Results 101 to 110 of about 2,014,238 (321)

A bead-based western for high-throughput cellular signal transduction analyses

Nature Communications, 2016
Dissecting cellular signalling requires the analysis of large numbers of proteins. Here the authors describe DigiWest, a high-throughput protein detection method that combines the concept of western and widely-used bead array systems that allows rapid ...
Fridolin Treindl, Benjamin Ruprecht, Yvonne Beiter, Silke Schultz, Anette Döttinger, Annette Staebler, Thomas O. Joos, Simon Kling, Oliver Poetz, Tanja Fehm, Hans Neubauer, Bernhard Kuster, Markus F. Templin   +12 more
doaj   +1 more source

The photoswitchable cannabinoid azo‐HU308 enables optical control of Ca2+ dynamics in INS‐1 β‐cells via off‐target effects on TRPC channels

FEBS Open Bio, EarlyView.
Light activation of the photoswitchable cannabinoid ligand azo‐HU308 triggers Ca2+ influx in pancreatic β‐cells through TRPC channels, independent of CB2 cannabinoid receptors. This reveals a non‐GPCR pathway for cannabinoid modulation of β‐cell Ca2+ dynamics and establishes azo‐HU308 as an optical tool to study cannabinoid signaling through TRP ...
Alexander E. G. Viray, James A. Frank
wiley   +1 more source

Analysis of Treacher Collins syndrome 4‐associated mutations in Schizosaccharomyces pombe

FEBS Open Bio, EarlyView.
Fission yeast models carrying Treacher Collins syndrome type 4‐associated mutations reveal that impaired processivity of RNA polymerase I leads to defective rRNA transcription. This study highlights the essential role of a conserved arginine residue in Pol I elongation and provides mechanistic insight into the pathogenesis of ribosomopathies.
Kei Kawakami, Hiroaki Kato
wiley   +1 more source

From research to clinical application multi-parameter testing: Marker panels for the early detection of complex diseases [PDF]

Journal of Medical Biochemistry, 2009
Multi Parameter Analysis can open novel diagnostic opportunities for the early diagnosis and screening of multimodal diseases like cancer. Single proteins have so far failed to describe such complex diseases.
Wild Norbert, Karl Johann, Risse Bernhard   +2 more
doaj  

A Label-Free Fluorescent Array Sensor Utilizing Liposome Encapsulating Calcein for Discriminating Target Proteins by Principal Component Analysis

Sensors, 2017
A new fluorescent arrayed biosensor has been developed to discriminate species and concentrations of target proteins by using plural different phospholipid liposome species encapsulating fluorescent molecules, utilizing differences in permeation of the ...
Ryota Imamura, Naoki Murata, Toshinori Shimanouchi, Kaoru Yamashita, Masayuki Fukuzawa, Minoru Noda   +5 more
doaj   +1 more source

Establishment of a coculture system for Porphyromonas gingivalis and head and neck squamous cell carcinoma using spheroid culture and LATS inhibition

FEBS Open Bio, EarlyView.
We established a spheroid coculture system enabling viable Porphyromonas gingivalis–HNSCC interactions under normoxic conditions. Inhibition of LATS1/2 maintains tumor cells in an undifferentiated state, which may promote spheroid growth and create a more permissive environment for bacterial persistence.
Yurika Nakajima, Shogo Okazaki, Muneaki Tamura, Shuichi Sato, Kenichi Imai   +4 more
wiley   +1 more source

Efficiency of Disease and Disease Activity Diagnosis Models of Systemic Lupus Erythematosus Based on Protein Array Analysis. [PDF]

J Immunol Res, 2022
Zhao Y, Qi Y, Liu X, Cui Y, Zhao Z.
europepmc   +1 more source

A Pneumococcal Protein Array as a Platform to Discover Serodiagnostic Antigens Against Infection*

Molecular & Cellular Proteomics, 2015
Pneumonia is one of the most common and severe diseases associated with Streptococcus pneumoniae infections in children and adults. Etiological diagnosis of pneumococcal pneumonia in children is generally challenging because of limitations of diagnostic ...
A. Olaya-Abril, I. Jiménez-Munguía, L. Gómez-Gascón, I. Obando, M. Rodríguez-Ortega   +4 more
semanticscholar   +1 more source

The effect of salubrinal on the endoplasmic reticulum stress pathway in heat‐stressed spermatogonial cells in vitro

FEBS Open Bio, EarlyView.
This study investigates the protective role of salubrinal against heat‐induced endoplasmic reticulum (ER) stress in mouse spermatogenic cells (GC1 and GC2). By modulating the ER stress pathway, salubrinal alleviates cellular stress and supports spermatogenic cell survival, suggesting its potential as a therapeutic candidate for heat‐related infertility.
Suna Karadeniz Saygili, Mustafa Oztatlici, Mahmut Kemal Ozbilgin   +2 more
wiley   +1 more source

Chemical-genetic profile analysis in yeast suggests that a previously uncharacterized open reading frame, YBR261C, affects protein synthesis

BMC Genomics, 2008
Background Functional genomics has received considerable attention in the post-genomic era, as it aims to identify function(s) for different genes. One way to study gene function is to investigate the alterations in the responses of deletion mutants to ...
Eroukova Veronika, Alamgir Md, Jessulat Matthew, Xu Jianhua, Golshani Ashkan   +4 more
doaj   +1 more source