Results 61 to 70 of about 7,972 (181)

Transcription of photosynthetic genes in the plant chloroplast: lessons from bacteria

The Plant Journal, Volume 126, Issue 5, June 2026.
SUMMARY The RNA polymerase that transcribes photosynthetic genes in the plant chloroplast is the largest known transcription enzyme across all domains of life, comprising 21 subunits of bacterial and eukaryotic origin. Recent structural analyses revealed that the core polymerase, inherited from the cyanobacterial ancestor of the chloroplast, is encased
Jessica Moon, Ashley Dudey, Ángel Vergara‐Cruces, William Tasker‐Brown, David Pearce, Michael W. Webster   +5 more
wiley   +1 more source

The Absence of (p)ppGpp Renders Initiation of Escherichia coli Chromosomal DNA Synthesis Independent of Growth Rates

mBio, 2020
The initiation of Escherichia coli chromosomal DNA replication starts with the oligomerization of the DnaA protein at repeat sequences within the origin (ori) region. The amount of ori DNA per cell directly correlates with the growth rate.
Llorenç Fernández-Coll, Monika Maciag-Dorszynska, Krishma Tailor, Stephen Vadia, Petra Anne Levin, Agnieszka Szalewska-Palasz, Michael Cashel   +6 more
doaj   +1 more source

Structural Basis for Transcription Regulation by Alarmone ppGpp [PDF]

Cell, 2004
Guanosine-tetraphosphate (ppGpp) is a major regulator of stringent control, an adaptive response of bacteria to amino acid starvation. The 2.7 A resolution structure of the Thermus thermophilus RNA polymerase (RNAP) holoenzyme in complex with ppGpp reveals that ppGpp binds to the same site near the active center in both independent RNAP molecules in ...
Artsimovitch, Irina, Patlan, Vsevolod, Sekine, Shun-ichi, Vassylyeva, Marina N, Hosaka, Takeshi, Ochi, Kozo, Yokoyama, Shigeyuki, Vassylyev, Dmitry G   +7 more
openaire   +2 more sources

Viable but Nonculturable State, a Survival Strategy for Salmonella in Aquatic Environments

Water and Environment Journal, Volume 40, Issue 2, Page 183-202, May 2026.
Transition of Salmonella from a culturable state to a viable but nonculturable state under various environmental stresses and current methods for detecting viable Salmonella in environmental samples. ABSTRACT In the relentless battle for survival, Salmonella has evolved mechanisms to withstand harsh conditions such as extreme temperatures, fluctuating ...
Sanelisiwe Thinasonke Duze, Musa Marimani, Katlego Mamabolo, Mrudula Patel   +3 more
wiley   +1 more source

Broad-spectrum anti-biofilm peptide that targets a cellular stress response. [PDF]

PLoS Pathogens, 2014
Bacteria form multicellular communities known as biofilms that cause two thirds of all infections and demonstrate a 10 to 1000 fold increase in adaptive resistance to conventional antibiotics.
César de la Fuente-Núñez, Fany Reffuveille, Evan F Haney, Suzana K Straus, Robert E W Hancock   +4 more
doaj   +1 more source

Kontrollierter Transport und lichtinduzierte Freisetzung von Magic Spot Nukleotiden in Escherichia coli

Angewandte Chemie, Volume 138, Issue 15, 6 April 2026.
We synthesized photocaged, clickable and isotope‐labeled “magic spot” Nucleotides and delivered them into Escherichia coli comparing different approaches. Light‐controlled release inside living cells enabled tracking of their conversion from pppGpp to ppGpp by capillary electrophoresis mass spectrometry and revealed their impact on the growth rate of a
Christoph Popp, Patrick Moser, Anselm Schwoerbel, Xinwei Liu, Johannes Freitag, Pinku Sarmah, Isabel Prucker, Robert Zscherp, Xuan Wang, Philipp Klahn, Hans‐Georg Koch, Gert Bange, Henning J. Jessen   +12 more
wiley   +1 more source

σ54-Promoter Discrimination and Regulation by ppGpp and DksA [PDF]

Journal of Biological Chemistry, 2009
The sigma(54)-factor controls expression of a variety of genes in response to environmental cues. Much previous work has implicated the nucleotide alarmone ppGpp and its co-factor DksA in control of sigma(54)-dependent transcription in the gut commensal Escherichia coli, which has evolved to live under very different environmental conditions than ...
Lisandro M D, Bernardo, Linda U M, Johansson, Eleonore, Skärfstad, Victoria, Shingler   +3 more
openaire   +2 more sources

The Use of DeepQSAR Models for the Discovery of Peptides With Enhanced Antimicrobial and Antibiofilm Potential

Molecular Informatics, Volume 45, Issue 4, April 2026.
A DeepQSAR framework integrating a feed‐forward neural network and a recurrent neural network (RNN) was developed to identify antimicrobial peptides with enhanced antibiofilm and antimicrobial activity. The feed‐forward model was trained on in‐house antibiofilm IC50 data to capture quantitative structure–activity relationships, and its learned features
Jiaying You, Hazem Mslati, Evan F. Haney, Noushin Akhoundsadegh, Robert E. W. Hancock, Artem Cherkasov   +5 more
wiley   +1 more source

Quinolone tolerance in Escherichia coli due to defects in the adenosine ribonucleotides de novo biosynthesis pathway

mLife, Volume 5, Issue 2, Page 217-228, April 2026.
Abstract Central carbon metabolism is thought to link reactive oxygen species (ROS) with antibiotic‐mediated bacterial death. During enrichment screening of Escherichia coli with the first‐generation quinolone oxolinic acid, unstable antibiotic‐tolerant mutants containing deficiencies in purB were obtained.
Weiwei Zhu, Yuejuan Nong, Jie Su, Jingwen Yang, Lina Ma, Yunxin Xue, Dai Wang, Jianjun Niu, Karl Drlica, Xilin Zhao   +9 more
wiley   +1 more source

Contributions of SpoT Hydrolase, SpoT Synthetase, and RelA Synthetase to Carbon Source Diauxic Growth Transitions in Escherichia coli

Frontiers in Microbiology, 2018
During the diauxic shift, Escherichia coli exhausts glucose and adjusts its expression pattern to grow on a secondary carbon source. Transcriptional profiling studies of glucose–lactose diauxic transitions reveal a key role for ppGpp. The amount of ppGpp
Llorenç Fernández-Coll, Michael Cashel
doaj   +1 more source