Results 101 to 110 of about 568,309 (314)

Protein Expression, Isolation And Purification

, 2016
Authors: Creative ...
openaire   +2 more sources

a-Synuclein protein expression and purification v1

, 2022
Protocol for recombinant a-synuclein purification, useful as monomer template for seeded-amplification assays (like RT_QUIC or PMCA) . Recommendations are to store the protein always on ice while not running and do not stop purification when it is started.
andrew.west not provided, arpine.sokratian not provided   +1 more
openaire   +1 more source

Purification of the Campylobacter jejuni Dps protein assisted by its high melting temperature

, 2015
Dps proteins (DNA binding protein from starved cell) form a distinct group within the ferritin superfamily. All Dps members are composed of 12 identical subunits that assemble into a conserved spherical protein shell.
Korolik, Victoria, Souza, Emanuel, Moure, Vivian, Pedrosa, Fábio, Moure, Vivian R, Souza, Emanuel M, Huergo, Luciano, Valdameri, Glaucio, Sanchuki, Heloisa BS, Oliveira, Marco A, Huergo, Luciano F, Sanchuki, Heloisa, Pedrosa, Fabio O, Oliveira, Marco   +13 more
core   +1 more source

Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates

FEBS Letters, EarlyView.
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley   +1 more source

Expression and purification of catalytically active human PHD3 in Escherichia coli.

, 2007
Transcription factor HIF-1 is a key regulator in cellular adaptation to hypoxia. HIF prolyl hydroxylases (PHDs) control HIF-1 accumulation by hydroxylation dependent on molecular oxygen.
Fedulova, Natalia, Hanrieder, Jörg,, Bergquist, Jonas, Emrén, Lars O.,, Hanrieder, J\uf6rg, Emrén, Lars O, Emr\ue9n, Lars O, Fedulova, Natalia,, Bergquist, Jonas,   +8 more
core   +1 more source

High-throughput strategy for identification of Mycobacterium tuberculosis membrane protein expression conditions using folding reporter GFP

, 2022
Mycobacterium tuberculosis membrane protein biochemistry and structural biology studies are often hampered by challenges in protein expression and selection for well-expressing protein candidates, suitable for further investigation.
Bennett, Matthew D.,, Grāve, Kristīne,, Högbom, Martin,   +2 more
core   +1 more source

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

FEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens, Brendan W. Chandler, Anna M. Schmoker, Jaye L. Weinert, Charlotte A. Kearns, Warren T. Yacawych, Amila Šemić, Alicia M. Ebert, Bryan A. Ballif   +8 more
wiley   +1 more source

Recombinant Protein Expression and Purification of N, S1, and RBD of SARS-CoV-2 from Mammalian Cells and Their Potential Applications. [PDF]

Diagnostics (Basel), 2021
García-Cordero J, Mendoza-Ramírez J, Fernández-Benavides D, Roa-Velazquez D, Filisola-Villaseñor J, Martínez-Frías SP, Sanchez-Salguero ES, Miguel-Rodríguez CE, Maravillas Montero JL, Torres-Ruiz JJ, Gómez-Martín D, Argumedo LS, Morales-Ríos E, Alvarado-Orozco JM, Cedillo-Barrón L.   +14 more
europepmc   +1 more source

Bax to the future : A novel, high-yielding approach for purification and expression of full-length Bax protein for structural studies

, 2019
Mitochondria-mediated apoptosis (programmed cell death) involves a sophisticated signaling and regulatory network that is regulated by the Bcl-2 protein family. Members of this family have either pro- or anti-apoptotic functions.
Stoll, Raphael,, Gröbner, Gerhard,, Lindberg, Mikael J.,, Ådén, Jörgen,, Dingeldein, Artur Peter Günther,, Zhong, Xueyin,   +5 more
core   +1 more source

Mixed‐class J‐domain protein scaffolds promote expanded aggregate handling and multivalent Hsp70 engagement during functional disaggregase assembly

FEBS Letters, EarlyView.
Protein aggregates threaten proteostasis and cell health. In human cells, Hsp70–J‐domain protein‐based disaggregases remove aggregates, but how they assemble remains unclear. Our biochemical findings show that DNAJA2‐ and DNAJB1‐containing disaggregase scaffolds enhance luciferase aggregate targeting, and that Hsp70 recruitment by both J‐domain ...
Anna Szlachcic, Nadinath B. Nillegoda
wiley   +1 more source