Results 101 to 110 of about 14,528 (201)

How the initiating ribosome copes with ppGpp to translate mRNAs.

PLoS Biology, 2020
During host colonization, bacteria use the alarmones (p)ppGpp to reshape their proteome by acting pleiotropically on DNA, RNA, and protein synthesis. Here, we elucidate how the initiating ribosome senses the cellular pool of guanosine nucleotides and ...
Daria S Vinogradova, Victor Zegarra, Elena Maksimova, Jose Alberto Nakamoto, Pavel Kasatsky, Alena Paleskava, Andrey L Konevega, Pohl Milón   +7 more
doaj   +1 more source

Contributions of SpoT Hydrolase, SpoT Synthetase, and RelA Synthetase to Carbon Source Diauxic Growth Transitions in Escherichia coli

Frontiers in Microbiology, 2018
During the diauxic shift, Escherichia coli exhausts glucose and adjusts its expression pattern to grow on a secondary carbon source. Transcriptional profiling studies of glucose–lactose diauxic transitions reveal a key role for ppGpp. The amount of ppGpp
Llorenç Fernández-Coll, Michael Cashel
doaj   +1 more source

Robotic‐Assisted Capture‐Systematic Evolution of Ligands by Exponential Enrichment of RNA Aptamers Binding to Small Molecules

ChemBioChem, Volume 26, Issue 15, August 22, 2025.
This study demonstrates the rapid selection of RNA aptamers that can target various small molecules through a newly established automated capture‐systematic evolution of ligands by exponential enrichment technique. Throughout the study, this method is applied to aptamer selection for several small molecules, resulting in aptamers that bind with high ...
Tjasa Legen, Günter Mayer
wiley   +1 more source

Essential Roles for Mycobacterium tuberculosis Rel beyond the Production of (p)ppGpp [PDF]

, 2013
In Mycobacterium tuberculosis, the stringent response to amino acid starvation is mediated by the M. tuberculosis Rel (Rel(Mtb)) enzyme, which transfers a pyrophosphate from ATP to GDP or GTP to synthesize ppGpp and pppGpp, respectively.
Stallings, Christina L., Weiss, Leslie A.   +1 more
core   +2 more sources

Macrophage‐derived reactive oxygen species promote Salmonella aggresome formation contributing to bacterial antibiotic persistence

iMeta, Volume 4, Issue 4, August 2025.
In this study, we reveal that macrophage‐derived reactive oxygen species (ROS) can trigger the rapid formation of Salmonella aggresomes, which substantially contribute to the increased frequency of persisters induced by phagocytosis. Salmonella containing aggresomes exhibited a dormant phenotype characterized by reduced adenosine triphosphate (ATP ...
Xiao Chen, Kefan Fang, Bo Li, Yingxing Li, Yuehua Ke, Weixin Ke, Tian Tian, Yifan Zhao, Linqi Wang, Jing Geng, Mark C. Leake, Fan Bai   +11 more
wiley   +1 more source

Comparative proteomic analysis of Salmonella enterica serovar Typhimurium ppGpp-deficient mutant to identify a novel virulence protein required for intracellular survival in macrophages

BMC Microbiology, 2010
Background The global ppGpp-mediated stringent response in pathogenic bacteria plays an important role in the pathogenesis of bacterial infections. In Salmonella enterica serovar Typhimurium (S. Typhimurium), several genes, including virulence genes, are
Kumagai Yoshinori, Miki Tsuyoshi, Shimizu-Izumi Satomi, Maeda Tadakazu, Oh-Ishi Masamichi, Kodera Yoshio, Yoshida-Ohta Yukie, Sugimoto Mariko, Haneda Takeshi, Danbara Hirofumi, Okada Nobuhiko   +10 more
doaj   +1 more source

Pb2+ tolerance by Frankia sp. strain EAN1pec involves surface-binding [PDF]

, 2017
Several Frankia strains have been shown to be lead-resistant. The mechanism of lead resistance was investigated for Frankia sp. strain EAN1pec. Analysis of the cultures by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDAX ...
Furnholm, Teal, Rehan, Medhat, Tisa, Louis S., Wishart, Jessica   +3 more
core   +2 more sources

Putative Transcriptional Regulator LysR1 Is Required for Full Virulence of Erwinia amylovora

Plant Pathology, Volume 74, Issue 6, Page 1697-1706, August 2025.
Erwinia amylovora lysR1 transcription factor mutants have a sharply reduced ability to cause fire blight disease, not due to lysine auxotrophy but a deficiency in production of the virulence factor exopolysaccharide amylovoran. ABSTRACT Fire blight, caused by the gram‐negative bacterium Erwinia amylovora, is a serious disease of apple and pear trees ...
Sara M. Klee, Judith P. Sinn, Jeremy B. Held, Francesco Pacini, Aleah C. Holmes, Melissa Finley, Brian L. Lehman, Kari A. Peter, Timothy W. McNellis   +8 more
wiley   +1 more source

Polyphosphate and associated enzymes as global regulators of stress response and virulence in Campylobacter jejuni [PDF]

, 2016
Campylobacter jejuni (C. jejuni), a Gram-negative microaerophilic bacterium, is a predominant cause of bacterial foodborne gastroenteritis in humans worldwide.
Gangaiah, Dharanesh, Kumar, Anand, Rajashekara, Gireesh, Torrelles, Jordi B.   +3 more
core   +1 more source

Comparative study of adenosine 3′‐pyrophosphokinase domains of MuF polymorphic toxins

FEBS Open Bio, Volume 15, Issue 7, Page 1103-1112, July 2025.
With the ultimate goal of understanding the association of toxin‐immunity modules to temperate phages, we characterized toxins from three prophages and examined cross‐protection from immunity proteins. The toxins exhibit adenosine 3′‐pyrophosphokinase activity and are toxic in Escherichia coli.
Eloïse M. Paulet, Julia Bartoli, Atakan Kabtan, Chloé M. Piras, Audrey C. Tempier, Eric Cascales, Julie P. Viala   +6 more
wiley   +1 more source