Results 31 to 40 of about 211,616 (238)

Retroelement origins of pre‐mRNA splicing [PDF]

WIREs RNA, 2020
AbstractRecent cryo‐EM structures of a group II intron caught in the process of invading DNA have given new insight into the mechanisms of both splicing and retrotransposition. Conformational dynamics involving the branch‐site helix domain VI are responsible for substrate exchange between the two steps of splicing.
Daniel B. Haack, Navtej Toor
openaire   +2 more sources

H3K36 Methylation and the Chromodomain Protein Eaf3 Are Required for Proper Cotranscriptional Spliceosome Assembly

Cell Reports, 2019
Summary: In the eukaryotic cell, spliceosomes assemble onto pre-mRNA cotranscriptionally. Spliceosome assembly takes place in the context of the chromatin environment, suggesting that the state of the chromatin may affect splicing.
Calvin S. Leung, Stephen M. Douglass, Marco Morselli, Matthew B. Obusan, Marat S. Pavlyukov, Matteo Pellegrini, Tracy L. Johnson   +6 more
doaj   +1 more source

Interaction of yeast eIF4G with spliceosome components Implications in pre-mRNA processing events [PDF]

, 2009
International audienceAs evidenced from mammalian cells the eukaryotic translation initiation factor eIF4G has a putative role in nuclear RNA metabolism. Here we investigate whether this role is conserved in the yeast Saccharomyces cerevisiae.
Barrass, J. David, Beggs, Jean D., Fromont-Racine, Micheline, Jacquier, Alain, Kafasla, Panagiota, Lewis, Joe, Thompson, Elizabeth   +6 more
core   +2 more sources

Interconnections Between RNA-Processing Pathways Revealed by a Sequencing-Based Genetic Screen for Pre-mRNA Splicing Mutants in Fission Yeast

G3: Genes, Genomes, Genetics, 2016
Pre-mRNA splicing is an essential component of eukaryotic gene expression and is highly conserved from unicellular yeasts to humans. Here, we present the development and implementation of a sequencing-based reverse genetic screen designed to identify ...
Amy Larson, Benjamin Jung Fair, Jeffrey A. Pleiss   +2 more
doaj   +1 more source

Interaction of eukaryotic translation initiation factor 4G with the nuclear cap-binding complex provides a link between nuclear and cytoplasmic functions of the m7 guanosine cap [PDF]

, 2001
In eukaryotes the majority of mRNAs have an m7G cap that is added cotranscriptionally and that plays an important role in many aspects of mRNA metabolism.
Afonina E., Boisvert F. M., Bushell M., Carmo-Fonseca M., Cho E. J., Dostie J., Dostie J., Ferreira J. A., Flaherty S. M., Fornerod M., Fortes P., Fortes P., Fraser C. S., Fromont-Racine M., Fu X. D., Gingras A. C., Gorlich D., Hentze M., Ho C. K., Izaurralde E., Izaurralde E., Jarmolowski A., Kataoka N., Kim C. Y., Kudo N., Le Hir H., Le Hir H., Lewis J. D., Lewis J. D., Lewis J. D., Lewis J. D., Martins L. M., McCracken S., Milligan J. F., Morley S. J., Morley S. J., Neugebauer K. M., Neugebauer K. M., Ohno M., Pettersson I., Piron M., Ponting C. P., Preiss T., Rozen F., Salditt-Georgieff M., Schaegger H., Shatkin A. J., Stade K., Visa N., Watanabe M., Zhou Z.   +50 more
core   +2 more sources

Epigenetics in Alternative Pre-mRNA Splicing [PDF]

Cell, 2011
Alternative splicing plays critical roles in differentiation, development, and disease and is a major source for protein diversity in higher eukaryotes. Analysis of alternative splicing regulation has traditionally focused on RNA sequence elements and their associated splicing factors, but recent provocative studies point to a key function of chromatin
Luco, Reini F., Alló, Mariano, Schor, Ignacio Esteban, Kornblihtt, Alberto Rodolfo, Misteli, Tom   +4 more
openaire   +3 more sources

Splicing-dependent NMD does not require the EJC in Schizosaccharomyces pombe [PDF]

, 2010
Nonsense-mediated mRNA decay (NMD) is a translation-linked process that destroys mRNAs with premature translation termination codons (PTCs). In mammalian cells, NMD is also linked to pre-mRNA splicing, usually PTCs trigger strong NMD only when positioned
Carter MS, Culbertson MR, Hodgkin J, Jikai Wen, Le Hir H, Muhlemann O, Peltz SW, Saverio Brogna, Zhang J, Zhang SA   +9 more
core   +3 more sources

Alterations of Alternative Splicing Patterns of Ser/Arg-Rich (SR) Genes in Response to Hormones and Stresses Treatments in Different Ecotypes of Rice (Oryza sativa)

Journal of Integrative Agriculture, 2013
Ser/Arg-rich (SR) genes encode proteins that play pivotal roles in both constitutive and alternative splicing of pre-mRNA. However, not much effort has been made to investigate the alternative splicing of their own pre-mRNA.
Peng ZHANG, Heng DENG, Fang-ming XIAO, Yong-sheng LIU   +3 more
doaj   +1 more source

Modulation of PKM alternative splicing by PTBP1 promotes gemcitabine resistance in pancreatic cancer cells [PDF]

, 2015
Pancreatic ductal adenocarcinoma (PDAC) is an aggressive and incurable disease. Poor prognosis is due to multiple reasons, including acquisition of resistance to gemcitabine, the first-line chemotherapeutic approach.
Bielli, P, Calabretta, S, Capurso, G, DELLE FAVE, Gianfranco, Fendrich, V, Passacantilli, I, Pilozzi, Emanuela, Sette, C.   +7 more
core   +2 more sources

Pre-mRNA splicing and human disease [PDF]

Genes & Development, 2003
The precision and complexity of intron removal during pre-mRNA splicing still amazes even 26 years after the discovery that the coding information of metazoan genes is interrupted by introns (Berget et al. 1977; Chow et al. 1977). Adding to this amazement is the recent realization that most human genes express more than one mRNA by alternative splicing,
Nuno André, Faustino, Thomas A, Cooper
openaire   +2 more sources