Results 91 to 100 of about 568,309 (314)

Optimized protocol for expression and purification of membrane-bound PglB, a bacterial oligosaccharyl transferase

, 2013
Asparagine-linked glycosylation (NLG) plays a significant role in a diverse range of cellular processes, including protein signaling and trafficking, the immunologic response, and immune system evasion by pathogens.
Imperiali, Barbara, Jaffee, Marcie Beth
core   +1 more source

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

FEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

Protocol for the recombinant expression and purification of the LSAM domain of human legumain in E. coli

STAR Protocols
Summary: Expressing disulfide-rich proteins in E. coli is challenging due to incorrect bond formation. Here, we present a protocol for expressing the PC1pro-LSAM fusion protein in E.
Sven O. Dahms, Alexander C. Wieland, Hans Brandstetter, Elfriede Dall   +3 more
doaj   +1 more source

Growth hormone receptor agonists and antagonists: From protein expression and purification to long-acting formulations. [PDF]

Protein Sci, 2023
Wang Y, Kim M, Buckley C, Maynard HD, Langley RJ, Perry JK.   +5 more
europepmc   +1 more source

Electron transfer between complexes III and IV in S. cerevisiae mitochondrial membranes

FEBS Letters, EarlyView.
Mitochondrial oxidative phosphorylation in S. cerevisiae mitoplasts is limited by complex IV catalytic capacity, rather than two‐dimensional cytochrome c diffusion. At physiological cytochrome c : supercomplex ratios at salinity equivalent to that of 20 mm monovalent salt, activity is maximized, indicating that this low ionic strength accurately mimics
Ana Paula Lobez, Mikael Oliveberg, Peter Brzezinski   +2 more
wiley   +1 more source

Protein expression and purification of bioactive growth factors for use in cell culture and cellular agriculture. [PDF]

STAR Protoc, 2023
Semper C, Savchenko A.
europepmc   +1 more source

Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis

FEBS Letters, EarlyView.
Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee, Junho Jeong, Chae Yeong Lee, Hyunjoon Cho, Man Hwan Oh, Je Chul Lee, Jee‐Young Lee, Yangmee Kim   +7 more
wiley   +1 more source

Protocol for validating liquid-liquid phase separation as a driver of membraneless organelle assembly in vitro and in human cells

STAR Protocols
Summary: Liquid-liquid phase separation (LLPS) of scaffold proteins has often been proposed to drive the biogenesis of membraneless cellular compartments. Here, we present a protocol to link in vitro LLPS propensity to localization in vivo.
Marius Hedtfeld, Andrea Musacchio
doaj   +1 more source

Data on the optimizations of expression and purification of human BiP/GRP78 protein in Escherichia coli

Data in Brief, 2017
Human BiP/GRP78 is involved in the folding and assembly of proteins in the endoplasmic reticulum. The proteins for crystallization in good amount and quality are prerequisites for obtaining ideal crystals.
Jiao Yang, Lei Zhou, Qinglian Liu
doaj   +1 more source

Protein Expression and Purification of Romiplostim and Analysis of Its Secretory Production Using an In Silico Investigated Signal Peptide in E. Coli. [PDF]

Rep Biochem Mol Biol, 2023
Hashemzaei M, Negahdaripour M, Heidari R, Ghoshoon MB.   +3 more
europepmc   +1 more source