Results 31 to 40 of about 436,505 (303)
An in vivo platform to select and evolve aggregation-resistant proteins
Nature Communications, 2020 Protein aggregation remains a significant challenge for manufacturing of protein biopharmaceuticals. Here, the authors demonstrate the use of directed evolution and an assay for in vivo innate protein aggregation-propensity to generate aggregation ...
Jessica S. Ebo +14 more
doaj +1 more source
A3DyDB: exploring structural aggregation propensities in the yeast proteome
Microbial Cell Factories, 2023 Background The budding yeast Saccharomyces cerevisiae (S. cerevisiae) is a well-established model system for studying protein aggregation due to the conservation of essential cellular structures and pathways found across eukaryotes.
Javier Garcia-Pardo +6 more
doaj +1 more source
Global analysis of protein aggregation in yeast during physiological conditions and arsenite stress
Biology Open, 2014 Protein aggregation is a widespread phenomenon in cells and associated with pathological conditions. Yet, little is known about the rules that govern protein aggregation in living cells. In this study, we biochemically isolated aggregation-prone proteins
Sebastian Ibstedt +3 more
doaj +1 more source
Frontiers in Bioengineering and Biotechnology, 2023 Protein aggregation is a major challenge in the development of therapeutic monoclonal antibodies (mAbs). Several stressors can cause protein aggregation, including temperature shifts, mechanical forces, freezing-thawing cycles, oxidants, reductants, and ...
Qiang Wu +11 more
doaj +1 more source
Folding and aggregation of designed proteins [PDF]
Proceedings of the National Academy of Sciences, 1998 Protein aggregation is studied by following the simultaneous folding of two designed identical 20-letter amino acid chains within the framework of a lattice model and using Monte Carlo simulations. It is found that protein aggregation is determined by elementary structures (partially folded intermediates) controlled by local contacts among some of the ...
Broglia R. A. +4 more
openaire +3 more sources
Why and how protein aggregation has to be studied
Microbial Cell Factories, 2013 The understanding of protein aggregation is a central issue in different fields of protein science, from the heterologous protein production in biotechnology to amyloid aggregation in several neurodegenerative and systemic diseases.
Ami Diletta +3 more
doaj +1 more source
Production of prone‐to‐aggregate proteins [PDF]
FEBS Letters, 2013 Expression of recombinant proteins inEscherichia coli(E. coli) remains the most popular and cost‐effective method for producing proteins in basic research and for pharmaceutical applications. Despite accumulating experience and methodologies developed over the years, production of recombinant proteins prone to aggregate inE.
Lebendiker, Mario, Danieli, Tsafi
openaire +2 more sources
An evolutionary trade-off between protein turnover rate and protein aggregation favors a higher aggregation propensity in fast degrading proteins. [PDF]
PLoS Computational Biology, 2011 We previously showed the existence of selective pressure against protein aggregation by the enrichment of aggregation-opposing 'gatekeeper' residues at strategic places along the sequence of proteins.
Greet De Baets +5 more
doaj +1 more source
The RNA-Binding Protein Scp160p Facilitates Aggregation of Many Endogenous Q/N-Rich Proteins
Cell Reports, 2018 Summary: The RNA-binding protein Scp160p is the yeast homolog of the conserved vigilin protein family. These proteins influence a variety of nuclear and cytoplasmic functions.
Matthew H.K. Cheng +6 more
doaj +1 more source
AGGRESCAN: a server for the prediction and evaluation of "hot spots" of aggregation in polypeptides
BMC Bioinformatics, 2007 Background Protein aggregation correlates with the development of several debilitating human disorders of growing incidence, such as Alzheimer's and Parkinson's diseases.
Vendrell Josep +5 more
doaj +1 more source