Results 71 to 80 of about 568,309 (314)

Trim66’s paternal deficiency causes intrauterine overgrowth

Life Science Alliance
TRIM66 is a PHD-Bromo–containing protein primarily expressed in spermatids. Here, we report a paternal effect phenotype whereby homozygous Trim66 -mutant males sired progeny overweight at birth.
Monika Mielnicka, Francesco Tabaro, Rahul Sureka, Basilia Acurzio, Renata Paoletti, Ferdinando Scavizzi, Marcello Raspa, Alvaro H Crevenna, Karine Lapouge, Kim Remans, Matthieu Boulard   +10 more
doaj   +1 more source

Protein Expression and Purification v1

, 2017

Xuehua Wan, not provided Jennifer A. Saito, not provided Shaobin Hou, not provided Maqsudul Alam   +3 more
openaire   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Expression and purification of recombinant full-length NS3 protease-helicase from a new variant of Hepatitis C virus

, 2002
Viral mRNA extracted from the serum of a patient infected with HCV strain I a was used for cloning, expression, and purification of full-length Hepatitis C NS3 protein.
Danielson, U Helena,, Stenberg, Gun,, Shuman, Cynthia F,, Poliakov, Anton,, Hubatsch, Ina,   +4 more
core   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Cloning, expression and purification of the luminal domain of spinach photosystem 1 subunit PsaF functional in binding to plastocyanin and with a disulfide bridge required for folding

, 2011
The photosystem 1 subunit PsaF is involved in the docking of the electron-donor proteins plastocyanin and cytochrome c6 in eukaryotic photosynthetic organisms.
Daniel Farkas, Hansson, Örjan,, Lars-Gunnar Franzén, Örjan Hansson, Farkas, Daniel,, Franzén, Lars-Gunnar,   +5 more
core   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Protein Expression and Purification [PDF]

, 1996
Texto completo. Acesso restrito. p. 355–366The azurophil granules of human PMN contain four antibiotic proteins, the serprocidins, which have extensive homology to one another and to serine proteases.
McCabe, Denise, Gabay, Joelle E., Almeida, Roque Pacheco de, Melchior, Maxine, Vanet, Anne, Witko-Sarsat, Veronique   +5 more
core  

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Expression, solubilisation, and purification of a functional CMP-sialic acid transporter in Pichia pastoris

, 2014
Membrane proteins, including solute transporters play crucial roles in cellular function and have been implicated in a variety of important diseases, and as such are considered important targets for drug development.
Maggioni, Andrea, Hadley, Barbara, von Itzstein, Mark, Tiralongo, Joe   +3 more
core   +1 more source