Aggregation of polyQ proteins is increased upon yeast aging and affected by Sir2 and Hsf1: novel quantitative biochemical and microscopic assays. [PDF]
PLoS ONE, 2012 Aging-related neurodegenerative disorders, such as Parkinson's, Alzheimer's and Huntington's diseases, are characterized by accumulation of protein aggregates in distinct neuronal cells that eventually die. In Huntington's disease, the protein huntingtin
Aviv Cohen +3 more
doaj +1 more source
Aggregation is a Context-Dependent Constraint on Protein Evolution
Frontiers in Molecular Biosciences, 2021 Solubility is a requirement for many cellular processes. Loss of solubility and aggregation can lead to the partial or complete abrogation of protein function. Thus, understanding the relationship between protein evolution and aggregation is an important
Michele Monti +9 more
doaj +1 more source
Lipid oxidation in foods and its implications on proteins
Frontiers in Nutrition, 2023 Lipids in foods are sensitive to various environmental conditions. Under light or high temperatures, free radicals could be formed due to lipid oxidation, leading to the formation of unstable food system.
Lianxin Geng +3 more
doaj +1 more source
Global analysis of protein aggregation in yeast during physiological conditions and arsenite stress
Biology Open, 2014 Protein aggregation is a widespread phenomenon in cells and associated with pathological conditions. Yet, little is known about the rules that govern protein aggregation in living cells. In this study, we biochemically isolated aggregation-prone proteins
Sebastian Ibstedt +3 more
doaj +1 more source
An in vivo platform to select and evolve aggregation-resistant proteins
Nature Communications, 2020 Protein aggregation remains a significant challenge for manufacturing of protein biopharmaceuticals. Here, the authors demonstrate the use of directed evolution and an assay for in vivo innate protein aggregation-propensity to generate aggregation ...
Jessica S. Ebo +14 more
doaj +1 more source
A3DyDB: exploring structural aggregation propensities in the yeast proteome
Microbial Cell Factories, 2023 Background The budding yeast Saccharomyces cerevisiae (S. cerevisiae) is a well-established model system for studying protein aggregation due to the conservation of essential cellular structures and pathways found across eukaryotes.
Javier Garcia-Pardo +6 more
doaj +1 more source
Frontiers in Bioengineering and Biotechnology, 2023 Protein aggregation is a major challenge in the development of therapeutic monoclonal antibodies (mAbs). Several stressors can cause protein aggregation, including temperature shifts, mechanical forces, freezing-thawing cycles, oxidants, reductants, and ...
Qiang Wu +11 more
doaj +1 more source
Why and how protein aggregation has to be studied
Microbial Cell Factories, 2013 The understanding of protein aggregation is a central issue in different fields of protein science, from the heterologous protein production in biotechnology to amyloid aggregation in several neurodegenerative and systemic diseases.
Ami Diletta +3 more
doaj +1 more source
An evolutionary trade-off between protein turnover rate and protein aggregation favors a higher aggregation propensity in fast degrading proteins. [PDF]
PLoS Computational Biology, 2011 We previously showed the existence of selective pressure against protein aggregation by the enrichment of aggregation-opposing 'gatekeeper' residues at strategic places along the sequence of proteins.
Greet De Baets +5 more
doaj +1 more source
Dynamic fluctuations of protein-carbohydrate interactions promote protein aggregation. [PDF]
PLoS ONE, 2009 Protein-carbohydrate interactions are important for glycoprotein structure and function. Antibodies of the IgG class, with increasing significance as therapeutics, are glycosylated at a conserved site in the constant Fc region.
Vladimir Voynov +8 more
doaj +1 more source