Results 81 to 90 of about 568,309 (314)

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

pH‐mediated activation of the lysosomal arginine sensor SLC38A9

FEBS Letters, EarlyView.
Cells monitor nutrient levels via the lysosomal transporter SLC38A9 to activate the mechanistic target of rapamycin complex 1 (mTORC1). This study reveals that SLC38A9 function is regulated by pH. We identified histidine 544 as a critical pH sensor that undergoes conformational changes to control amino acid efflux from lysosomes; therefore, it ...
Xuelang Mu, Ampon Sae Her, Tamir Gonen
wiley   +1 more source

Protocol for in vitro phase separation of N terminus of CIDEC proteins

STAR Protocols
Summary: Cell death-inducing DFF45-like effector C (CIDEC) condensation through N-terminal phase separation is a prerequisite for CIDEC-mediated lipid droplet (LD) fusion and lipid storage promotion.
Yiming Lu, Zanzan Zhu, Jianqin Wang, Xuchao Lyu, Peng Li, Feng-Jung Chen   +5 more
doaj   +1 more source

Expression and purification of untagged GlnK proteins from actinobacteria

EXCLI journal, 2017
Fil: Huergo, Luciano F..
Gerhardt, Edileusa C.M., Moure, Vivian R., Souza, Andrey W., Pedrosa, Fabio O., Souza, Emanuel M., Diacovich, Lautaro, Gramajo, Hugo, Huergo, Luciano F.   +7 more
openaire   +4 more sources

Cloning, expression, and purification of the virulence-associated protein D from Xylella fastidiosa

, 2015
in this study, an efficient expression system, based on the pET32Xa/LIC vector, for producing a Xylella fastidiosa virulence-associated protein D, found to have a strong similarity to Riemerella anatipestifer and Actinobacillus actinomycetencomitans VapD
Catani, CF, Tada, SFS, Yano, T, de Souza, AP, Azzoni, AR, Paula, DP, Rosselli, LK   +6 more
core   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Protocol for detecting and quantifying hATF4-HA in non-stress versus stress conditions using automated and quantitative Jess western blotting

STAR Protocols
Summary: Activating transcription factor 4 (ATF4) is a key player in the integrated stress response, whose expression is subject to tight translational control. Studying its stress-provoked induction, accompanied by the general translational shutdown, is
Anna M. Smirnova, Anna Herrmannová, Leoš Shivaya Valášek   +2 more
doaj   +1 more source

Bacterial expression and purification of recombinant Plasmodium yoelii circumsporozoite protein

, 1997
We report the expression and purification of recombinant rodent malarialPlasmodium yoeliicircumsporozoite surface protein (PyCSP) inEscherichia coli.To facilitate purification of the recombinant protein, the PyCSP was expressed as an amino-terminal ...
Kang, A.S., Schmida, S.R., Stratmann, T., Harperb, J.F.   +3 more
core   +1 more source

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source

Protocol for producing brain-derived neurotrophic factor and neurotrophin-4 in their pro and active form in Escherichia coli

STAR Protocols
Summary: Brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NT4) are neurotrophic growth factors, signaling primarily via the tropomyosin receptor kinase B (TrkB).
Christoph Holzner, Katharina Böttinger, Christian G. Huber, Elfriede Dall, Hans Brandstetter   +4 more
doaj   +1 more source