Results 21 to 30 of about 737,709 (296)
Heterologous prion-forming proteins interact to cross-seed aggregation in Saccharomyces cerevisiae [PDF]
, 2017 The early stages of protein misfolding remain incompletely understood, as most mammalian proteinopathies are only detected after irreversible protein aggregates have formed.
Keefer, Kathryn M +2 more
core +2 more sources
Protein aggregation in silico [PDF]
Trends in Biotechnology, 2007 Protein aggregation is a challenge to the successful manufacture of protein therapeutics; it can impose severe limitations on purification yields and compromise formulation stability. Advances in computer power, and the wealth of computational studies pertaining to protein folding, have facilitated the development of molecular simulation as a tool to ...
Troy, Cellmer +3 more
openaire +2 more sources
Lipid oxidation in foods and its implications on proteins
Frontiers in Nutrition, 2023 Lipids in foods are sensitive to various environmental conditions. Under light or high temperatures, free radicals could be formed due to lipid oxidation, leading to the formation of unstable food system.
Lianxin Geng +3 more
doaj +1 more source
The aggregation of cytochrome C may be linked to its flexibility during refolding [PDF]
, 2016 Large-scale expression of biopharmaceutical proteins in cellular hosts results in production of large insoluble mass aggregates. In order to generate functional product, these aggregates require further processing through refolding with denaturant, a ...
A Basu +19 more
core +1 more source
Detergent-mediated protein aggregation [PDF]
Chemistry and Physics of Lipids, 2013 Because detergents are commonly used to solvate membrane proteins for structural evaluation, much attention has been devoted to assessing the conformational bias imparted by detergent micelles in comparison to the native environment of the lipid bilayer.
Chris, Neale +5 more
openaire +2 more sources
An in vivo platform to select and evolve aggregation-resistant proteins
Nature Communications, 2020 Protein aggregation remains a significant challenge for manufacturing of protein biopharmaceuticals. Here, the authors demonstrate the use of directed evolution and an assay for in vivo innate protein aggregation-propensity to generate aggregation ...
Jessica S. Ebo +14 more
doaj +1 more source
O-GlcNAc modification blocks the aggregation and toxicity of the protein α-synuclein associated with Parkinson's disease. [PDF]
, 2015 Several aggregation-prone proteins associated with neurodegenerative diseases can be modified by O-linked N-acetyl-glucosamine (O-GlcNAc) in vivo. One of these proteins, α-synuclein, is a toxic aggregating protein associated with synucleinopathies ...
Ambroso, Mark R +8 more
core +2 more sources
Protein aggregation and neurodegeneration [PDF]
Methods, 2011 In 1997 Carrell and Lomas put forth the concept that many disorders, which appeared to be unlinked, arose from the same general mechanism that involved the abnormal unfolding and aggregation of various underlying proteins [1]. The flux of proteins through pathways for formation of amyloid-like aggregates was proposed to be the mechanism for ...
Katie J, Mayo, Douglas M, Cyr
openaire +2 more sources
A3DyDB: exploring structural aggregation propensities in the yeast proteome
Microbial Cell Factories, 2023 Background The budding yeast Saccharomyces cerevisiae (S. cerevisiae) is a well-established model system for studying protein aggregation due to the conservation of essential cellular structures and pathways found across eukaryotes.
Javier Garcia-Pardo +6 more
doaj +1 more source
Frontiers in Bioengineering and Biotechnology, 2023 Protein aggregation is a major challenge in the development of therapeutic monoclonal antibodies (mAbs). Several stressors can cause protein aggregation, including temperature shifts, mechanical forces, freezing-thawing cycles, oxidants, reductants, and ...
Qiang Wu +11 more
doaj +1 more source