto, Ugo Mayor   +3 more
wiley   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Molecular mechanisms involved in Plasmodium gametocytogenesis

Frontiers in Microbiology
Malaria, a disease caused by protozoa of the genus Plasmodium, remains a major challenge for global public health. The persistence of disease transmission to the mosquito vector depends on the differentiation of asexual blood-stage parasites into ...
Aline Miranda Scovino, Rafaella Stéfany Oliveira-da-Silva, Rafaella Stéfany Oliveira-da-Silva, Joyce Almeida-da-Silva, Joyce Almeida-da-Silva, Karolynne Dantas Mendes, Elias Barbosa da Silva-Junior, Debora Decote-Ricardo, Leonardo Freire-de-Lima, Celio Geraldo Freire-de-Lima, Paulo Renato Rivas Totino, Alexandre Morrot, Alexandre Morrot   +12 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

A Reference‐Free Algorithm Discovers Regulation in the Plant Transcriptome

Plant Direct
Most plant genomes and their (post‐)transcriptional regulation remain unknown. We used SPLASH—a new, reference genome‐free sequence variation detection algorithm—to analyze transcriptional and post‐transcriptional regulation from RNA‐seq data.
Elisabeth Meyer, Evan V. Saldivar, Marek Kokot, Bo Xue, Sebastian Deorowicz, Seung Y. Rhee, Julia Salzman   +6 more
doaj   +1 more source

Interplay between single-stranded binding proteins on RNA secondary structure

, 2013
RNA protein interactions control the fate of cellular RNAs and play an important role in gene regulation. An interdependency between such interactions allows for the implementation of logic functions in gene regulation.
Bundschuh, Ralf, Lin, Yi-Hsuan
core   +1 more source