Results 41 to 50 of about 287,910 (304)

Molecular insights into substrate recognition and discrimination by the N-terminal domain of Lon AAA+ protease

eLife, 2021
The Lon AAA+ protease (LonA) is a ubiquitous ATP-dependent proteolytic machine, which selectively degrades damaged proteins or native proteins carrying exposed motifs (degrons).
Shiou-Ru Tzeng, Yin-Chu Tseng, Chien-Chu Lin, Chia-Ying Hsu, Shing-Jong Huang, Yi-Ting Kuo, Chung-I Chang   +6 more
doaj   +1 more source

Production of prone‐to‐aggregate proteins [PDF]

FEBS Letters, 2013
Expression of recombinant proteins inEscherichia coli(E. coli) remains the most popular and cost‐effective method for producing proteins in basic research and for pharmaceutical applications. Despite accumulating experience and methodologies developed over the years, production of recombinant proteins prone to aggregate inE.
Lebendiker, Mario, Danieli, Tsafi
openaire   +2 more sources

Particle Detection and Characterization for Biopharmaceutical Applications: Current Principles of Established and Alternative Techniques

Pharmaceutics, 2020
Detection and characterization of particles in the visible and subvisible size range is critical in many fields of industrial research. Commercial particle analysis systems have proliferated over the last decade.
Julia Gross-Rother, Michaela Blech, Eduard Preis, Udo Bakowsky, Patrick Garidel   +4 more
doaj   +1 more source

Interaction between Astaxanthin and Whey Protein Based on Fluorescence and Ultraviolet Spectroscopy

Shipin gongye ke-ji, 2022
The molecule self-assembly technology was used to prepare astaxanthin/whey protein nanocomplex, and the interaction mechanism between astaxanthin (AST) and whey protein was studied.
Yingyuan ZHAO, Shengmeng ZHANG, Yifan LI, Jin LIANG, Huihui JIA, Ziwei LI, Junxia LIU, Ruifang LI   +7 more
doaj   +1 more source

Aggrephagy: Selective Disposal of Protein Aggregates by Macroautophagy [PDF]

, 2012
Protein aggregation is a continuous process in our cells. Some proteins aggregate in a regulated manner required for different vital functional processes in the cells whereas other protein aggregates result from misfolding caused by various stressors ...
Lamark, Trond, Trond Lamark, Terje Johansen, Johansen, Terje   +3 more
core   +1 more source

Zoom in on Antibody Aggregates: A Potential Pitfall in the Search of Rare EV Populations

Biomedicines, 2021
High-resolution flow cytometers (hFCM) are used for the detection of extracellular vesicles (EV) in various biological fluids. Due to the increased sensitivity of hFCM, new artifacts with the potential of interfering with data interpretation are ...
Rikke W. Rasmussen, Jaco Botha, Frederik Prip, Mathilde Sanden, Morten H. Nielsen, Aase Handberg   +5 more
doaj   +1 more source

Distal renal tubules are deficient in aggresome formation and autophagy upon aldosterone administration.

PLoS ONE, 2014
Prolonged elevations of plasma aldosterone levels are associated with renal pathogenesis. We hypothesized that renal distress could be imposed by an augmented aldosterone-induced protein turnover challenging cellular protein degradation systems of the ...
Muhammad Umar Cheema, Helle Hasager Damkier, Jakob Nielsen, Ebbe Toftgaard Poulsen, Jan J Enghild, Robert A Fenton, Jeppe Praetorius   +6 more
doaj   +1 more source

The Molecular and Functional Interaction Between Membrane-Bound Organelles and Membrane-Less Condensates

Frontiers in Cell and Developmental Biology, 2022
A major recent advance in cell biology is the mechanistic and kinetic understanding of biogenesis of many membrane-less condensates. As membrane-less condensates and membrane-bound organelles are two major approaches used by the eukaryotic cells to ...
Chuankai Zhou, Chuankai Zhou
doaj   +1 more source

Pathogenic polyglutamine tracts are potent inducers of spontaneous Sup35 and Rnq1 amyloidogenesis. [PDF]

PLoS ONE, 2010
The glutamine/asparagine (Q/N)-rich yeast prion protein Sup35 has a low intrinsic propensity to spontaneously self-assemble into ordered, beta-sheet-rich amyloid fibrils.
Heike Goehler, Anja Dröge, Rudi Lurz, Sigrid Schnoegl, Yury O Chernoff, Erich E Wanker   +5 more
doaj   +1 more source

Protein aggregates.

, 2018
Representative cell for t = 6 with the maximum intensity projection at the top and the 45° 3D view with the ROI isolated from the whole cell at the bottom.
Rensu P. Theart (5698226), Ben Loos (590100), Yigael S. L. Powrie (5698229), Thomas R. Niesler (5698232)   +3 more
core   +1 more source