Results 21 to 30 of about 2,677,114 (323)
High-throughput analysis and protein engineering using microcapillary arrays [PDF]
Nature Chemical Biology, 2015 We describe a multipurpose technology platform, termed μSCALE (microcapillary single-cell analysis and laser extraction), that enables massively parallel, quantitative biochemical and biophysical measurements on millions of protein variants expressed from yeast or bacteria.
Bob Chen +8 more
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Scientific Reports, 2022 Protein–protein interaction (PPI) analysis is a key process to understand protein functions. Recently, we constructed a human protein array (20 K human protein beads array) consisting of 19,712 recombinant human proteins produced by a wheat cell-free ...
Shusei Sugiyama +6 more
doaj +1 more source
Reverse-Phase Protein Array: Technology, Application, Data Processing, and Integration.
Journal of Biomolecular Techniques, 2021 Reverse-phase protein array (RPPA) is a high-throughput antibody-based targeted proteomics platform that can quantify hundreds of proteins in thousands of samples derived from tissue or cell lysates, serum, plasma, or other body fluids.
C. Coarfa +9 more
semanticscholar +1 more source
In situ structural analysis of Golgi intracisternal protein arrays [PDF]
Proceedings of the National Academy of Sciences, 2015 Significance To our knowledge, this is the first detailed study of Golgi ultrastructure within unperturbed cells. Three intracisternal structures were identified, with implications for Golgi architecture and trafficking: ( i ) Bundles of filaments show how cargoes may ...
Engel, Benjamin D. +5 more
openaire +5 more sources
Viruses, 2015 Introduction: Comprehensive evaluation of measles-specific humoral immunity after vaccination is important for determining new and/or additional correlates of vaccine immunogenicity and efficacy.
Iana H. Haralambieva +6 more
doaj +1 more source
Pro-MAP: a robust pipeline for the pre-processing of single channel protein microarray data
BMC Bioinformatics, 2022 Background The central role of proteins in diseases has made them increasingly attractive as therapeutic targets and indicators of cellular processes. Protein microarrays are emerging as an important means of characterising protein activity.
Metoboroghene Oluwaseyi Mowoe +6 more
doaj +1 more source
Doubly stochastic continuous-time hidden Markov approach for analyzing genome tiling arrays [PDF]
, 2008 Microarrays have been developed that tile the entire nonrepetitive genomes of many different organisms, allowing for the unbiased mapping of active transcription regions or protein binding sites across the entire genome.
Johnson, W. Evan +2 more
core +1 more source
A Network-based Analysis of Polyanion-binding Proteins Utilizing Yeast Protein Arrays [PDF]
Molecular & Cellular Proteomics, 2006 The high affinity of certain cellular polyanions for many proteins (polyanion-binding proteins (PABPs)) has been demonstrated previously. It has been hypothesized that such polyanions may be involved in protein structure stabilization, stimulation of folding through chaperone-like activity, and intra- and extracellular protein transport as well as ...
Salamat-Miller, N. +6 more
openaire +3 more sources
Toxics, 2023 Air pollutants are associated with exacerbations of asthma, chronic bronchitis, and airway inflammation. Diesel exhaust particles (DEPs) can induce and worsen lung diseases.
Byeong-Gon Kim +3 more
doaj +1 more source
A Network-based Analysis of Polyanion-binding Proteins Utilizing Human Protein Arrays [PDF]
Journal of Biological Chemistry, 2007 The existence of interactions between many cellular proteins and various polyanionic surfaces within a cell is now well established. The functional role of such interactions, however, remains to be clearly defined. The existence of protein arrays, with a large selection of different kinds of proteins, provides a way to better address a number of ...
Salamat-Miller, N. +5 more
openaire +3 more sources