High-throughput analysis and protein engineering using microcapillary arrays [PDF]
Nature Chemical Biology, 2015 We describe a multipurpose technology platform, termed μSCALE (microcapillary single-cell analysis and laser extraction), that enables massively parallel, quantitative biochemical and biophysical measurements on millions of protein variants expressed from yeast or bacteria.
Bob Chen +8 more
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Antibody Protein Array Analysis of the Tear Film Cytokines [PDF]
Optometry and Vision Science, 2008 Many bioactive proteins including cytokines are reported to increase in dry eye disease although the specific profile and concentration of inflammatory mediators varies considerably from study to study. In part, this variability results from inherent difficulties in quantifying low abundance proteins in a limited sample volume using relatively low ...
Shimin Li +7 more
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Proteomics analysis of vesicles isolated from plasma and urine of prostate cancer patients using a multiplex, aptamer‐based protein array [PDF]
Journal of Extracellular Vesicles, 2016 Proteomics analysis of biofluid-derived vesicles holds enormous potential for discovering non-invasive disease markers. Obtaining vesicles of sufficient quality and quantity for profiling studies has, however, been a major problem, as samples are often ...
Joanne L. Welton +10 more
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PLoS ONE, 2010 Background Dominant mutations in leucine-rich repeat kinase 2 (LRRK2) are the most common genetic cause of Parkinson's disease, however, the underlying pathogenic mechanisms are poorly understood.
Susanne Zach +2 more
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RPPanalyzer: Analysis of reverse-phase protein array data [PDF]
Bioinformatics, 2010 Abstract Summary: RPPanalyzer is a statistical tool developed to read reverse-phase protein array data, to perform the basic data analysis and to visualize the resulting biological information. The R-package provides different functions to compare protein expression levels of different samples and to normalize the data.
Mannsperger, Heiko A. +4 more
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Biphasic Effect of Curcumin on Morphine Tolerance: A Preliminary Evidence from Cytokine/Chemokine Protein Array Analysis [PDF]
Evidence-Based Complementary and Alternative Medicine, 2010 The aim of this study was to evaluate the effect of curcumin on morphine tolerance and the corresponding cytokine/chemokine changes. Male ICR mice were made tolerant to morphine by daily subcutaneous injection for 7 days.
Jui‐An Lin +10 more
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Modified SuperCurve Method for Analysis of Reverse-Phase Protein Array Data [PDF]
Journal of Computational Biology, 2015 Reverse-phase protein arrays (RPPAs) are widely used in biological and biomedical fields of study. One of the most popular analytic methods in RPPA data analysis is the SuperCurve method, which requires estimation of the background fluorescence level. This estimation is usually not accurate and has sample bias and spatial bias.
Miao Sun +3 more
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Novel computational analysis of protein binding array data identifies direct targets of
BMC Bioinformatics, 2011 Background The creation of a complete genome-wide map of transcription factor binding sites is essential for understanding gene regulatory networks in vivo.
Kaestner Klaus H +4 more
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Cluster analysis of protein array results via similarity of Gene Ontology annotation
BMC Bioinformatics, 2006 Background With the advent of high-throughput proteomic experiments such as arrays of purified proteins comes the need to analyse sets of proteins as an ensemble, as opposed to the traditional one-protein-at-a-time approach.
Wolting Cheryl +2 more
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Protein expression profiling arrays: tools for the multiplexed high-throughput analysis of proteins. [PDF]
Proteome science, 2003 The completion of the human genome sequence has led to a rapid increase in genetic information. The invention of DNA microarrays, which allow for the parallel measurement of thousands of genes on the level of mRNA, has enabled scientists to take a more global view of biological systems.
Jens Sydor, Steffen Nock
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