In Vitro Studies of the Prp9·Prp11·Prp21 Complex Indicate a Pathway for U2 Small Nuclear Ribonucleoprotein Activation [PDF]
, 1996 Pre-mRNA splicing takes place on a large ribonucleoprotein particle, the spliceosome which contains the five small nuclear ribonucleoproteins (snRNPs), U1, U2, U4, U5, and U6. In Saccharomyces cerevisiae the mRNA splicing factors, Prp9, Prp11, and Prp21,
Abelson, John +2 more
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Structural Insights into Nuclear pre-mRNA Splicing in Higher Eukaryotes.
Cold Spring Harbor Perspectives in Biology, 2019 SUMMARYThe spliceosome is a highly complex, dynamic ribonucleoprotein molecular machine that undergoes numerous structural and compositional rearrangements that lead to the formation of its active site. Recent advances in cyroelectron microscopy (cryo-EM)
B. Kastner +3 more
semanticscholar +1 more source
Retroelement origins of pre‐mRNA splicing [PDF]
WIREs RNA, 2020 AbstractRecent cryo‐EM structures of a group II intron caught in the process of invading DNA have given new insight into the mechanisms of both splicing and retrotransposition. Conformational dynamics involving the branch‐site helix domain VI are responsible for substrate exchange between the two steps of splicing.
Daniel B. Haack, Navtej Toor
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A quantitative, high-throughput reverse genetic screen reveals novel connections between Pre-mRNA splicing and 5' and 3' end transcript determinants. [PDF]
PLoS Genetics, 2012 Here we present the development and implementation of a genome-wide reverse genetic screen in the budding yeast, Saccharomyces cerevisiae, that couples high-throughput strain growth, robotic RNA isolation and cDNA synthesis, and quantitative PCR to allow
Laura-Oana Albulescu +6 more
doaj +1 more source
Regulation of alternative pre-mRNA splicing [PDF]
Briefings in Functional Genomics and Proteomics, 2002 The first wave of bioinformatic studies that followed genome and complementary DNA sequencing projects revealed that alternative splicing of messenger RNA precursors (pre-mRNAs) contributes substantially to transcriptome complexity in higher eukaryotes.
Juan Valcárcel, Louise Woodley
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eLife, 2022 Splicing is highly regulated and is modulated by numerous factors. Quantitative predictions for how a mutation will affect precursor mRNA (pre-mRNA) structure and downstream function are particularly challenging.
Jayashree Kumar +7 more
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General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer [PDF]
, 1993 The general splicing factor SF2/ASF binds in a sequence-specific manner to a purine-rich exonic splicing enhancer (ESE) in the last exon of bovine growth hormone (bGH) pre-mRNA.
Hampson, R. K. +4 more
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Molecular Mechanisms of pre-mRNA Splicing through Structural Biology of the Spliceosome.
Cold Spring Harbor Perspectives in Biology, 2019 SUMMARYPrecursor messenger RNA (pre-mRNA) splicing is executed by the spliceosome. In the past 3 years, cryoelectron microscopy (cryo-EM) structures have been elucidated for a majority of the yeast spliceosomal complexes and for a few human spliceosomes.
Chuangye Yan, R. Wan, Yigong Shi
semanticscholar +1 more source
Characterization of a disease-associated mutation affecting a putative splicing regulatory element in intron 6b of the cystic fibrosis transmembrane conductance regulator (CFTR) gene [PDF]
, 2009 Cystic fibrosis (CF) is a common recessive disorder caused by >1600 mutations in the CF transmembrane conductance regulator (CFTR) gene. About 13% of CFTR mutations are classified as “splicing mutations,” but for almost 40% of these, their role in ...
BAFFICO AM +8 more
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Genome Research, 2019 Pre-mRNA-splicing and adenosine to inosine (A-to-I) RNA-editing occur mostly cotranscriptionally. During A-to-I editing, a genomically encoded adenosine is deaminated to inosine by adenosine deaminases acting on RNA (ADARs).
Konstantin Licht +6 more
semanticscholar +1 more source