Results 21 to 30 of about 798,140 (253)

iCLIP: Protein–RNA interactions at nucleotide resolution [PDF]

Methods, 2014
RNA-binding proteins (RBPs) are key players in the post-transcriptional regulation of gene expression. Precise knowledge about their binding sites is therefore critical to unravel their molecular function and to understand their role in development and disease.
Huppertz, Ina, Attig, Jan, D’Ambrogio, Andrea, Easton, Laura E., Sibley, Christopher R., Sugimoto, Yoichiro, Tajnik, Mojca, König, Julian, Ule, Jernej   +8 more
openaire   +5 more sources

A translational repression reporter assay for the analysis of RNA-binding protein consensus sites

RNA Biology, 2023
RNA-binding proteins are essential regulators of RNA processing and function. Translational repression assays can be used to study how they interact with specific RNA sequences by insertion of such a consensus sequence into the 5’ untranslated region of ...
Jessica Nowacki, Mateo Malenica, Stefan Schmeing, Damian Schiller, Benjamin Buchmuller, Gulshan Amrahova, Peter ‘t Hart   +6 more
doaj   +1 more source

Transcriptome-Wide Identification of Coding and Noncoding RNA-Binding Proteins Defines the Comprehensive RNA Interactome of Leishmania mexicana

Microbiology Spectrum, 2022
Proteomic profiling of RNA-binding proteins in Leishmania is currently limited to polyadenylated mRNA-binding proteins, leaving proteins that interact with nonadenylated RNAs, including noncoding RNAs and pre-mRNAs, unidentified.
Karunakaran Kalesh, Wenbin Wei, Brian S. Mantilla, Theodoros I. Roumeliotis, Jyoti Choudhary, Paul W. Denny   +5 more
doaj   +1 more source

Affinity chromatography using 2′ fluoro-substituted RNAs for detection of RNA-protein interactions in RNase-rich or RNase-treated extracts

BioTechniques, 2009
Use of RNA affinity chromatography is commonly used to identify RNA binding proteins that interact with specific RNA cis-elements that function in post-transcriptional gene regulation.
Ruben H. Hovhannisyan, Russ P. Carstens
doaj   +1 more source

Multivalent interactions with RNA drive recruitment and dynamics in biomolecular condensates in Xenopus oocytes

iScience, 2022
Summary: RNA localization and biomolecular condensate formation are key biological strategies for organizing the cytoplasm and generating cellular polarity. In Xenopus oocytes, RNAs required for germ layer patterning localize in biomolecular condensates,
Sarah E. Cabral, Jessica P. Otis, Kimberly L. Mowry   +2 more
doaj   +1 more source

Identification of proteins binding coding and non-coding human RNAs using protein microarrays

BMC Genomics, 2012
Background The regulation and function of mammalian RNAs has been increasingly appreciated to operate via RNA-protein interactions. With the recent discovery of thousands of novel human RNA molecules by high-throughput RNA sequencing, efficient methods ...
Siprashvili Zurab, Webster Dan E, Kretz Markus, Johnston Danielle, Rinn John L, Chang Howard Y, Khavari Paul A   +6 more
doaj   +1 more source

Compendium of Methods to Uncover RNA-Protein Interactions In Vivo

Methods and Protocols, 2021
Control of gene expression is critical in shaping the pro-and eukaryotic organisms’ genotype and phenotype. The gene expression regulatory pathways solely rely on protein–protein and protein–nucleic acid interactions, which determine the fate of the ...
Mrinmoyee Majumder, Viswanathan Palanisamy   +1 more
doaj   +1 more source

Probing Long Non-coding RNA-Protein Interactions

Frontiers in Molecular Biosciences, 2017
Non-coding RNA sequences outnumber the protein-coding genes in the human genome, however our knowledge of their functions is still limited. RNA-binding proteins follow the transcripts, including non-coding RNAs, throughout their life, regulating not only
Jasmine Barra, Jasmine Barra, Eleonora Leucci   +2 more
doaj   +1 more source

The Nuclear Export Receptors TbMex67 and TbMtr2 Are Required for Ribosome Biogenesis in Trypanosoma brucei

mSphere, 2019
Ribosomal maturation is a complex and highly conserved biological process involving migration of a continuously changing RNP across multiple cellular compartments.
Constance Rink, Martin Ciganda, Noreen Williams   +2 more
doaj   +1 more source

Evolution of protein-RNA interactions

Current Opinion in Structural Biology
14 pages, 3 ...
Michal H. Kolář, Klára Hlouchová
openaire   +4 more sources