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Microfluidics for Protein Expression, Purification, and Screening
Bharadwaj, Rajiv +2 more
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Database Study on the Expression and Purification of Membrane Proteins
Protein & Peptide Letters, 2021Membrane proteins are crucial for biological processes, and many of them are important to drug targets. Understanding the three-dimensional structures of membrane proteins are essential to evaluate their bio-function and drug design. High-purity membrane proteins are important for structural determination.
Chen-Yan, Zhang +8 more
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Expression and purification of ataxin-1 protein
Journal of Neuroscience Methods, 2010Ataxin-1 is part of a larger family of polyglutamine-containing proteins that is linked to nine distinct neurodegenerative disorders. There are no known effective therapies for any of these expanded polyglutamine tract disorders. One possible reason for this is the lack of sufficient amounts of pure polyglutamine-containing proteins suitable for ...
Rhonda, Husain-Ponnampalam +3 more
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Expression, purification, and characterization of the TRIM49 protein
Protein Expression and Purification, 2018Autophagy is the process of degradation of intracellular proteins through the lysosome. Members of the tripartite motif (TRIM) proteins have shown to directly recognize autophagic cargo and also to act as a hub for the phagophore nucleation complex. The TRIM proteins are classically characterized by the presence of an amino-terminal RING domain and a B-
Dimitrius Santiago Guimarães +1 more
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Recombinant Expression and Purification of Smad Proteins
Protein Expression and Purification, 2000Smads transduce intracellular signals initiated by members of the transforming growth factor beta (TGF beta) family, including activins, TGF betas, and bone morphogenetic proteins. Recently, various models concerning the mechanism of Smad action have been proposed; however, these models are basically qualitative.
M, Funaba, L S, Mathews
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Expression and Purification of Thioredoxin Fusion Proteins
Current Protocols in Protein Science, 1994AbstractThis unit describes a gene fusion expression system that uses thioredoxin, the product of the Escherichia coli trxA gene, as the fusion partner. The inherent thermal stability of thioredoxin and its susceptibility to quantitative release from the E.
J, McCoy, E, La Ville
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Heterologous expression, purification, and characterization of proteins in the lanthanome
2021Recent work has revealed that certain lanthanides-in particular, the more earth-abundant, lighter lanthanides-play essential roles in pyrroloquinoline quinone (PQQ) dependent alcohol dehydrogenases from methylotrophic and non-methylotrophic bacteria.
Emily R, Featherston +3 more
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