Results 71 to 80 of about 25,791,466 (309)

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

DisCoTune: versatile auxiliary plasmids for the production of disulphide‐containing proteins and peptides in the E. coli T7 system

Microbial Biotechnology, 2021
Summary Secreted proteins and peptides hold large potential both as therapeutics and as enzyme catalysts in biotechnology. The high stability of many secreted proteins helps maintain functional integrity in changing chemical environments and is a ...
Andreas B. Bertelsen, Celeste Menuet Hackney, Carolyn N. Bayer, Lau D. Kjelgaard, Maja Rennig, Brian Christensen, Esben Skipper Sørensen, Helena Safavi‐Hemami, Tune Wulff, Lars Ellgaard, Morten H. H. Nørholm   +10 more
doaj   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

Exploring protein–protein interactions and oligomerization state of pulmonary surfactant protein C (SP-C) through FRET and fluorescence self-quenching

, 2023
Acknowledgments: FPI fellowship from the Spanish Ministry of Science and Innovation. COMPETE2020-Operational Program for Competitiveness and Internationalization, funding by the European Regional Development Fund.
Prieto, Manuel, Coutinho, Ana, Pérez Gil, Jesús, Morán Lalangui, Juranny Michelle, García Álvarez, María Begoña, Fedorov, Alexander, Loura, Luís M. S.   +6 more
core   +1 more source

Monomeric C-Reactive Protein in Serum With Markedly Elevated CRP Levels Shares Common Calcium-Dependent Ligand Binding Properties With an in vitro Dissociated Form of C-Reactive Protein [PDF]

, 2020
A monomeric form of C-reactive protein (CRP) which precipitates with cell wall pneumococcal C polysaccharide (CWPS) and retains the ability to reversibly bind to its ligand phosphocholine has been produced through urea-induced dissociation at an ...
Fryer, Anthony A., Moran, Jennifer A., Williams, Robert D., Shrive, Annette K., Littlejohn, Jamie R., Williams, Harry M., Greenhough, Trevor J.   +6 more
core   +1 more source

Organizing the interface—Plasma membrane architecture and receptor dynamics in virus‐cell interactions

FEBS Letters, EarlyView.
Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley   +1 more source

C-reactive protein is essential for innate resistance to pneumococcal infection. [PDF]

, 2014
No deficiency of human C-reactive protein (CRP), or even structural polymorphism of the protein, has yet been reported so its physiological role is not known.
Lawrence, Rachel A., Read, Kevin D., Aviva Petrie, Pepys, M B, Pepys, MB, Hutchinson, Winston L., LSHTM, Read, Kevin D, Raynes, JG, Read, KD, J. Brian Souza, Tennent, G A, Stephan Ellmerich, Loeffler, JM, Simons, JP, Rachel A. Lawrence, Simons, J Paul, Tennent, GA, John G. Raynes, Dundee, Glenys A. Tennent, Ellmerich, Stephan, Ellmerich, S, Tennent, Glenys A, Al-Shawi, Raya, Petrie, A, Tennent, Glenys A., Simons, J. Paul, Lawrence, R A, Jutta M. Loeffler, Lawrence, RA, Hutchinson, Winston L, J. Paul Simons, de Souza, J Brian, de Souza, J. Brian, Kevin D. Read, Raynes, John G., Raynes, J G, Read, K D, Mark B. Pepys, Hutchinson, WL, de Souza, JB, Loeffler, Jutta M, Petrie, Aviva, Al-Shawi, R, Pepys, Mark B., Lawrence, Rachel A, Raynes, John G, Loeffler, J M, de Souza, J B, Hutchinson, W L, Pepys, Mark B, Raya Al‐Shawi, Winston L. Hutchinson, Loeffler, Jutta M., Simons, J P, UCL   +56 more
core   +1 more source

Changes in plasma hemostatic parameters under intravascular laser irradiation of blood in patients with community-acquired pneumonia

Терапевтический архив, 2010
Aim. To study the time course of changes in the activity of the protein C system and other hemostatic parameters under intravascular laser irradiation of blood (ILIB) in patients with community-acquired pneumonia (CAP). Subjects and methods.
Nikolay Mikhaylovich Burduli, Nana Guramovna Pilieva, N M Burduli, N G Piliyeva   +3 more
doaj  

Biophysical approaches for studying viral entry

FEBS Letters, EarlyView.
Viruses infect all living organisms and have been responsible for major epidemics and pandemics. Their ongoing evolutionary battle with host defenses creates a constant need for improved tools to study viral behavior. Advancing methods to probe viral attachment, fusion, and genome release deepen our understanding of how infections begin and support the
Inbar Yosibash, Raya Sorkin
wiley   +1 more source